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Biomedical subjects

P C Scriba

Publications and source records attributed to P C Scriba.

At least 127 records · Page 7Linked to original sources
PubMed1977

Prolactin: a diabetogenic hormone.

During an oral glucose tolerance test (OGTT) glucose and insulin levels were measured in 26 patients with prolactin-producing pituitary tumours without growth hormone excess. Basal glucose and insulin levels did not differ from the values of an age-matched control group. After glucose load the hyperprolactinaemic patients showed a decrease in glucose tolerance and a hyperinsulinaemia. Bromocriptine (CB 154), which suppressed PRL, improved glucose tolerance and decreased insulin towards normal in second OGTT. Human PRL or CB 154 had no significant influence on insulin release due to glucose in the perfused rat pancreas. These findings suggest a diabetogenic effect of PRL. CB 154 might be a useful drug in improving glucose utilization in hormone-active pituitary tumours.

Adenoma↗
PubMed1976

[Successful treatment of thyroid storm by continuous plasmapheresis with a blood-cell separator (author's transl)].

Continuous plasmapheresis with the IBM blood cell separator showed to be a successful therapy in a 26 year old patient with thyroid storm. The patient regained consciousness already during plasmapheresis whereby 5 liters of plasma were exchanged within 3.5 h. Although 633 mug thyroxine (T4) and 13.6 mug triiodothyronine (T3) were removed with the 51 plasma, no fall occurred in total serum T4 and T3 levels, probably due to the rapid reflux of thyroid hormones from the tissue into the intravascular space. Though total T4 and T3 did not change, there was a significant fall in free thyroid hormone levels documented by reduction of T3-uptake test and particularily of urine T4 and T3 excretion. These findings show that the elevation of thyroid hormone binding sites with donor plasma is even more important than the removal of thyroid hormones.

Adult↗
PubMed1976

Recurrent goiter, hyperthyroidism, galactorrhea and amenorrhea due to a thyrotropin and prolactin-producing pituitary tumor.

A 22-year-old woman with recurrent goiter, hyperthyroidism, galactorrhea, and amenorrhea due to a pituitary tumor is described. She had been treated surgically twice for recurrent goiter with tracheal compression. Despite clinical signs of hyperthyroidism and slightly elevated plasma thyroid hormone levels (T4: 11 mug/dl; T3: 189 ng/dl), without thyroid hormone replacement therapy the basal TSH level was elevated up to 23 muU/ml and could not be suppressed by exogenous thyroid hormones: even when the serum thyroid hormone levels were raised into the thyrotoxic range (T4: 16.2 mug/dl T3: 392 ng/dl), the basal TSH fluctuated between 12 and 29 muU/ml. The basal PRL level was elevated up to 6000 muU/ml. The administration of TRH (200 mug iv) led only to small increments of TSH and PRL levels. Bromocriptin (5 mg p.o.) or l-dopa (0.5 g p.o.) suppressed TSH and PRL values significantly. After transsphenoidal hypophysectomy, TSH and PRL were below normal and the patient development panhypopituitarism. The adenoma showed two cell types which could be identified as lactotrophs and thyrotrophs by electronmicroscopy and immunofluorescence. From these data we conclude that the patient had a pituitary tumor with an overproduction of thyrotropin and prolactin.

Adenoma↗
PubMed1976

[Simultaneous radioimmunassay for urinary thyroxine (T4) and triioldothyronine (T3) (AUTHOR'S TRANSL)].

A radioimmunoassay for the determination of thyroxine (T4) and triiodothyronine (T3) in urine was developed. The extraction of a sample, the incubations with T3-und subsequently T4-antibody and the elutions of the respective bound fractions are performed all on the same Sephadex column. This principle can be applied to as many as 120 simultaneous determinations. The interassay coefficients of variation were 20.1% for T3 and 10.6% for T4, respectively. The recovery of standards added to urine was 107 +/- 8% (mean +/- SD) for T3 and 102 +/- 8% for T4. The 24 h collections of urine from healthy controls contained 1.70 +/- 0.40 mug T3 and 1.44 +/- 0.51 mug T4 (mean +/- SD, N=20). Three hourly fractions of the urinary excretion collected from 6 a.m. through 9 p.m. showed a significant (p less than 0.0005) elevation of the T4 excretion as compared to the night fraction, whereas an increased T3 excretion was only observed from 6-9 P.M. The T4 excretion was reduced in primary hypothyroidism (0.48 +/- 0.47 mug per 24 h, p less than 0.0005), whereas the T3 excretion was not that markedly reduced (1.30 +/-0.80 mug/d), pointing to the known residual T3 secretion. A reduced T4 excretion (0.85 +/- 0.34 mug/d, p less than 0.00005) contrasted with the normal T3 excretion in patients with endemic goiter, indicating the known preferential T3 secretion in iodine deficiency states. T3 thyrotoxicosis was accompanied by an elevated T3 excretion with normal urinary T4. Normal excretion of T4 and T3 were observed in patients with persistantly suppressed serum TSH levels after successful treatment for hyperthyroidism.

Circadian Rhythm↗
PubMed1976

[Cooperative survey for the determinations of L-triiodothyronine (T3) and L-thyroxine (T4): critical evaluation of methods employed (author's transl)].

A cooperative survey of the radioimmunoassays for triiodothyronine was performed among 30 participating laboratories from the Thyroid Association of the German Society for Endocrinology. A total of 16 serum samples were sent frozen to each laboratory for the determination of both triiodothyronine (T3) and thyroxine (T4). It was possible to calculate the recovery rates of the participants from the values determined for an internal standard curve, which was hidden within the 16 samples and was made up by addition of known amounts of T3 and T4, respectively, to an untreated serum low in T3 and T4 (recovery curve). In general, most participants overestimated T3 levels, whereas T4 was determined falsely low. The intraassay coefficient of variation (CV) was determined from triplicate readings of three identical sera included within tor T3, and 4.5 and 30% for T4, respectively. There was no significant cross reaction for T4 in the T3 assays. However, most T3 assays were significantly influenced by variations of the thyroxine binding globulin (TBG) levels between different samples, that were tentatively included. It is suggested, that an improvement of the accuracy of the T3 radioimmunoassays necessitates either prior extraction of T3 or antibodies of higher avidity.

Cross Reactions↗