[Histochemical study of chicken intestinal mucosa carried out using wheat germ agglutinin (WGA)].
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Animals↗
Biomedical subjects
P Bufo
Publications and source records attributed to P Bufo.
[Gastric dysplasia and carcinoma: analytical morphometry of the nuclear form].
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Adenocarcinoma↗
[Evaluation of the inclination value in histological sections of formations that can be restructured into cylinders].
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Histological Techniques↗
[Effects of tubulo-deferens stenosis secondary to intraserosal proximal hemideferentectomy and end-to-end tubulo-vasostomy on the testicle of the rat. Histopathological aspects].
The histopathological aspects of the testis in rat after intra-serous proximal hemideferentectomy and end-to-end tubulo-vasostomy are evaluated. The histomorphometric variations of the convoluted seminiferous tubules and the quantitative modifications of the stages of spermatogenesis are correlated with the obstruction's degree of the tubulo-deferential anastomosis.
Animals↗
Morphometric analytical evaluators of lymphoid populations in nonneoplastic lymph nodes.
A morphometric analysis using an Apple II microcomputer and shape analytical morphometry (SAM) software was performed of the nuclear shape in the lymphoid population of normal (nonneoplastic) lymph nodes to provide baseline parameters useful for distinguishing between different subtypes of lymphomas. Fourier harmonic analysis and two indices, a contour asymmetric evaluator (CAE) and a shape asymmetric evaluator (SAE), are proposed as non-dimensional shape parameters. Results obtained with the CAE and SAE indices indicate that they represent sensitive shape descriptors able to reveal even subtle irregularities of nuclear outline. With these parameters, centrocyte nuclei were easily differentiated from those of all other lymphoid cells while an interesting close relationship was found between lymphocytes and centroblasts. Lymphocyte nuclear shape was shown to be less regular than generally reported, suggesting the existence of subtle differences between stimulated and unstimulated nuclei. Immunoblast nuclei were shown to have the most regular shapes.
Cell Nucleus↗
[Changes in the transparency of dentin: computerized densitometric analysis].
Computerized densitometry was utilized to carry out a quantitative and distributive study of dentin transparency in dental roots from different age individuals. By means of original software S.A.M. (Shape Analytical Morphometry) a densitometric reading of transilluminated whole dental root was performed and so obtained densitometric matrixes were worked for grey level classification and correspondent areas evaluation, false colour restitution, pseudotridimensional and row/column barr graphs, isodensity outlines and density inclination maps. Results are discussed for both age evaluation in forensic application and for altered dentin recognition in pathological conditions.
Adult↗
[Definition of the analytical relation between the surface and internal perimeter of seminiferous tubules from subjects with normal spermatogenesis].
Luminal tubular measurements are executed in testis with normal spermatogenesis to individuate, by computerized morphometric method, the ratio between these two values. Statistical evaluations and calculation of the regression curves demonstrate that this ratio is well described by means of an exponential equation which coefficients are determined. This parameter results very useful in the diagnosis of Sertoli cell only syndrome and maturation arrest both characterized by tubular lumen modification.
Biometry↗
[Description of the shape of carcinomatous nodules of the breast by means of high degree polynomial equations and Fourier harmonic analysis].
Breast cancer nodule shape has been investigated by two analytical procedures: upper degree polynomials and harmonic Fourier analysis. An automized method (TV camera interfaced with a computer) has been employed to find the coefficients of kth order equation giving the best fit. Then the difference between real node contour and function curve has been calculated and submitted to harmonic analysis. This gives the sine/cosine coefficients of the two series and the amplitude of the harmonics. The results point out that no difference exists between metastatic and non metastatic tumors and suggest that parametrized descriptors calculated may represent a peculiar behaviour in the growth of the tumor.
Breast Neoplasms↗
[Analytical morphometry of seminiferous tubules in maturation arrest: Fourier analysis of the internal contours of tubules].
Shape of seminiferous tubuli in maturation arrest, is investigated by harmonic Fourier analysis and upper degree polynomials as attempt to find morphometric parameters useful in morphological diagnosis. Inner luminal contour is considered as a closed curve in a reference system and scattered in a series of points whose coordinates are automatically calculated. Then a software named S.A.M. (Shape Analytical Morphometry) is utilized to find the Kth-order equation and calculate the best-fit curve. Moreover an ellypse is drawn with the same barycenter and axes of original curve. These three curves (real curve, best-fit curve and ellypse) are considered as periodic functions and submitted to Fourier analysis to evaluate the coefficients of the series and the spectrum of harmonics. Among 20 contributors, the subsets of greatest amplitude are selected for comparison and classification in terms of fundamental shape and its perturbations.
Fourier Analysis↗
[Reproductive conditioning of the partridge (Perdix perdix) with GnRH and an increase in the photoperiod].
Follicular growth and egg deposition were induced in the Grey Partridge (Perdix Perdix) after stimulation with Gn-RH and artificial light. The experiment was carried out from November to December 1983, during the short day period, and during the non egg laying period for this bird-sp. Two groups consisting of ten pairs of Grey Partridge each (female and male), received 3.8 mcg of Gn-RH (pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2) every 8 hours for 10 and 15 days, respectively. With interruption of the Gn-RH treatment we observed follicular regression. For this reason the birds were stimulated with artificial light daily from the 15th day of treatment until egg deposition. Fifty-five days after the start of the Gn-RH treatment the Grey Partridge laid eggs. Forty days of this period the birds were supplemented with artificial light. Twenty pairs of Grey Partridge were used as controls. Periodically two experimental female subjects (treated and control) were sacrificed to observe the development of ovary and oviducts.
Animals↗
[Morphologico-morphometric relief of the testis in Down's syndrome].
Four cases of Down's syndrome in new-born have been thoroughly studied on the histological and morphometrical point of view. It is outlined the presence in the seminiferous tubules of abnormal spermatogonia, sometimes multinucleated and characterized by a prominent nucleolus. The Authors emphasize the conspicuous number of these hypertrophic (H) spermatogonia (usually very scanty in normal new-born) never reported in the Down's syndrome before, whose significance is not easy to explain: if they represent anomalous forms of spermatogonial cells, related to the malformative condition, or a premalignant aspect is then discussed. Morphometrical measurements of the average tubular diameter (D.T.M.) and spermatogonia content have been calculated; furthermore a microprocessor system is employed for evaluating nuclear diameter, perimeter and area not only in H spermatogonia, but also in the other germ cells observed (A and P).
Down Syndrome↗
Inflammatory fibroid polyp of the rectum.
A case of inflammatory fibroid polyp of the rectum in an 8 years old boy is reported. This lesion is not infrequently found in the gastro-intestinal tract, is very rare in childhood and has never been reported in the rectum. The pathogenetical hypothesis that fibroid represents a dynamic response to recurrent small injuries with possible different histological patterns is prospected.
Child↗
[Histologic study of 3 cases of so-called tubular adenoma of the testis].
Three cases of so called tubular adenoma in cryptorchid subjects are reported. The histological findings were tubules with a particularly small diameter containing Sertoli cells (similar to the precursor type). No elastic fibres were present in the basement membrane giving a picture similar to last term fetal and neonatal testicles. These findings are regarded as hypoplastic foci of tubules which have not developed. The relationship between Pick's adenoma and malignant Sertoli cells neoplasia is also discussed.
Adenoma↗
Cyclooxygenase-2 expression in oral squamous cell carcinoma.
Cyclooxygenase (COX), the key enzyme in prostaglandin cascade, is expressed in two isoforms: the constitutive COX-1 and the inducible COX-2. Hyper-expression of COX-2 has been implicated in the pathogenesis of colon-rectal cancer in humans but it appears to play a significant role as a tumour progression factor also in other forms of human cancer, including oral cancer. The aim of this study was to analyze the expression of COX-2, at the protein level, in 45 cases of oral squamous cell carcinoma. Standard immunohistochemical streptavidin-biotin peroxidase analysis was carried out with highly specific antibody against human COX-2 and cell specific markers, in 45 oral squamous cell carcinomas. Our study revealed a moderate to high COX-2 expression in 35 out of the 45 oral squamous cell carcinoma specimens (77.8%). COX-2 expression appeared particularly abundant in the superficial ulcerated layers of relatively well differentiated carcinomas. However, we were unable to assess any statistically significant association between COX-2 hyper-expression and tumor site, tumor grading, tumor size, presence of lymph node metastases, tumor stage and age at onset, respectively. Interestingly, COX-2 expression was detected not only in areas of epithelial dysplasia adjacent to the primary layers (86% of the cases) but also in normal-appearing epithelium at the boundaries of squamous cell carcinoma (77%), indicating a possible involvement in tumour progression by the apparently normal tissue surrounding the lesion. Moreover, intense COX-2 staining was observed in endothelial cells of intra-tumour vessels and extra-tumour vessels adjacent to the tumour nests, in a high proportion of cases (82%). COX-2 positivity was associated with CD34 and VEGF positivity, indicating that these vessels were probably neo-formed ones. From this study as well as from other works, it appears that indeed COX-2 is over-expressed in this important human malignancy. However, further studies are necessary to understand the exact magnitude of this over-expression and, mostly, the possible role of COX-2 in the pathogenesis and progression of oral cancer.
Aged↗
[Role of mast cells in the physiopathology of gastric lesions caused by Helicobacter pylori].
Routine observation of stained histological preparations for the detection of Helicobacter pylori (HP) has shown a variable presence of mast cells in the mucosa of patients suffering from gastritis. Our aim was to verify whether any correlations exist between HP positivity, type of gastritis and presence of mast cells. We examined 300 bioptic fragments of gastric mucosa obtained by diagnostic oesophagogastroduodenoscopy. The study population consisted of 88 patients with chronic superficial gastritis and 12 with chronic atrophic gastritis (59 males and 41 females). HP detection was performed on Giemsa-stained preparations. Mast cell detection was performed on Giemsa- and PAS-stained preparations. Seventy percent of chronic superficial gastritis cases were found to be HP-positive and mast cells were present in 90% of these and absent in 10%. In the chronic atrophic gastritis cases 50% were HP-positive and mast cells were present in 50% and absent in the rest. HP is regarded as the causal agent of type B gastritis. On the basis of our data a significant correlation was found (86%) between HP-positivity and presence of mast cells in the inflammatory gastric mucosa.
Adult↗
[Assessment of hepatocyte AgNORs expression in chronic hepatopathy].
The high incidence of liver chronic diseases has aroused strong interest in researching and trying to discover the biomolecular basis. In this context the study of nucleolar organizing regions could be interesting as a prognostic factor for chronic hepatitis and for liver neoplastic disease. The Authors report on the results of their study performed on 39 selected samples from 4 different inflammatory hepatic disorders.
Hepatitis, Chronic↗
[Argyrophilic nucleolar organizer regions (AgNORs) as malignancy biomarkers in colorectal neoplasms].
The high incidence of intestinal cancer has aroused strong interest in researching and trying to discover its morphologic precursors. In this contest the study of nucleolar organizing regions could be interesting as prognostic factor for bowel neoplasm and useful for differential diagnosis of intestinal diseases. The Authors report on the results of their study performed on 30 selected samples from 6 different bowel lesions.
Adenocarcinoma↗
[Expression of epithelial oncoproteins in large intestine neoplasms].
The high incidence of the colorectal cancer in the industrialized countries has drawn the interest of the scientific community to detect and investigate its morphologic precursors. Growth factors and oncoproteins could be useful to investigate the morphologic and biologic evolution of precancerous lesions of the large bowel. Therefore the Authors report the results obtained from 30 selected patients with hyperplastic and adenomatous polyps, and different histotypes of colorectal cancer, investigated using the EGF-r and p62 protein expression. The results show the potential usefulness of such markers to evaluate the biology of intestinal tumors.
ErbB Receptors↗