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P Broda

Publications and source records attributed to P Broda.

66 records · Page 4Linked to original sources
PubMed1974

Modified map positions for lac and the pro markers in Escherichia coli K-12.

Interrupted mating experiments were performed with Hfr strains H and C and three leu lac purE recipient strains derived from a common parent and carrying, respectively, the proA(-), proB(-), and proC(-) mutations. It was concluded that if leu is placed at 1.5 min and purE at 12 min from thr, the origin on the Taylor-Trotter map, lac is at about 7.5 min and the pro genes are at about 6.0, 6.6, and 8.4 min, respectively. Both conjugational and transductional data suggest that the strain carrying the proB(-) mutation also carries a second mutation close to the proA site which independently confers a Pro(-) phenotype. The times before the onset of transfer of chromosomal deoxyribonucleic acid by both Hfr strains B4 and B8 were approximately 3 min.

Chromosome Mapping↗
PubMed1974

Gross map distances and Hfr transfer times in Escherichia coli K-12.

Hfr strains B4 and B8 transfer the Escherichia coli chromosome in opposite directions, each transferring lac(+) as the last known marker. They were mated in concurrent crosses with the proA leu metE lys trp purE lac strain chi462. Analysis of the time of entry values for these markers showed that Hfr strain B8 transfers the whole chromosome more rapidly than does Hfr strain B4. In both crosses, the rate of transfer observed decelerates. If deceleration occurs as a function of the amount of chromosome transferred, the data are consistent with the markers examined being very accurately placed on the Taylor-Trotter map of the E. coli K-12 genome.

Chromosome Mapping↗
PubMed1968

Ribonucleic acid synthesis and glutamate excretion in Escherichia coli.

Cultures of Escherichia coli excreted glutamate into the medium when protein synthesis was blocked in RC(rel) strains or when it was blocked with chloramphenicol in either RC(str) or RC(rel) strains. Both of these conditions resulted in continued ribonucleic acid (RNA) synthesis in the absence of protein synthesis. Glutamate was also excreted by both RC(str) and RC(rel) strains when RNA synthesis was inhibited by uracil starvation or by treatment with actinomycin D. It is proposed that, in each of these cases, glutamate excretion resulted from an increase in the permeability of the cell membrane.

Alcohols↗