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Biomedical subjects

P Bottermann

Publications and source records attributed to P Bottermann.

At least 37 records · Page 2Linked to original sources
PubMed1981

[Routine determination of HB A1 by micro-column technique (author's transl)].

For routine determination of glycosilated hemoglobin (HB A1) it is necessary to have a quick and easy assay which allows analysis of a greater number of samples. Taking into consideration known methods for hemoglobin chromatography a micro-column technique has been developed, which separates the glycosilated hemoglobins AIa+b+c from the main hemoglobin fraction HB AII. Micro-columns (7.0 x 1.3 cm) are filled up to a bed-height of 3 cm with Bio-Rex 70. Hemoglobin is given to these in hemolysate from (1,8 mg/200 microliter) and separated with two buffers of different ionic-strength and different pH-values into two fractions HB A1 and HB AII. With exact standardisation of the elution volumes (18 ml), the elution temperature (21.5 degrees C) and pH-value (6.74) of the first elution buffer a high reproducibility of the results is possible (Intraassay-VK: 2.38%; Interassay-VK: 3.68%). The optical density of the hemoglobin fractions is read for cyanhemoglobin at 413 nm or 415 nm or for cyanmethemoglobin at 419 nm. The HB A1-concentration is given as a percentage of the total hemoglobin. This method enables the determination of HB A1-value of 40 blood samples in triplicate within four hours. The normal value derived from 25 healthy and normal weight volunteers is 6.88 +/- 0.41% (mean +/- S.D.), from which an upper normal range of 7.7% has been calculated. In 103 patients with unimpaired glucose tolerance values were observed in similar range: 6.45 +/- 0.61% (mean +/- S.D.). In 121 diabetic patients with varying metabolic control HB A1-values up to 20.58% were noted.

Adolescent↗
PubMed1981

[Pharmacokinetics of insulin administered intraperitoneal in a bolus form (author's transl)].

Although carbohydrate-intake and subcutaneous insulin injection in the insulin dependent diabetics are brought to match with one another, there is often an incongruity between the momentary insulin need and the actual insulin supply, because insulin is resorbed relatively slow from the subcutaneous injection site. While the plasma insulin concentration in healthy persons after carbohydrate-intake reaches its maximum after ca. 45 min, the maximum insulin concentration after subcutaneous application of regular insulin is observed only after ca. 2 h. For this reason, we studied whether a faster rise in the insulin concentration can be obtained by intraperitoneal bolus application of insulin. 5 metabolic healthy volunteers received 20 UI regular insulin diluted in isotonic saline solution injected intraperitoneal. The insulin concentration in the peripherovenous system was examined. In order to prevent severe hypoglycaemia the test persons were connected to a Biostator (so-called artificial Beta-cell), which delivered glucose automatically when the blood glucose concentration fell below the 60 mg/dl - level, and thus avoided a drastic fall of the blood glucose. A rapid increase in the plasma insulin concentration was observed within a few minutes after the intraperitoneal bolus injection of insulin. After ca. 20 min the maximal insulin concentration was reached. Already after ca. 2 h the plasma insulin levels fell off and approached the initial values. Consequently, the changes of insulin concentration after intraperitoneal bolus application of insulin correspond widely to the insulin curve characteristic of metabolic healthy persons after carbohydrate-intake. Thus, the intraperitoneal bolus injection of insulin presents a mode of application, which must be pursued further in the treatment of insulin dependent diabetics.

Adult↗
PubMed1980

Effects of low-dose gestagen (lynestrenol 0.5 mg) and combination-type oral contraception (lynestrenol 1 mg and ethinyloestradiol 0.05 mg) on blood glucose and serum insulin levels after a glucose load.

Forty 3-h oral glucose tolerance tests (OGTTs) were performed in 10 apparently healthy female volunteers aged 21--34 years, each serving four times as her own control. Each subject was taking either a low-dose gestagen contraceptive (lynestrenol 0.5 mg) or a combination-type pill (lynestrenol 1 mg + ethinyloestradiol 0.05 mg) alternatingly in four consecutive treatment cycles. Blood glucose and serum insulin did not differ significantly with either contraceptive (paired t-test).

Adult↗
PubMed1980

The influence of high doses of oral medroxyprogesterone acetate on glucose tolerance, serum insulin levels and adrenal response to ACTH. A study of 17 patients under treatment for endometrial cancer.

Seventeen women aged 55 to 76 years who had been treated for endometrial cancer by surgery or radiotherapy or a combination of both were given 300 mg of medroxyprogesterone acetate (MPA) daily by mouth. Before treatment and again during the 3rd week of treatment an oral glucose tolerance test (with measurement of serum insulin levels) and an ACTH-stimulation test were done. All blood glucose levels tended to be higher with MOA therapy and serum insulin levels were significantly increased 3 h after a glucose load. The rise of serum cortisol 30 min after ACTH-stimulation was significantly less with MPA therapy. Oral MPA thus appeared to have a glucocorticoid-like action.

Adrenal Glands↗
PubMed1980

[Temporary hepatic support by extracorporeal baboon liver perfusion in acute hepatic failure (author's transl)].

Eight patients in acute hepatic failure were treated by a total of 41 extracorporeal baboon liver perfusions. Only one has survived to the present. Parameters of good function proved to be oxygen consumption and bile production. Highly elevated aromatic amino acids decreased significantly during one single passage through an extracorporeal perfused baboon liver, as did ammonia while urea increased significantly. The prognosis of these very ill patients can be improved only when the regeneration of their damaged livers starts up again during temporary hepatic support therapy.

Adolescent↗
PubMed1979

[Intravenous and oral TRH-stimulation test: comparison of the value of both tests concerning diagnosis and therapy of thyroid diseases (author's transl)].

15 euthyroid patients, 15 patients with a so-called non toxic goiter, 7 patients with hypothyroidism and 14 patients with hyperthyroidism (Grave's disease and autonomous adenoma) were submitted to intravenous (200 micrograms) and oral (40 mg) TRH-stimulation tests. After the oral application of TRH the patients with a normal thyroid function and the patients with a goiter showed an increase of the concentration of TSH which was about 1 1/2 fold higher than after the intravenous application of TRH. The patients who suffered from hypothyroidism showed a different reaction after intravenous and oral application of TRH. The patients with a hyperthyroidism had neither after the intravenous nor after the oral application of TRH an increase of the peripheral concentration of TSH. Therefore both intravenous and oral TRH-stimulation tests seem to be apt in the same way for the diagnosis of thyroid diseases and for the control of the therapy when thyroid hormones are applied.

Administration, Oral↗
PubMed1978

[Concentration of C-peptide and insulin in serum of patients with acute virus hepatitis (author's transl)].

Intravenous glucose tolerance tests were performed in 10 patients with acute virus hepatitis. The assimilation coefficient of glucose and the level of insulin and C-peptide in serum were determined before and in the course of the glucose tolerance tests. In comparison to healthy normal weight persons C-peptide concentration in patients with acute hepatitis increased twice as high whereas the pattern of insulin secretion did not differ significantly. The higher levels of C-peptide indicate an increase of the beta-cell secretion in acute hepatitis. One could suppose an increased hepatic destruction of insulin in acute hepatitis, because there is no significant difference among the insulin levels. More likely, there is a reactive increase of secretion of the beta-cell due to a reduction of insulin sensitivity and this is indicated much better by C-peptide- than insulin levels because of the longer half live of the the C-peptide molecule.

Acute Disease↗
PubMed1978

[Radioimmuno-assay of TSH before and after TRH in 350 patients with previous resection of euthyroid goiter (author's transl)].

Radioimmuno-Assay (RIA) of Thyroid-Stimulating-Hormone (TSH) was performed before and after i.v.-injection of Thyrotropin-Releasing-Hormone (TRH) in 350 patients subsequent to previous resection of euthyroid goiter to find out the optimal treatment schedule for preventing recurrent goiter. In patients without recurrent goiter the dosis of thyroid hormone was considered to be sufficient, if the difference in TSH-levels before and after TRH (delta-TSH) was equivalent to or less than 10 microunits/ml. In patients with recurrent goiter the optimal suppressive dosis of thyroid hormone was accepted for a delta-TSH less than or equal to 2,5 microunits/ml. 126 out of 135 patients, who were set on an immediate and continuous postoperative treatment were free of goiter, 58 presented a delta-TSH less than or equal to 10 microunits/ml (average interval of treatment = 2,5 years), 68 patients had elevated delta-TSH (average interval of treatment 2,1 years) as a sign of insufficient treatment. In 9 patients recurrent goiter was detected in spite of "adequate" treatment. 41 out of 57 patients, set on delayed and partly continuous, partly discontinuous treatment had recurrent goiter (in average after 10 years). delta-TSH was not indicative. 158 patients without any treatment had borderline or slightly increased delta-TSH (in average after 10 years). 122 of these 158 patients had recurrent goiter, 36 were free of goiter. The results favor the necessity of treatment with thyroid hormones starting immediately after operation and with continuous treatment. The combined use of TSH-RIA and TRH-test reliably informs about the individual thyroid hormone dosis necessary for prevention or treatment of recurrent goiters.

Goiter↗
PubMed1978

[Effects of the beta-adrenergic blocking agent propranolol on the carbohydrate- and lipometabolism (author's transl)].

Intravenous glucose tolerance tests were performed in 17 patients suffering from hyperthyreosis before and after a week of treatment with propranolol. The blood taken until the 125. minute after the glucose load was used for the determination of serum glucose, the free fatty acids (FFA), the free glycerol and the radioimmunologically measurable insulin (IRI). The following results were received: After the treatment with propranolol the glucose tolerance decreased significantly. The insulin secretion was diminished showing a significant difference for the fifth minute after glucose injection. The concentration of the FFA remained unchanged. The levels of the free glycerol were significantly lower after the propranolol treatment than before. Though an inhibition of lipolysis was possible by the propranolol treatment the glucose tolerance did not improve due to the inhibition of insulin secretion under propranolol. Beta-adrenergic blocking agents do not lead to an essential change in the carbohydrate- and lipometobolism. Therefore, their use in hyperthyroidism is mainly justified because of the cardial symptomatology.

Carbohydrate Metabolism↗