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Biomedical subjects

P Bobbio

Publications and source records attributed to P Bobbio.

At least 73 records · Page 4Linked to original sources
PubMedDate not supplied

The effect of verapamil and diltiazem on alveolar type II cells during warm and cold metabolic ischaemia.

The alteration of cytosolic free calcium concentration is an important event during cellular ischaemia. Calcium channel blockers have been shown to be beneficial during experimental ischaemic organ protection. To investigate the mechanisms of this protection, the behaviour of type II pneumocyte cultures, subjected to warm and cold metabolic ischaemia (6 h), was studied. The cells were incubated in electrolytic solutions and treated with high doses of verapamil (10 mg/l) or diltiazem (100 mg/l). Alveolar type II epithelial cells were removed from adult rat lungs using the modified Dobbs' method. Cell viability was determined by analysis of the total protein content, and from the rate of protein synthesis as indicated by the [35S]methionine uptake assay. The results show that verapamil does not have a direct cytoprotective or cytotoxic effect on the incubated cells, but diltiazem seems to be toxic to the cells, especially during cold ischaemia when the toxicity is significant (P < 0.05). Thus, the protection from ischaemia previously attributed to calcium channel blockers is ascribed to action on the blood vessels resulting in vasodilatation, rather than to a direct influence on cytosolic free calcium homeostasis.

Animals↗
PubMedDate not supplied

Pulmonary endothelial cell modifications after storage in solid-organ preservation solutions.

During lung preservation, the vascular endothelium is probably the first site of damage and these lesions are considered the main limiting factor in solid-organ preservation. In the present study, the ultrastructural changes in the endothelial cells of human pulmonary artery hypothermically stored (at 4 degrees C) for 6 and 12 h in Euro-Collins, University of Wisconsin and Ringer-lactate solutions were compared. The arteries obtained from three patients who underwent pneumonectomy were divided into 20 segments and preserved in the three solutions mentioned. The specimens, which were fixed in osmic acid, were examined using transmission electron microscopy. Transmission electron microscopy indicated that the cells stored in the University of Wisconsin solution either for 6 or 12 h were the best preserved, while the most severely damaged cells were those stored in Euro-Collins solution, even after just 6 h. The cells stored in Ringer-lactate showed an intermediate level of damage. The data from an ultrastructural grading scale, which quantified the damage to the cytoplasm, mitochondria and nucleus, were in broad agreement with the general transmission electron microscopy observations. Analysis of variance of the grading scale data showed that there were statistically significant differences between the groups after both 6 and 12 h storage (P < 0.05).

Aged↗
PubMedDate not supplied

[Long term follow-up after radical mastectomy for breast cancer (author's transl)].

110 of 126 patients treated with Halsted mastectomy and postoperative loco-regional radiotherapy, during period 1961-1969 have been controlled. Overall 5 years survival resulted as following: Columbia Clinical Classification stage A 90,6%, stage B 66,6%, stage C 28,5%, stage D 33,3%; 10 years survival in patients operated on in the period 1961-64 was: stage A 69,2%, stage B 53,3%, stage C 40%, stage D 0%. On the basis of the satisfing results obtained the authors conclude that it appears by now unjustified to modify this method in treating stage A and B breast cancer either utilizing less extended resections, or renoncing to the postoperative irradiation.

Breast Neoplasms↗