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P Biberfeld

Publications and source records attributed to P Biberfeld.

At least 181 records · Page 10Linked to original sources
PubMed1982

Localization of T-lymphocyte areas in human lymphoid tissues by fluorochrome-labelled Helix pomatia A haemagglutinin.

Fluorochrome-labelled Helix pomatia A haemagglutinin (HP) stained T-dependent areas in cryostat tissue sections of human lymph node, tonsil, and spleen and thymocytes within thymic medulla but not within thymic cortex. Neuraminidase treatment of the tissue section was a prerequisite for the positive cell staining. The staining was cell-membrane-associated, and the histological pattern was essentially the same as that observed in parallel sections treated with monoclonal antibodies to T cells (OKT3, Leu-1). In addition, blood vessel walls and connective tissue were also stained by HP conjugates. The latter staining was independent of neuraminidase treatment, resistant to alpha-galactosidase treatment and was not blocked by pretreatment with anti-fibronectin antibody. Body types of staining reaction were specific inasmuch as unlabelled HP and the competitive sugar hapten N-acetyl-D-galactosamine blocked the reaction, Frozen, formalin-fixed tissue sections showed the same stainability and reaction pattern as unfixed sections.

Animals↗
PubMed1981

An EBV-associated, Swedish case of Burkitt-type malignant lymphoma.

Morphological, serological, DNA-homology and immunofluorescence studies of a rapidly fatal malignant lymphoma in a 25-year-old Sweden male revealed most of the characteristics of an EBV-associated Burkitt lymphoma. The various findings are discussed in relation to previously reported observations on endemic (African) and non-endemic Burkitt lymphomas.

Adult↗
PubMed1980

Technical aspects of lymphoma immunohistology.

Immunohistological techniques (i.e., based on tissue sections) for the study of human lymphoma have been developed in recent years as alternatives to immunocytochemical methods (based on cell suspensions). These techniques not only allow the important architectural features of the lymphoma sample to be preserved, but are also more convenient to use in that tissues can be processed rapidly and studied at leisure. The relative advantages and disadvantages of frozen versus fixed embedded tissue, and of immunofluorescence versus immunoenzymatic methods are reviewed, and technical aspects of proteolytic digestion (as a technique for enhancing immunohistochemical labeling) are discussed. This review concludes with a consideration of the problems encountered in the interpretation of immunohistochemical labeling for lymphoma cell immunoglobulin in paraffin sections.

Histocytochemistry↗
PubMed1980

Immunological studies on human thymus. Occurrence and distribution of immunoglobulins and immunological receptors in myasthenia gravis and control patients.

Suspended cells and tissue sections from myasthenia gravis (MG) and control thymuses were characterized for surface expression and histological distribution of immunoglobulin (Ig) and receptors for sheep erythrocytes (SE), the Fc part of IgG (Fc gamma) and of IgM (Fc mu) and complement factors C3b and C3d (CR). In sections the cortical areas from both MG and control thymuses expressed SE as well as Fc gamma receptors, whereas medullary areas only showed weak SE binding. In contrast to control patients, MG thymuses also contained Ig+ cells confined to nodular areas of the thymic medulla. These nodules stained for both IgG and IgM, and were polyclonal with respect to light chains. In several cases IgD was found as well. In addition, the nodular areas were positive for C3b and C3d receptors. In serial sections, these Ig+ CR+ nodules corresponded to morphologically characteristic lymphoid follicles. Such B cell follicles were observed in variable numbers in all MG thymuses, whether hyperplastic or with normal histology. Five thymomas gave heterogeneous results with regard to immunological markers. Comparison of methods showed that receptor and Ig indication on sections was more sensitive than corresponding tests on thymus cells in suspension.

Adult↗
PubMed1980

An immunohistological study of human lymphoma.

In this study the problems encountered in staining immunoglobulin (Ig) in sections of paraffin-embedded human lymphoma samples have been investigated. It was found that the "masking' of cytoplasmic Ig, which occurs when tissues are fixed in formol saline (the fixative employed in most previous studies), can be avoided by the use of mercury-based fixatives. When non-Hodgkin's lymphoma samples fixed in this way were studied it was found that cytoplasmic Ig labelling of both lymphoid and histiocytic cells is often attributable to non-specific uptake of serum proteins. This phenomenon probably accounts for a number of published anomalous immunoperoxidase staining results in human lymphoma (e.g. the presence of both kappa and lambda chains in the same neoplastic cell). Double immunoenzymatic labelling (using alkaline phosphatase and peroxidase) proved valuable in the elucidation of this phenomenon. When staining due to absorbed Ig was discounted it was possible to demonstrate monoclonal Ig labelling in seven out of sixteen cases of non-Hodgkin's lymphoma. In each case IgM was found in association with a single light chain type and these results were in agreement with those obtained by direct immunofluorescent labelling of cryostat sections. In a further case u chains without associated light chains were demonstrated by immunoperoxidase staining. Seven cases of Hodgkin's disease were studied by immunoenzymatic techniques. Although IgG was frequently found in Reed-Sternberg and Hodgkin's cells its presence was not attributable to non-specific uptake of serum protein since albumin was absent or only present in small amounts. These findings are in support of the macrophage origin of these cells.

Fixatives↗
PubMed1979

The basement membrane in experimentally induced atypias and carcinoma of the uterine cervix in mice. An immunofluorescence study.

Basement membrane-specific antigens of the squamous epithelium of the uterine cervix were investigated in 19 normal mice, in 7 mice with cervical atypia and in 3 mice with invasive carcinoma. Cervical atypia and carcinoma were induced by local application of benzo(a)pyrene. Basement membrane-specific antigens were demonstrated by immunofluorescence with sera from patients with bullous pemphigoid. Both normal squamous cervical epithelium and atypical cervical epithelium showed the presence of a continuous, clearly delineated basement membrane. Clusters of invasive squamous carcinoma were also surrounded by a fluorescent basement membrane which, however, appear fragmented or discontinuous. The results suggest that the ability of cervical squamous cells to secrete basement membrane antigens is not completely lost during carcinogenesis, thus substantiating our previous observations in the cervix of human subjects.

Animals↗
PubMed1979

T lymphocyte subpopulations in synovial fluid of patients with rheumatic disease.

The proportion of T lymphocytes with receptors for the Fc portion of IgG (TG cells) of IgM (TM cells) was determined in synovial fluid and blood of 16 patients with various rheumatic diseases including rheumatoid arthritis, psoriatic arthritis, and ankylosing spondylitis. The percentage of TG cells was low in all synovial fluid samples, whereas in the patients' blood the percentage was higher than or equal to the level found in the blood of healthy subjects. Eight patients also had a lower level of TM cells in synovial fluid as compared to the percentage found in the blood of healthy donors. In the patients' blood the percentage of TM cells was usually within the normal range. Thus the proportion of T cells lacking either receptor (Tnull cells) was higher in synovial fluid than in blood. This pattern of low TG cell and high Tnull cell percentages was found in the synovial fluid of patients with various rheumatic diseases and thus seems to be a general feature of chronic inflammatory joint exudates.

Arthritis, Rheumatoid↗
PubMed1979

The distribution of HLA on human lymphoid, bone marrow and peripheral blood cells.

The cellular distribution and the differential expression of HLA on cell suspensions and tissue sections has been investigated using the monoclonal antibody W6-32, which reacts with the high molecular weight chain of the major histocompatibility antigen. Lymphocytes and platelets, as assessed by autoradiographic and immunoperoxidase labeling, were the most densely labeled cells. Myeloid precursors showed more labeling than mature neutrophils. Electron microscopic immunoperoxidase labeling showed a continuous distribution of HLA antigen on lymphoid and myeloid cell membranes. Erythroid precursors (including reticulocytes), although very weakly labeled, were clearly positive, in comparison with mature erythrocytes. In the thymus, HLA-negative, thymocyte antigen-positive cells (85%) can be distinguished from HLA-positive, thymocyte antigen-negative cells (15%). By using immunofluorescence techniques on tissue sections, the former cells were shown to be cortical thymocytes and the latter medullary cells.

Antigens, Surface↗
PubMed1979

Immunocytochemical studies of human myeloma cells by light and electron microscopy.

Demonstration of atypical plasma cells in bone marrow and extramedullary sites is the conventional cytological criterion for the diagnosis of human myeloma and assessment of its development. By immunocytochemical methods, it is possible to demonstrate in the blood of myeloma patients the occurrence of mononuclear cells, which manufacture and express idiotypic determinants (Id+) of the patient's own myeloma protein. These circulating Id+ cells have a polymorphic ultrastructure ranging from that of atypical lymphocytes to lymphoblastic-lymphoplasmacytoid and plasmacytoid cells. Our observations in several patients indicate that monitoring the blood levels of Id+ and Ig+ cells is a sensitive probe for following the development of the disease.

Humans↗
PubMed1978

Characteristic surface morphology of human and murine myeloma cells: a scanning and transmission electron microscopic study.

Cells from cultured human and murine myeloma cell lines and circulating leukaemic plasma cells from four patients with generalized myeloma were studied by transmission and scanning electron microscopy and time-lapse cinematography. Both circulating and cultured cells exhibited consistent surface architectures and varying numbers of prominent blebs of different sizes were seen, in addition to microvilli. The presence of surface blebs appears to be a characteristic feature for secreting and non-secreting myeloma cells.

Animals↗