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Biomedical subjects

P Bernhardt

Publications and source records attributed to P Bernhardt.

24 records · Page 2Linked to original sources
PubMed1989

[Absorption and fluorescence spectrometry measurement of intraocular lenses].

Light absorption and fluorescence measurements can be used to detect accumulations of small molecules in intraocular lenses (IOLs). It was shown that HEMA IOLs can store and to some extent release a variety of drugs and fluorescein. PMMA and silicone IOLs, however, do not have this characteristic. UV-blocking IOLs absorb UV light completely. The absorption limit varies between 390 and 410 nm, depending on the make. Neither absorption nor fluorescence response were affected by exposure to UV light or heat.

Acebutolol↗
PubMed1989

[Natural fluorescence of nuclear cataract lenses and melanomas of the eye].

For various reasons, it is desirable to have an objective cataract classification system that does not depend on either the opinion of the physician or the patient. Since both highly molecular protein aggregates and chromophores are formed in cataractous lenses, which fluoresce under suitable stimulation, we have developed a fluorescence apparatus that illuminates the lens with five monochromatic wavelengths between 350 and 500 nm in situ. The fluorescence spectrum is recorded up to 100 ms with an optical multi-channel analyzer. This fluorescence method is non-invasive and does not harm the eye; it also uses the native fluorophore. The set of fluorescence spectra obtained by this method describes the condition of the cataractous lens both quantitatively and qualitatively. Because of the sensitivity of the method, it can also be used to assess the disease objectively in the early stages and to test and determine the effectiveness of anti-cataract drugs. Another use was discovered by using the fluorescence measurements for the diagnosis of malignant melanomas. With this equipment, melanomas in the eye and the surrounding area can be diagnosed both rapidly and precisely.

Cataract↗
PubMed1988

Calcified intralobar pulmonary sequestration.

The radiologic features of intralobar pulmonary sequestration (ILPS) have been described and include the identification of a feeding systemic artery on computerized tomography. Radiographically demonstrated calcification is rare and has been described only three times. We describe a patient with an ILPS whose radiographic findings were unusual for two reasons. First, while computed tomography (CT) demonstrated a tubular structure, it did not enhance with contrast material and thus was not considered to be a feeding vessel. Pathological examination revealed that this vessel had undergone thrombosis with subsequent organization and calcification. This finding also explains the second unusual feature, calcification, seen both on a plain chest radiograph and CT.

Aged↗
PubMed1985

Applications of fluorochromes to pollen biology. II. The DNA probes ethidium bromide and Hoechst 33258 in conjunction with the callose-specific aniline blue fluorochrome.

Techniques are described for detection of pollen grain and pollen tube nuclei using the fluorescent DNA probes ethidium bromide or Hoechst 33258, in conjunction with the aniline blue fluorochrome sirofluor, which stains the callose component of pollen tube walls and plugs. The DNA probes, which may be used either as vital stains or following fixation, permit discrimination between vegetative and generative or sperm nuclei. Double staining with sirofluor allows location of nuclei within pollen tubes grown in vitro, and when used after pollination enables the viewer to discriminate between nuclei within the pollen tube vs. nuclei of the pistil tissue.

Benzenesulfonates↗