Biomedical subjects

P BOULANGER

Publications and source records attributed to P BOULANGER.

At least 19 recordsLinked to original sources

PubMed1965

THE USE OF FLUORESCENT ANTIBODY STAINING IN THE DIAGNOSIS OF RABIES.

Brain material from 750 domestic and wild animals submitted to this laboratory for rabies diagnosis was studied by the following three methods: a) microscopic examination of Williams' stained impressions, b) mouse inoculation test, and c) microscopic examination of impressions stained with fluorescein-tagged antibodies. The purpose of this investigation was to compare the sensitivity of the fluorescent antibody technique with that of two classical methods, when applied to the routine diagnosis of rabies. From the results obtained by one or the other method of study, 175 specimens were diagnosed as positive. Of these, only 58 (33 per cent) were detected by the examination of Williams' stained impressions. On the other hand, two rabid cases were missed by the mouse inoculation test, and four by the fluorescent antibody technique. Without being completely reliable, the last two methods proved to be almost equally sensitive and much more so than the examination of Williams' stained impressions.

Animals↗

PubMed1965

HOG CHOLERA. II. RELIABILITY OF THE AGAR DOUBLE DIFFUSION PRECIPITATION TEST FOR THE DIFFERENTIATION OF H.C. VIRUS FROM OTHER INFECTIOUS AGENTS IN SWINE TISSUE.

The specificity in the agar diffusion precipitation test of the reaction between the antigen of hog cholera virus diffusing from infected tissues and its homologous antibody was verified. Alternate freezing and thawing of infected tissues was found to give optimum release of the antigen from fresh tissue frozen for 18 hours. A study of the effect of the size and age of pigs upon the diffusion of the antigen from tissues showed that tissues from pigs of less than 250 lbs. gave good results provided the tissues were from animals showing gross clinical manifestations. Specimens from infected breeding sows and dead animals usually did not give a reaction.

Agar↗

PubMed1965

STUDIES ON BLUETONGUE II. COMPLEMENT-FIXING ACTIVITY OF OVINE AND BOVINE SERA.

Sera of sheep and calves infected with the California type 10 and Cyprus type 3 viruses of bluetongue were tested by the regular and modified direct complement-fixation tests. To obtain satisfactory complement fixation it was necessary to use the latter test. Cross reactivity was found, therefore, the California type 10 antigen could be used in testing sera of animals infected with the Cyprus virus.

Animals↗

PubMed1965

HOG CHOLERA. 3. INVESTIGATION OF THE COMPLEMENT-FIXATION TEST FOR THE DETECTION OF THE VIRUS IN SWINE TISSUE.

The complement-fixation test was investigated as a means of detecting hog cholera virus in spleen from experimentally infected swine. Various methods of extracting the tissue for production of antigen are described and emphasis is placed on the necessity of using the modified direct complement-fixation test to obtain reactions. The tissue should be obtained from animals showing advanced clinical manifestations of the disease. Preferably, the tissue should be maintained frozen or at least well refrigerated. The results indicate that tissue from dead animals or from breeding sows should be avoided. The 77 per cent positive reactions obtained suggest the test could be of diagnostic value provided two or three samples are obtained from the same herd.

Animals↗

PubMed1964