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Biomedical subjects

P B Hill

Publications and source records attributed to P B Hill.

13 recordsLinked to original sources
PubMed1999

Resolution of paraneoplastic alopecia following surgical removal of a pancreatic carcinoma in a cat.

A 13-year-old female neutered domestic longhaired cat was presented with a five-month history of progressive weight loss and bilaterally symmetrical alopecia of the ventrum, limbs and perineum. The alopecic skin had a shiny appearance and hair in the non-alopecic areas was easily epilated. Fine needle aspirate cytology of a palpable cranial abdominal mass revealed it to be of epithelial or glandular origin. A pancreatic mass was excised by left pancreatectomy during exploratory laparotomy, and histopathology and skin biopsies confirmed a diagnosis of pancreatic carcinoma with concurrent paraneoplastic alopecia. No evidence of metastases was found on liver and lymph node biopsies. At re-examination 10 weeks after surgery, the hair had fully regrown. Skin signs recurred after 18 weeks and metastatic spread of the tumour was confirmed on postmortem examination. This case confirms that paraneoplastic alopecia associated with internal malignancies is a potentially reversible process if the internal neoplasm is excised.

Alopecia↗
PubMed1996

Characterization of whole-cell currents in mucosal and connective tissue rat mast cells using amphotericin-B-perforated patches and temperature control.

Rat mucosal type mast cells are thought to possess only a K+-selective inwardly rectifying (IRK) current in the resting state. We used rat-bone-marrow-derived mast cells (BMMCs) as a model of mucosal mast cells and recorded whole-cell membrane currents from cells perforated with amphotericin B. Under these conditions, both inwardly rectifying (IR) and outwardly rectifying (OR) currents were observed. The reversal potential and conductance of the IR current depended on the extracellular K+ concentration, indicating that the channel was K+ selective. The OR current was not affected by changes in extracellular K+ concentration, but lowering extracellular Cl- concentration reduced the conductance and shifted the reversal potential in a positive direction. The OR current was not affected by K+ channel blockers, but was reversibly blocked by the chloride channel blocker 4,4'-diisothiocyanato-2,2'-stilbenedisulphonate (DIDS), again indicating a Cl- conductance. The IRK current was also detected in the majority of cells using the conventional whole-cell recording configuration at room temperature. In contrast, the ORCl current was only observed in 7% of recordings made at room temperature with the conventional whole-cell voltage-clamp mode, but was detected in 66% of cells if the bath temperature was increased and the integrity of the cell's cytoplasm was preserved by using the perforated-patch technique. Under similar conditions, the ORCl current was also present in rat peritoneal mast cells, a connective tissue phenotype previously thought to have no whole-cell currents in the resting state. The role of this current and factors affecting its activation are discussed.

4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid↗
PubMed1996

Stem cell factor enhances immunoglobulin E-dependent mediator release from cultured rat bone marrow-derived mast cells: activation of previously unresponsive cells demonstrated by a novel ELISPOT assay.

Mucosal mast cells (MMC) are important effector cells in the immune response against gastrointestinal nematodes. We used cultured rat bone marrow-derived mast cells (BMMC) as an in vitro model of MMC to study the effects of the multifunctional cytokine stem cell factor (SCF) on immunoglobulin E (IgE)-dependent secretion of granule mediators. SCF (< or = 1000 ng/ml) was not a direct secretagogue for these cells, but it significantly enhanced IgE-mediated secretion of the granule constituents rat mast cell protease-II (RMCP-II) and beta-hexosaminidase from mature BMMC in a dose-dependent manner (> 10 ng/ml). Maximum up-regulation of secretion occurred after cells were pretreated with SCF (50 ng/ml) for 5 minutes before challenge with anti-IgE, but the effect then declined and was absent in cells incubated with the cytokine for 3 to 24 h. In a novel ELISPOT assay developed to identify individual BMMC secreting RMCP-II, the proportion of mature BMMC responding to anti-IgE was significantly increased by treatment with SCF. To investigate this effect further, the percentage release of RMCP-II and beta-hexosaminidase from populations of mature BMMC was directly compared to the proportion of individual cells releasing RMCP-II as detected by ELISPOT. The release of both mediators was enhanced by SCF, and the increased percentage release reflected both an increased proportion of secreting cells, and enhanced mediator release from individual cells. These results suggest that SCF can enhance IgE-dependent mediator release from BMMC not only by augmenting the secretory response from individual cells, but also by activating previously unresponsive cells.

Animals↗
PubMed1995

Concentrations of total serum IgE, IgA, and IgG in atopic and parasitized dogs.

Concentrations of total serum IgE, IgA, and IgG were measured in 36 atopic and 16 parasitized dogs, and compared them with 30 healthy control dogs. IgE was measured using enzyme-linked immunosorbent assay. IgA and IgG were measured using radial immunodiffusion assays. Mean total serum immunoglobulin (Ig) E concentrations in healthy, atopic and parasitized dogs were 7.1 units (U) ml-1, 5.8 U ml-1 and 14.3 U ml-1, respectively. Mean total serum IgA concentrations in the same groups were 103.3 mg dl-1, 63.2 mg dl-1 and 67.3 mg dl-1, respectively. Mean total serum IgG concentrations were 1066 mg dl-1, 1621 mg dl-1 and 1480 mg dl-1 in the three groups. There was no significant difference in IgE concentrations between these groups of dogs. IgA levels were significantly lower in atopic and parasitized dogs compared with healthy dogs (P < or = 0.05), whereas IgG levels were significantly higher in the atopic and parasitized dogs (P < or = 0.005). These results suggest that measurement of total serum IgE would be of no benefit in the preliminary clinical investigation of a suspected atopic dog. The lower IgA and higher IgG concentrations in both atopic and parasitized dogs suggest that similar regulatory mechanisms governing immunoglobulin synthesis occur in canine allergic and parasitic disease, promoting IgG synthesis but down-regulating IgA production.

Animals↗
PubMed1994

Canine pyoderma.

Explore the source record for details and available documents.

Animals↗
PubMed1994

Quantification of serum total IgE concentration in dogs by use of an enzyme-linked immunosorbent assay containing monoclonal murine anti-canine IgE.

A method for quantification of serum total IgE concentration in dogs by use of an ELISA containing monoclonal mouse anti-canine IgE was developed. Microtitration plates were coated with monoclonal mouse anti-canine IgE. Test sera and reference serum dilutions were added, followed by biotinylated monoclonal mouse anti-canine IgE. Avidin-alkaline phosphatase conjugate was added, and color development was measured spectrophotometrically, using a microtitration plate reader. Quantitative results were obtained by assigning to a reference serum a value of 100 IgE units/ml. Absorbance values of unknown samples were converted into IgE units by comparison with a standard curve generated by measurement of reference serum dilutions. Intra- and interassay coefficients of variation were 5 and 7%, respectively, and assay sensitivity was 1 U/ml. The assay was used to establish a normal range for total IgE concentrations in 30 healthy dogs. Total IgE concentration in healthy dogs followed a skewed distribution and ranged from < 1 to 91.2 U/ml, with a geometric mean value of 7.1 U/ml. The IgE concentration was remarkably stable in serum samples subjected to 25 freeze/thaw cycles or incubation at approximately 25 C (room temperature) for up to 10 days. Comparison of total IgE concentrations in 23 serum samples assayed by use of double-overlay radial immunodiffusion and ELISA yielded correlation coefficient of 0.94. Comparison of the reference serum standard curve with serial dilutions of a purified IgE solution of known concentration yielded a range of values for the IgE unit of 0.7 to 2.0 micrograms.

Animals↗
PubMed1986

Gonadotrophin release and meat consumption in vegetarian women.

Many factors including diet modify the hypothalamic-pituitary axis and menstrual periodicity. We have determined the effect of a daily meat or a soybean supplement in rural vegetarian Black women on the length of the menstrual cycle and the episodic and luteinizing releasing hormone stimulated release of luteinizing hormone. The daily meat but not soybean supplement increased the length of the menstrual cycle (p less than or equal to 0.01), increased the release of LH (p less than or equal to 0.01), and decreased the stimulated release of LH in the luteal phase (p less than or equal to 0.01). These changes are opposite to those reported previously in the Caucasian women fed a meatless diet. Thus addition of meat in the diet modifies the episodic release of gonadotrophins and follicular maturation. The importance of a carbohydrate diet preferentially maintaining CNS-rhythmicity is suggested.

Adult↗
PubMed1979

Effect of a vegetarian diet and dexamethasone on plasma prolactin, testosterone and dehydroepiandrosterone in men and women.

This study reports the effect of a vegetarian diet and dexamethasone administration on the hormone status of healthy Caucasian men and premenopausal women. A lower nocturnal release of prolactin and testosterone occurred in men fed a vegetarian diet, while in women, dexamethasone administration decreased the nocturnal release of prolactin and caused a greater decrease of plasma dehydroepiandrosterone (DHEA). These results show that diet modification can induce hormonal changes, If similar changes occur in patients with breast and/or prostatic cancer, diet modification may be of benefit in these patients with tumors known to be hormonally dependent.

Adult↗