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Biomedical subjects

P Avner

Publications and source records attributed to P Avner.

97 records · Page 6Linked to original sources
PubMed1979

Distinctive properties of fucosyl glycopeptides on human teratoma cells.

Fucose-labeled glycopeptides from four human teratoma cell lines of independent origin show similar elution profiles on Sephadex G-50 column chromatography. The fucosyl glycopeptides elute in two major regions: one near the void volume, the other in fractions corresponding to a molecular weight of 2500-3000. These elution profiles are very different from those obtained with the other human cell lines examined which included 3 lymphomas, 2 colon carcinomas, and HeLa. The elution profiles of the human teratomas, however, show remarkable similarities to those obtained with murine embryonal carcinoma cell culture and early mouse embryos. These results suggest that the excluded G-50 fraction may well contain glycopeptides playing a role in mammalian embryogenesis.

Carcinoma↗
PubMed1976

Cell lines derived from teratocarcinomas.

A variety of cell lines have been isolated in vitro from transplantable teratocarcinomas. Some of them correspond to embryonal carcinoma (EC) cells. They are malignant and represent the stem cells from which all differentiated tissues derive in the tumor or in vitro. EC cells share some biochemical and antigenic properties with multipotential embryonic cells. From one of these EC cell lines, variants have been isolated in vivo and in vitro. Some are of EC type but restricted in their pattern of differentiation; others are altered in their tumorigenicity or in their antigenic characteristics. Another class of such variants corresponds to non-EC types. The most interesting ones correspond to tumoral lines of extra-embryonic tissues. All these cell lines constitute a valuable material for the study of mouse development and differentiation.

Acid Phosphatase↗
PubMedDate not supplied

Localization of the region homologous to the Duchenne muscular dystrophy locus on the mouse X chromosome.

Recent progress has resulted in part of the gene mutated in Duchenne and the milder Becker muscular dystrophies being cloned and has suggested that the gene itself extends over 1,000 to 2,000 kilobases (kb). To study how mutations in this gene affect muscle development and integrity, it would be of interest to have available a mouse model of the human disease. The mouse mdx mutation affects muscle and confers a mild dystrophic syndrome, but it is not clear whether this mutation is equivalent to Duchenne/Becker muscular dystrophy in man. Here we describe the use of two sequences from the human Duchenne muscular dystrophy (DMD) gene that cross-hybridize to mouse X-linked sequences to localize the gene homologous to DMD in the mouse. Both sequences map to the region of 10 centimorgan lying between the Tabby (Ta) and St14-1 (DxPas8) loci, close to the phosphorylase b kinase locus (Phk). By analogy with the human X-chromosome, we conclude that the region in the mouse around the G6pd and St14-1 loci may contain two genes corresponding to distinct human myopathies: Emery Dreifuss muscular dystrophy which is known to be closely linked to St14-1 in man and the DMD homologue described here.

Animals↗
PubMedDate not supplied

Developmentally controlled transcription of HLA and beta 2-microglobulin genes in human teratocarcinoma lines.

Poly(A+) RNA have been extracted from the human adult cell lines Raji and D98 (HeLa) and from the teratocarcinoma-derived cell lines TeraI and PA1. The TeraI cell line expresses many markers in common with undifferentiated mouse embryonal carcinoma cells and has been previously shown to be less differentiated than PA1. Only small amounts of poly(A+) RNA extracted from TeraI were found to hybridize with human HLA and beta 2-microglobulin (beta 2m) cDNA probes, while poly(A+) RNA from PA1 and the two other adult cell lines exhibited intense hybridization to these two probes. Our results correlate with the differentiation status of the various cell lines as defined by serological and biochemical analysis and suggest that HLA and beta 2m gene expression is mainly controlled at the transcriptional level.

Animals↗