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P Argibay

Publications and source records attributed to P Argibay.

21 records · Page 2Linked to original sources
PubMedDate not supplied

Inhibition of allospecific response in pancreatic islet transplantation: the glycan approach.

UNLABELLED: Host resistance has precluded clinical islet transplantation from becoming a consistent therapy for type I diabetic patients, mainly due to both specific and nonspecific processes. O-glycosylated proteins have a primary role in immunologic synapses. Therefore, we investigated the effects of a putative immunomodulatory effect of the cleavage of these molecules on islet allotransplantation. METHODS: Murine islets were treated with O-sialoglycoprotein endopeptidase. Three endpoints were studied: (1) proliferation in allogeneic mixed islet mononuclear cell reactions using treated and control irradiated islets as stimulator cells of mononuclear cells; (2) expression of IA-d on monocytes using 48-hour transplants of treated versus control mouse islets into subcutaneous capsules; (3) posttransplant graft function in an in vivo model of islet allotransplantation. Treated and control islets were transplanted in diabetic mice treated daily with cyclosporine. Glycemia was monitored to determine diabetes reversion. RESULTS: The allogeneic proliferative response was maximal when allogeneic mononuclear cells were mixed with control islets; it was significantly decreased with treated islets. Mean proliferative inhibition rate of treated vs. control was 62%. IA-d expression on monocytes was maximal in control islets. Reversion was significantly different for treated versus control islets with its duration varied from 3 to 7 days. CONCLUSION: These results suggest that treatment of islets with O-sialoglycoprotein endopeptidase may modulate allogeneic immunologic reactions.

Animals↗
PubMedDate not supplied

[Contact cholangiography in the pathology of the terminal choledochus].

We performed 25 operative contact cholangiographies in patients operated upon for biliary and/or pancreatic diseases. With this technic we obtained better operative radiologic studies of the distal portion of the common bile duct and of the proximal segment of duct of Wirsung; these images were sharper than that of the conventional operative cholangiography, giving a better idea of the real size of both biliary and pancreatic ductal size. The technic is performed placing a small dental occlusal X-ray plates that measures 5 x 7 cm behind the mobilized second portion of the duodenum and head of the pancreas by a Kocher maneuver and iodized contrast medium is injected through the cystic duct; we performed first a conventional operative cholangiography followed by contact cholangiography. In this series of patients we compared the results obtained with both types of cholangiographies, concluding that when there are doubts in the interpretation or in the diagnosis of the biliary condition at the distal portion of the common bile duct or at the ampullary region in a conventional operative cholangiography, it should be performed a contact cholangiography since this will yield better and sharper images.

Cholangiography↗