Biomedical subjects

P Alm

Publications and source records attributed to P Alm.

225 records · Page 13Linked to original sources

PubMedDate not supplied

Increased plasma prolactin levels in a group of men with breast cancer--a preliminary study.

Gonadal and hypophyseal hormones were investigated in 15 males with breast cancer and 15 tumour referents, on average 1 month postoperatively. Plasma prolactin was found to be significantly more often elevated in men with breast cancer compared with referents (p less than 0.005). Another group of men with breast cancer disclosed a tendency for lower S-FSH levels compared with the referents (p less than 0.01). No significant difference was seen between cases and referents regarding S-LH, p-estradiol or p-testosterone. The size of the primary breast tumour was correlated with a higher prolactin level. The findings lend support to a theory implicating prolactin and possibly prolactinomas as a risk factor for the disease in males.

Aged↗

PubMedDate not supplied

Endometrial hyperplasia: a prospective randomized study of histopathology, tissue steroid receptors and plasma steroids after abrasio, with or without high dose gestagen treatment.

274 patients with spontaneous endometrial hyperplasias were randomized into two groups: one treated with only abrasio, and another with additional 500 mg medroxyprogesterone acetate (MPA) twice weekly for 3 months. Total disappearance of all types of hyperplasias was seen following MPA treatment, whereas with only abrasio about 60% were removed suggesting that MPA does not have to be generally given to eliminate endometrial hyperplasias. Quantitative determinations of tissue steroid receptors and plasma steroids do not seem to be useful for the selection of patients in need of gestagen treatment.

Androstenedione↗

PubMedDate not supplied

The effect of continuous bleomycin infusion on the growth and cell kinetics of heterotransplanted squamous cell carcinoma of the head and neck.

The schedule-dependent effect of bleomycin (BLM) on human squamous cell carcinoma (SCC) was investigated using a xenografted SCC line originating from the head and neck region. A total dose of 200 mg BLM/kg was administered according to one of three different schedules: one single i.p. injection; i.p. injection every four hours for seven days; and continuous s.c. administration for seven days via osmotic mini-pump. Both single dose treatment and iterated injections caused significant retardation of tumour growth, and continuous infusion had a more pronounced effect, almost completely retarding increase in tumour volume; these differences in effect in between the treatment schedules were not, however, statistically significant. The effect of continuous BLM infusion on cell cycle phase distribution and histopathological picture was also recorded. During the first three days of infusion, there was an accumulation of cells in Go/G1-phase paralleled by a depletion of cells in S-phase. This was followed by a normalisation during the rest of the infusion period, concomitant with a transient increase of the cells in G2-phase. Histopathologically, there were no changes during the first four days. A swelling of the cytoplasm cold then be seen; and after seven days, scattered groups of necrotic cells were observed which later formed necrotic foci. It is noteworthy that the major perturbances in cell cycle phase distribution preceded both retardation of tumour volume growth and histopathological changes.

Animals↗

PubMedDate not supplied

Changes in tumor morphology in one human endometrial carcinosarcoma heterotransplanted into nude mice.

Tissue material from one human endometrial carcinosarcoma was transplanted into nude mice. In the third passage two independent tumor lines developed, which continued to grow independently. After the fifth passage one line could be diagnosed histopathologically as a poorly differentiated adenocarcinoma without sarcoma components, while the other line was still a carcinosarcoma. It was assumed that the development of two histologically different tumor lines could be due to clonal selection induced by the new host.

Adenocarcinoma↗

PubMedDate not supplied

Heterotransplantation of human malignant endometrial tumors into nude mice.

Seven of 13 human malignant endometrial tumors heterotransplanted into nude mice were accepted. The graft acceptance was significantly higher in papillary than in endometroid adenocarcinomas (p less than 0.01), whereas there was no observed relation between transplantability rate and other clinical prognostic factors. Of the carcinosarcomas and endometroid adenocarcinomas only PgR negative tumors were accepted.

Animals↗

PubMedDate not supplied

Flow cytometric DNA analysis of normal and cancerous human endometrium and cytological-histopathological correlations.

The DNA content in individual cells from 112 histopathologically normal endometria and from 222 malignant endometrial tumors was measured using flow cytometry. In the normal endometrial cases only single DNA peaks were found, all in the diploid region, and the range of DNA index values was used for defining the limits for diploid tumor cases. In most cases with aneuploid cell populations, an additional peak was found in the normal diploid region. However, combined cytological and histopathological analysis showed that a majority of these diploid cells were to be considered as tumor cells. Aneuploid cell populations were found in 43% of malignant endometria; in the remaining endometrial carcinomas, the flow cytometrical findings showed no differences compared to those of benign tissue. Flow cytometry in this respect did not prove useful as a diagnostic screening method. Ploidy aberrations were correlated to histopathology. Aneuploidy was more common (62%) in poorly differentiated tumors than in highly/moderately differentiated tumors (29%). Two or more aneuploid cell populations were found in 6% of the cases. No difference in aneuploidy was found between FIGO stage I and II (36% and 34%), but aneuploidy was more frequent in stage IV (86%). In normal endometria the fraction of cells with DNA content corresponding to S-phase (S-fraction) was 9.7% on average in the proliferative phase and 6.2% in the secretory phase. In well and moderately differentiated diploid tumors the S-fraction was about the same (8.8% and 9.2%), but in poorly differentiated tumors it was significantly higher (12-16.5%).

Adenocarcinoma↗

PubMedDate not supplied

Prognostic value of flow cytometrical DNA measurements in stage I-II endometrial carcinoma: correlations with steroid receptor concentration, tumor myometrial invasion, and degree of differentiation.

The prognostic value of flow cytometric DNA measurements of individual tumor cells was studied in 166 patients with endometrial carcinoma stage I-II. Follow-up time was 1-46 months and 110 patients were followed for more than 2 years. Three different ways of estimating DNA ploidy were evaluated, and the use of normal endometria in defining diploidy was considered the best. DNA-ploidy had a much more marked prognostic value in terms of 2-year recurrence-free survival than other known prognostic parameters, such as degree of differentiation, range of myometrial invasion and estradiol and progesterone receptor concentrations. The proliferative activity as determined by DNA flow cytometrical analysis was not correlated with prognosis.

Aneuploidy↗

PubMedDate not supplied

Relapse of endometrial carcinoma related to steroid receptor concentration, staging, histologic grading and myometrial invasion.

Estradiol and progesterone receptor concentrations were positively correlated to the stage of the disease and to the grade of the tumors, but not to myometrial invasion. Myometrial invasion of more than the inner third and degree of differentiation are both important prognostic factors. By combining low estradiol receptor concentration and the degree of myometrial invasion in poorly differentiated adenocarcinomas, stage I-II, it was possible to identify a group of patients containing a high percentage of relapses. This group consisted of 60% of all patients with poorly differentiated carcinoma stage I-II, who had a relapse rate of almost 50%, and were thus suitable for aggressive initial treatment.

Adult↗

PubMedDate not supplied

Correlation between estradiol-17 beta and progesterone cytosol receptor concentration, histologic differentiation and 3H-thymidine incorporation in endometrial carcinoma.

Women treated with estrogen exhibit a higher risk of developing endometrial cancer. steroid hormones exert their effects on target tissue through specific cytosol receptor protein. Knowledge of this steroid receptor concentration in endometrial carcinoma might facilitate the treatment of recurrences. We have compared the concentrations of endometrial estrogen and progesterone cytosol receptors with the histologic grade of endometrial carcinoma as well as the rate of 3H-thymidine incorporation. We found a significant difference in receptor concentration between well-moderately differentiated tumours and poorly differentiated ones. No correlation was found between 3H-thymidine incorporation rate and differentiation. A positive correlation between thymidine incorporation rate and progesterone receptor concentration was noticed. The concentration of receptors varies within a wide range of each group of differentiation; thus 23% and 4% of the poorly differentiated tumours had higher concentration of estradiol and progesterone receptors respectively than the median values for well differentiated tumours.

Cytosol↗