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Biomedical subjects

P Albrecht

Publications and source records attributed to P Albrecht.

At least 145 records · Page 8Linked to original sources
PubMed1971

Intracerebral inoculation of rhesus monkeys with a strain of measles virus isolated from a case of subacute sclerosing panencephalitis.

Measles virus isolated from the brain of a patient with subacute sclerosing panencephalitis was injected intracerebrally (ic) into 34 rhesus monkeys. Groups of these animals were injected with measles antigen in Freund's complete adjuvant or treated by schedules used for suppression of the general or cell-mediated immune responsiveness. In another group of animals, experimental allergic encephalitis was induced parallel with measles infection. Measles virus was isolated from the brains of monkeys up to 13 days after ic inoculation. No virus was detected in the central nervous system after 3 to 4 weeks, the longest postinoculation period examined. It was concluded that the subacute sclerosing panencephalitis-derived virus either lost its neurotropic properties at the passage level at which it was used or that it submerged into a silent stage and escaped detection. Neither immunosuppression nor concomitant autoimmune encephalitis had an effect on the survival of measles virus in the central nervous system. The histology of the nervous tissue was basically normal except for characteristic lesions of experimental allergic encephalitis in animals receiving the respective treatment.

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PubMed1967

Rapid identification of tick-borne encephalitis virus by the fluorescent antibody technique.

An immunofluorescence technique in tissue cultures and in mice, developed to provide a rapid means of identification of group B arboviruses, has been used to identify the tick-borne encephalitis virus. Identification in mouse-brain tissue was not possible when mice with vague clinical symptoms were used. Identification in chick-embryo-cell cultures was less time-consuming and laborious than identification in mouse brain, but more care was required, since only a small percentage of cells showed fluorescence. Identification can be accomplished in a few hours if mice in agony or cell cultures three to five days after inoculation are used.

Animals↗
PubMedDate not supplied

Standardization of poliovirus neutralizing antibody tests.

Recently the Forum for Advancement of Immunization Research sponsored a Collaborative Study on Poliovirus Antibody Titration. Twenty laboratories from 12 countries participated. There were considerable differences in detail of test performance and test results among laboratories. The sensitivity of the tests varied over a 10-fold range (geometric mean titer from 21 to 288). The average coefficient of variation ranged from 4.5% to 13.5%. Tests performed at the Food and Drug Administration indicated that Hep-2 cells were more suitable than Vero cells for poliovirus titration by cytopathic effect end point or plaque assay. However, the cell lines were equally suitable for polio antibody titration by neutralization of cytopathic effect. A high degree of sensitivity and optimal reproducibility of antibody assay were observed in tests utilizing a medium to low dose of virus and serum-virus incubation overnight at 36 C. On the basis of current experience, a protocol is proposed for a test that provides high sensitivity and reproducibility and is practical for large-scale determinations of poliovirus antibody.

Animals↗