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Biomedical subjects

P A Vanrolleghem

Publications and source records attributed to P A Vanrolleghem.

At least 19 recordsLinked to original sources

Role of yeast proliferation in the quality degradation of strawberries during refrigerated storage.

Quality changes of strawberries during storage can be caused both by microbiological and physiological processes. There is little known about the possible contribution of microbiological processes to the quality degradation of strawberries. In this study, quality of strawberries during storage was evaluated by analytical and sensorial analyses. It was the aim to investigate the influence of microbiological activity on the changes of different quality factors of strawberries during storage. During storage at 7 degrees C, quality was mainly determined by the odor and by visual defects. Regarding the odor, highly microbiologically contaminated late-season strawberries packaged in air at 7 degrees C became sensorially unacceptable due to the presence of high amounts of ethyl acetate. This could be attributed to the yeast proliferation: at yeast concentrations above 5.0 log cfu/g, an increase in ethanol was detected in the headspace of the strawberries. It was shown that ethanol was converted to ethyl acetate by strawberries resulting in an unacceptable odor. In an experiment with low microbiologically contaminated early-season strawberries, not reaching the above mentioned yeast counts, less ethyl acetate was detected which resulted in strawberries that were sensorially acceptable during the whole storage period (12 days). Strawberries packaged in modified atmosphere conditions showed a different quality pattern due to the effect of decreased O2-concentrations on both microbiological and physiological processes. This paper demonstrates that also microbiological processes on strawberries should be considered as they could play an important role in the sensorial quality when interacting with physiological processes.

Acetates↗

Spatio-temporal statistical models for river monitoring networks.

When introducing new wastewater treatment plants (WWTP), investors and policy makers often want to know if there indeed is a beneficial effect of the installation of a WWTP on the river water quality. Such an effect can be established in time as well as in space. Since both temporal and spatial components affect the output of a monitoring network, their dependence structure has to be modelled. River water quality data typically come from a river monitoring network for which the spatial dependence structure is unidirectional. Thus the traditional spatio-temporal models are not appropriate, as they cannot take advantage of this directional information. In this paper, a state-space model is presented in which the spatial dependence of the state variable is represented by a directed acyclic graph, and the temporal dependence by a first-order autoregressive process. The state-space model is extended with a linear model for the mean to estimate the effect of the activation of a WWTP on the dissolved oxygen concentration downstream.

Data Collection↗

Designing and performing experiments for model calibration using an automated iterative procedure.

Optimal experimental design for parameter estimation involves complex mathematical and practical steps in order to obtain a model with sufficiently accurate parameters. This paper proposes a methodology where user interaction is only required at the beginning of the experimental design procedure. All subsequent steps are carried out automatically, including: (1) finding the optimal experiment, (2) performing the experiments in practice, and (3) recalibrating the model. A software extension to an existing modelling and simulation package which performs this automatic procedure is also presented. Finally an illustration for the calibration of a one-step nitrification model using respirometric data is given. In this case, the application of the proposed procedure resulted, after three iterations, in considerably better confidence intervals on the parameter estimates, within the desired boundaries.

Automation↗

Towards a benchmark simulation model for plant-wide control strategy performance evaluation of WWTPs.

The COST/IWA benchmark simulation model has been available for seven years. Its primary purpose has been to create a platform for control strategy benchmarking of activated sludge processes. The fact that the benchmark has resulted in more than 100 publications, not only in Europe but also worldwide, demonstrates the interest in such a tool within the research community In this paper, an extension of the benchmark simulation model no 1 (BSM1) is proposed. This extension aims at facilitating control strategy development and performance evaluation at a plant-wide level and, consequently, includes both pre-treatment of wastewater as well as the processes describing sludge treatment. The motivation for the extension is the increasing interest and need to operate and control wastewater treatment systems not only at an individual process level but also on a plant-wide basis. To facilitate the changes, the evaluation period has been extended to one year. A prolonged evaluation period allows for long-term control strategies to be assessed and enables the use of control handles that cannot be evaluated in a realistic fashion in the one-week BSM1 evaluation period. In the paper, the extended plant layout is proposed and the new suggested process models are described briefly. Models for influent file design, the benchmarking procedure and the evaluation criteria are also discussed. And finally, some important remaining topics, for which consensus is required, are identified.

Benchmarking↗

Distributed virtual experiments in water quality management.

Since the complexity of virtual experiments (VEs) and their underlying models is constantly increasing, computational performance of monolithic software solutions is rapidly becoming insufficient. Examples of VEs are probabilistic design, model calibration, optimal experimental design and scenario analysis. In order to tackle this computational bottleneck, a framework for the distributed execution of VEs on a potentially heterogeneous pool of work nodes has been implemented. This framework was named WDVE (WEST distributed virtual experimentation) and is built on top of technologies such as C++, XML and SOAP. It was designed for stability, expandability, performance, platform-independence and ease of use. Complex VEs are most often composed of mutually independent sub-experiments, which can be run concurrently. With WDVE, a complex VE that is executed on a so-called Master machine will therefore attempt to execute its sub-experiments on Slave machines that have previously registered with the Master. The process of submitting requests for the execution of sub-experiments is transparent and involves the transfer of a description of the experiment to be executed, and the resources that are needed for the execution (i.e., model and input data). WDVE is in many ways similar to the Grid Computing paradigm, which is currently receiving widespread attention. However, WDVE is more geared towards application within the scope of water quality management.

Calibration↗

Controlling the nitrite:ammonium ratio in a SHARON reactor in view of its coupling with an Anammox process.

The combined SHARON-Anammox process for treating wastewater streams with high ammonia load is the focus of this paper. In particular, partial nitritation in the SHARON reactor should be performed to such an extent that a nitrite:ammonium ratio is generated which is optimal for full conversion in an Anammox process. In the simulation studies performed in this contribution, the nitrite:ammonium ratio produced in a SHARON process with fixed volume, as well as its effect on the subsequent Anammox process, is examined for realistic influent conditions and considering both direct and indirect pH effects on the SHARON process. Several possible operating modes for the SHARON reactor, differing in control strategies for O2, pH and the produced nitrite:ammonium ratio and based on regulating the air flow rate and/or acid/base addition, are systematically evaluated. The results are quantified through an operating cost index. Best results are obtained by means of cascade feedback control of the SHARON effluent nitrite:ammonium ratio through setting an O2 set-point that is tracked by adjusting the air flow rate, combined with single loop pH control through acid/base addition.

Air Movements↗

Application of a model-based optimisation methodology for nutrient removing SBRs leads to falsification of the model.

Recently, a model-based optimisation methodology for SBR operation has been developed and an optimal operation scenario proposed to improve N and P removal in a pilot-scale SBR. In this study, this optimal operation scenario was implemented and evaluated. The results of the implementation showed that the SBR performance was improved by approximately 50 and 40% for total nitrogen and phosphorous removal, respectively, which was better than predicted by the model. However, the long-term SBR performance was found to be unstable, particularly owing to settling problems developed after the implementation. When confronted with reality, the model used for the optimisation of the operation was found to be invalid. The model was unable to predict the nitrite build-up provoked by the optimal operation scenario. These results imply that changing the operation of an SBR system using a model may significantly change the behaviour of the system beyond the (unknown) application domain of the model. This is simply because the mechanistic models currently do not cover all the aspects of activated sludge systems, e.g. settling and adaptation of the microbial community. To further improve model-application practices, expert knowledge (not contained in the models) can be valuable and should be incorporated into model-based process optimisations.

Bioreactors↗

Model-based evaluation of an on-line control strategy for SBRs based on OUR and ORP measurements.

Application of control strategies for existing wastewater treatment technologies becomes necessary to meet ever-stricter effluent legislations and reduce the associated treatment costs. In the case of SBR technology, controlling the phase scheduling is one of the key aspects of SBR operation. In this study a calibrated mechanistic model based on the ASM1 was used to evaluate an on-line control strategy for the SBR phase-scheduling and compare it with the SBR's performance using no control strategy. To evaluate the performance, reference indices relating to the effluent quality, the required energy for aeration and the treated wastewater volume were used. The results showed that it is possible to maintain optimal SBR performance in the studied system at minimal costs by on-line control of the length of the aerobic and anoxic phases.

Bioreactors↗

Estimating costs and benefits of advanced control for wastewater treatment plants--the MAgIC methodology.

This paper discusses a methodology to estimate the costs and benefits of advanced control for wastewater treatment plants. The methodology has been applied to four wastewater treatment plants, representing four standard types of plants built in Flanders, Belgium. The paper outlines the methodology and illustrated results from one of the four design cases. General results are shown and contrasted with full-scale experience. The methodology appears to give realistic results and will be used for further refinement of default control algorithms for certain types of plants. A preliminary analysis indicates that on-line control can become cost-effective for plant sizes above 50,000 population equivalents.

Automation↗

New measurement techniques for secondary settlers: a review.

In activated sludge wastewater treatment research, most of the attention has been devoted to the biodegradation process. However, in terms of effluent quality, the final settling and clarification processes are at least as important because any particle carried over the overflow weir brings with it not only COD, but also nitrogen and phosphorus. In recent years we see indeed increased attention on this separation step, and alternatives such as membrane separation are increasingly introduced. Still, a large installed base of settlers exists, whose operation could/should be optimised. The measurement techniques presented in this contribution each focus on one of the key processes in the secondary settler: hydrodynamics, particle aggregation and breakage, hindered settling and compression. For each technique, the measuring principle is explained and a typical data set shown.

Flocculation↗

Sensor validation and reconciliation for a partial nitrification process.

Wastewater treatment plants (WWTP) are notorious for poor data quality and sensor reliability due to the hostile environment in which the measurement equipment has to function. In this paper, a structured residual approach with maximum sensitivity (SRAMS) based on the redundancy of the measurements is used to detect, identify and reconstruct single and multiple sensor faults in a single reactor for high activity ammonia removal over nitrite (SHARON) process. SRAMS is based on inferences, which are insensitive to the faults in the sensor of interest and sensitive to faults in the other sensors. It is used for four types of sensor failure detection: bias, drift, complete failure and precision degradation. The application of sensor validation shows that single and multiple sensor faults can be detected and that the fault magnitude and fault type can be estimated by the reconstruction scheme. This sensor validation method is not limited by the type or application of the considered sensors. The methodology can thus easily be applied for sensor surveillance of other continuously measuring sensors and analysers.

Bioreactors↗

Titrimetric monitoring of a completely autotrophic nitrogen removal process.

Fully autotrophic nitrogen removal processes, such as the combined SHARON-Anammox process, help to improve the sustainability of wastewater treatment. Successful operation of such a completely autotrophic system is, among others, based on the strict control of the SHARON reactor in order to produce an Anammox-suited influent with a 1:1 ammonium:nitrite ratio. The high quality and high frequency measurements provided by a titrimetric set-up measuring the total ammonium (TAN) and total nitrite (TNO2) concentrations facilitate this control considerably. In this study, the use of a titrimetric set-up for monitoring the combined SHARON-Anammox process is investigated. The technique that interprets on-line collected titration curves was applied to a lab-scale system. Comparison with classic colorimetric results gave statistically indistinguishable results for TAN and TNO2 concentrations in the SHARON reactor. In the Anammox reactor, only TAN could be determined by the investigated method due to the very low TNO2 concentrations. Phosphate, a potential inhibitor of the Anammox process, is available as an additional measurement in the effluent of the SHARON reactor. Three measurements are thus combined in one single instrument. The proposed measuring technique holds different advantages over the other TAN and TNO2 measurement techniques such as on-site availability, easy automation, the absence of the need for high dilutions and cost reduction.

Bioreactors↗

Effect of different river water quality model concepts used for river basin management decisions.

n this research the applicability of two different water quality concepts, a QUAL2E-based and a RWQM1-based water quality model is evaluated in terms of management decisions. The Dender river in Belgium serves as a case study for the application of the methodology. By using sensitivity analysis on both model concepts the important processes are revealed. Further, the differences between the predictions for a future scenario are analysed. The scenario chosen here is a reduction in fertiliser use of 90%, which reduces the diffuse pollution. This way, the advantages or disadvantages of using one concept against the other for this scenario are formulated. It was found that the QUAL-based models are more focussing on algae processes while the RWQM1 also takes into account processes in the sediment. Further the QUAL-based models are easier to calibrate, especially when only a small amount of data is available. Both concepts lead to more or less the same conclusions. However for some periods the differences become important and to reduce the uncertainty in those periods, more efforts should be spent in calibration and in better detection of parameters concerning sediment processes and diffusion.

Belgium↗

The CatchMod toolbox: easy and guided access to ICT tools for Water Framework Directive implementation.

Web-based toolboxes are handy tools to inform experienced users of existing software in their disciplines. However, for the implementation of the Water Framework Directive, a much more diverse public (water managers, consultancy firms, scientists, etc.) will ask for a very wide diversity of Information and Communication Technology (ICT) tools. It is obvious that the users of a web-based ICT-toolbox providing all this will not be experts in all of the disciplines and that a toolbox for ICT tools for Water Framework Directive implementation should thus go beyond just making interesting web-links. To deal with this issue, expert knowledge is brought to the users through the incorporation of visitor-geared guidance (materials) in the Harmoni-CA toolbox. Small workshops of expert teams were organized to deliver documents explaining why the tools are important, when they are required and what activity they support/perform, as well as a categorization of the multitude of available tools. An integration of this information in the web-based toolbox helps the users to browse through a toolbox containing tools, reports, guidance documents and interesting links. The Harmoni-CA toolbox thus provides not only a virtual toolbox, but incorporates a virtual expert as well.

Conservation of Natural Resources↗

Evaluation of the impacts of model-based operation of SBRs on activated sludge microbial community.

Impact of model-based operation of nutrient removing SBRs on the stability of activated sludge population was studied in this contribution. The optimal operation scenario found by the systematic model-based optimisation protocol of Sin et al. (Wat. Sci. Tech., 2004, 50(10), 97-105) was applied to a pilot-scale SBR and observed to considerably improve the nutrient removal efficiency in the system. Further, the process dynamics was observed to change under the optimal operation scenario, e.g. the nitrite route prevailed and also filamentous bulking was provoked in the SBR system. At the microbial community level as monitored by DGGE, a transient shift was observed to gradually take place parallel to the shift into the optimal operation scenario. This implies that the model-based optimisation of a nutrient removing SBR causes changes at the microbial community level. This opens future perspectives to incorporate the valuable information from the molecular monitoring of activated sludge into the model-based optimisation methodologies. In this way, it is expected that model-based optimisation approaches will better cover complex and dynamic aspects of activated sludge systems.

Bacteria↗

Evolution of an ASM2d-like model structure due to operational changes of an SBR process.

To model biological nitrogen and phosphorus removal systems with an affordable complexity, the ASM2d model structure is based on many assumptions. In this study, some of these assumptions, however, were observed to become invalid when the biological behaviour in the system altered in response to changes in the operation of the system, a pilot-scale N and P removing SBR. Particularly, the three applied operational scenarios resulted in three distinctive responses in the SBR, namely pronounced limitation of the hydrolysis of the organic nitrogen, nitrite build-up during aerobic conditions and also nitrite build-up during anoxic conditions. This shows that even for the same system with the same influent wastewater composition, the model structure of the ASM2d does not remain constant but adapts parallel to dynamic changes in the activated sludge community. On the other hand, the three calibrated ASM2d models still lacked the ability to entirely describe the observed dynamics particularly those dealing with the phosphorus dynamics and hydrolysis. Understanding the underlying reasons of this discrepancy is a challenging task, which is expected to improve the modelling of bio-P removing activated sludge systems.

Bioreactors↗

Modelling anaerobic digestion acclimatisation to a biodegradable toxicant: application to cyanide.

The observed acclimatisation to biodegradable toxicants in anaerobic cassava wastewater treatment is explained by modelling anaerobic cyanide degradation. A complete degradation pathway is proposed for cyanide. Cyanide degradation is modelled as enzymatic hydrolysis to formate and ammonia. Ammonia is added to the inorganic nitrogen content of the digester while formate is degraded by the hydrogenotrophic methanogens. Cyanide irreversible enzyme inhibition is modelled as an inhibition factor to acetate uptake processes. Cyanide irreversible toxicity is modelled as a decay factor to the acetate degraders. Cyanide as well as added phosphorus buffer solution were considered in the chemical equilibrium calculations of pH. The observed reversible effect after acclimatisation of sludge is modelled by a population shift between two aceticlastic methanogens that have different tolerance to cyanide toxicity. The proposed pathway is added to the IWA Anaerobic Digestion Model no.1 (ADM1). The ADM1 model with the designed extension is validated by an experiment using three lab-scale upflow anaerobic sludge bed reactors which were exposed to different cyanide loadings.

Anaerobiosis↗

Assessment of WWTP design and upgrade options: balancing costs and risks of standards' exceedance.

Numerical models can be used to evaluate design and upgrade scenarios of urban wastewater systems on the basis of their ecological consequences. The objective of this paper is to illustrate a systematic procedure of system design/upgrade. This procedure consists of the following steps: (1) data collection and data reconstruction; (2) model building and calibration; (3) evaluation of scenarios; (4) uncertainty assessment. In contrast to conventional practice, this approach allows to choose the most appropriate trade-off between cost of measures and risk of non-compliance with regulatory limits. An example of its application dealing with the assessment of WWTP design and upgrade options is provided. Results show that by reducing the tank volumes compared to conventional design procedures, costs can be reduced sensibly while the risk of not meeting legislative requirements are only slightly increased.

Cities↗