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Biomedical subjects

P A Karmanov

Publications and source records attributed to P A Karmanov.

9 recordsLinked to original sources
PubMed1983

[Characteristic effects of rabbit liver extract on hepatocyte proliferation in the regenerating liver of mice of various ages].

Chalone isolated by the Verly method was injected into animals of different age (2 groups) under partial hepatectomy. The percentage of hepatocytes in S-, G2-, M- and post-M-periods was determined by morphoautoradiography. These parameters were used for the reconstruction of the extent and dynamics of cell proliferation during 36-48 hours of regeneration. The suppressing effect of chalone on hepatocyte proliferation was more pronounced in young mice. The lack of complete inhibition of DNA synthesis and of the effect of proliferative synchronization was observed in both groups of mice.

Aging↗
PubMedDate not supplied

[Characteristics of messenger RNA for interferon and of interferon-inducing RNA isolated from Newcastle disease virus infected cells].

Two kinds of RNA capable of producing interferon in a heterologous cell system occur in the cells producing interferon in response to induction by Newcastle disease virus. One of them has messenger activity for interferon and is presented by a single-stranded structure with sedimentation constant of 10-22 S. This RNA appears to belong to cellular messenger RNA. The other is capable of inducing interferon production by heterogenous cells, resistant to treatment with RN-ase and has sedimentation constant greater than 35 S. In our opinion, this RNA is the primary replicative RNA of the virus-inducer.

Culture Techniques↗
PubMedDate not supplied

[The nature of the lengthening of the G2 phase in the mitotic cycle of parenchymal cells of the mouse liver].

The phenomenon of G2 phase prolongation was found in the population of mouse hepatocytes. In normal postnatal liver growth, G2 phase prolongation in not pronounced and occurs in a small fraction of proliferating hepatocytes. In case of liver regeneration after removal of 2/3 of the organ, G2 phase prolongation is observed in a population of hepatocytes, which response to the proliferative stimulus first. Estimation of individual variation in expression of prolonged G2 phase along with the detailed analysis of the structure of the process of proliferation in the main population of hepatocytes (cells with normal G2 phase) allows to define the biological meaning of the "G2-population" observed. The prolongation of G2 phase may result from non-specific cell damage in mitotic cycles, caused by destruction of trophic relations in liver during its growth and regeneration.

Aging↗
PubMedDate not supplied

[Analysis of the prereplicative period of liver regeneration in young mice. The fate of the hepatocytes in the DNA synthesis phase at the moment of surgery].

In the liver of young mice after partial hepatectomy the hepatocytes which entered S-phase prior to the operation complete DNA synthesis in time and their major part is arrested in G2-phase. During the second half of lag-period (12-24 h after the operation) about half of these cells enters mitosis. Another half of the hepatocytes which were in S-phase during the operation does not proceed to mitosis. The structure of the nuclei of these latter suggests the regression of premitotic preparation: some nuclei return to the initial G0-structure of the normal heterosynthetic hepatocyte (endoreproduction cycle) and others preserve the premitotic structure characteristic of S-phase. A number of morphological features approaches this latter subpopulation to S-nuclei of tumour cells of the mouse hepatoma No. 46. A suggestion is put forward that this subpopulation has lost its capacity for heterosythesis due to operation liver trauma.

Animals↗