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Biomedical subjects

O Wolf

Publications and source records attributed to O Wolf.

At least 37 records · Page 2Linked to original sources
PubMed1990

Investigations of the antiatherosclerotic effect of the thromboxane A2 receptor antagonist Daltroban.

In the pathogenesis of atherosclerosis an accumulation of lipids, predominantly in the form of cholesteryl esters within the blood vessel wall is observed. Interaction of the plaques, e.g. with platelets, is suggested to contribute considerably to their growth. Compounds affecting both processes should prevent atherosclerotic progression and therefore are of great interest for potential therapeutic use. We investigated the effect of Daltroban, which has been characterized as a selective thromboxane receptor antagonist with platelet inhibiting activity, in two models: 1) cholesterol metabolism in liver cells and 2) progression of atherosclerosis in aorta of hypercholesterolemic rabbits. Daltroban reduced 14C-acetate incorporation into cholesteryl esters stronger than into cholesterol in rat hepatocyte monolayer cultures. In male white New Zealand rabbits fed 0.5% cholesterol enriched diet for 96 days, coadministration of Daltroban beginning at the 42th day on diet reduced aortic cholesterol content, plaque covered surface of aortic wall, and lumen stenosis by plaques more than 30 percent. The significant inhibition of the progression of atherosclerosis in the hypercholesterolemic rabbits by Daltroban is suggested to be effected by both inhibition of cholesterol metabolism as well as of platelets.

Animals↗
PubMed1990

[Results of the analysis of a regional noise-exposure outpatient unit].

An evaluation of our regional register of noise-exposed persons comprehended within the framework of medical ability and control examinations of workers was given here. On the occasion of medical ability examinations of workers at the beginning of their employment 448 persons were examined. 49 of them had to undergo an additional ENT investigation because working under noise conditions in future. 5.4 p.c. of those were not passed as fit for working in noise environment. On the occasion of medical control examinations of workers during their employment 1893 persons were examined now. 184 of them had to undergo an additional ENT investigation because of their exposition to noise. Following that, it was necessary for 1.4 p.c. of the latter to change their working place, but for 0.6 p.c. of them an announcement to suspicion of occupational hearing damage must be made. In connection with the data above mentioned great attention must be payed to early ear diseases on the one hand, and to hearing damages existing before on the other hand.

Adult↗
PubMed1987

Expression of the gene for the beta subunit of mouse thyrotropin results in multiple mRNAs differing in their 5'-untranslated regions.

The gene encoding the beta subunit of mouse thyrotropin (TSH beta) has been isolated from a mouse genomic library, and its nucleotide sequence has been determined. Blot hybridization analysis of restriction enzyme digests of mouse DNA indicates that there is a single mouse TSH beta gene. The gene is 4.8 kilobases in length and contains five exons, which are 27, 47, 41, 163, and 328 base pairs long. Exons 1, 2, and 3 encode only 5'-untranslated mRNA sequences and are separated by introns that are 150 and 380 base pairs long. The protein-coding mRNA sequences are found in exons 4 and 5 and are interrupted by a 460-base pair intron. The position of this intron, between the codons for amino acids 34 and 35, has been conserved in all the known glycoprotein hormone beta subunit genes. Exons 3 and 4 are separated by a large 3.2-kilobase intron. When primer extension analysis, using an oligonucleotide primer complementary to exon 4 sequences, was employed to locate the transcription start site, four products were obtained. Nucleotide sequencing of these products showed that they were derived from separate TSH beta mRNAs that differed in the lengths of their 5'-untranslated regions. These 5'-untranslated mRNA sequences are derived from different combinations of exons 1, 2, and 3, each spliced to exons 4 and 5. The longest 5'-untranslated sequence, 116 nucleotides long, includes exons 1, 2, and 3 and the first base of exon 4; the shorter 5'-untranslated regions, 75, 69, and 28 nucleotides long, arise by splicing out the second and/or the third exon sequences. In contrast to the mouse TSH beta gene, transcription of the rat TSH beta gene from the analogous start site has been reported to give only a single mRNA, with a 5'-untranslated region of 28 nucleotides. Divergence of the mouse and rat TSH beta gene sequences at RNA splice sites can account for the absence of exon 2, but not exon 3, sequences in rat TSH beta mRNA. Primer extension and RNase protection analyses also showed that the mouse TSH beta gene contains a second transcription start site, located 43 base pairs upstream of the first start site, in a position corresponding to that in the rat TSH beta gene. Each start site in the mouse gene is flanked by characteristic TATAA box and CAAT box sequences.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗
PubMed1986

[111Indium-thrombocyte scintigraphy and percutaneous transluminal dilatation (angioplasty) of supra-aortic vascular stenoses. A new method for deciding therapy and for follow-up control].

Scintigraphy with 111indium-marked autologous platelets has been used during percutaneous transluminal dilatation of supra-aortic arterial stenoses; there were 14 successful dilatations and one attempted dilatation. The material consisted of nine stenoses of the internal carotid artery, two stenoses at the origin of the vertebral artery and four stenoses of the first part of the left subclavian artery. Deposition of thrombi could be demonstrated by scintigraphy, both before and after catheter dilatation. The value of this method for deciding on the type of treatment and for observing the course of percutaneous dilatation of cerebral vessels is discussed.

Angioplasty, Balloon↗
PubMed1981

[Considerations on the etiology of pancreatitis].

Among numerous factors which induce or exacerbate pancreatitis infection of the biliary-tract--with or without lithiasis--is important. In suitable cases intervallic restoration of this tract is the best way to avoid recidivation. From a total of 624 patients in our department with cholepancreatitis in 225 cases this therapy was performed. After a recidivation of pancreatitis which required hospitalisation within at least 3 years was to be seen in 4.7%.

Alcohol Drinking↗