lambda SK9--a diphasmid for constructing genomic libraries, capable of converting inserts into plasmid and single stranded forms.
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Bacteriophage lambda↗
Biomedical subjects
O V Turina
Publications and source records attributed to O V Turina.
SK18 diphasmid for constructing genomic libraries with blue/white selection, multiple cloning sites, and with the M13 and lambda ori.
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Base Sequence↗
Lambda SK3 and lambda SK5, vectors for constructing genomic libraries derived from lambda EMBL3 and lambda EMBL4 by insertion of lac gene.
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Bacteriophage lambda↗
Rapid isolation of lambda phage DNA in micro- and macro-variants.
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Bacteriophage lambda↗
[Rapid isolation of phage lambda DNA].
A modified procedure in two versions (micro, for 10 ml of phage lysate, and macro, for 200-500 ml) is described for preparing lambda phage DNA. The main advantage of the modified method is that it gives a possibility to isolate high-quality DNA from lambda phage lysates in 2-3 hrs. Only standard solutions (TE, NaCl, SDS, MgCl2, EDTA, RNAse A) were used throughout the whole protocol. Incubation with DNAse I and proteinase K was omitted and in microvariant concentration of the phage by PEG 6000 was excluded. Digestion by RNAse A was performed in solution with EDTA and SDS and leads to RNA degradation. The yields of DNA (0.5-2 micrograms per ml of L-broth) are similar to those obtained by other methods. DNA quality is better than in the samples of DNA prepared by other express-methods and practically the same as after CsCl centrifugation. DNA can be used for splitting by restriction enzymes, cloning and gene library construction.
Bacteriophage lambda↗