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Biomedical subjects

O Tulunay

Publications and source records attributed to O Tulunay.

31 records · Page 2Linked to original sources
PubMed1997

Immunohistochemical determination of p53 protein in prostatic cancer and prostatic intraepithelial neoplasms.

A role of genetic alterations in the p53 tumor suppressor gene has been implicated in many types of human malignancies. In this study, we examined the prevalence of immunohistochemically detectable p53 accumulation in prostatic tissues obtained from patients with prostatic adenocarcinoma, benign prostate hyperplasia and prostatic intraepithelial neoplasms. Six of 36 (16.7%) cancer cases and 2 of 11 (18.2%) cases of high-grade prostatic intraepithelial neoplasms showed p53 expression while no nuclear staining was observed in normal and hyperplastic prostatic tissues. Correlation of p53 expression with cancer stage, Gleason score and serum prostate-specific antigen level demonstrated that there was no statistically significant relationship between p53 expression and these clinicopathological parameters. Also, no significant association between p53 expression and prognosis was observed.

Adenocarcinoma↗
PubMed1997

Carcinosarcoma of the prostate. A case report and a possible evidence on the role of hormonal therapy.

A case of prostatic carcinosarcoma is presented with histopathologic and immunohistochemical characteristics. A 70-year-old man presented with a history of anti-androgen (cyproterone acetate) therapy for prostatic adenocarcinoma. Diffuse and strong staining for progesterone receptor was observed in the carcinosarcoma specimen although it was completely negative in the previous adenocarcinoma specimen. It may be speculated that hormonal therapy might have facilitated the selection of a progesterone-dependent subclone of tumor cells with the ability of mesenchymal differentiation and that genetic instability due to p53 inactivation might have played a role in this process.

Adenocarcinoma↗
PubMed1984

Membranous nephropathy: predictors of terminal renal failure.

By univariate analysis of patients with membraneous nephropathy, terminal renal failure was associated with male sex, a large amount of proteinuria, low serum albumin concentration, low creatinine clearance rate, high serum creatinine concentration, and high systolic blood pressure, but was not associated with age or prednisone treatment. In a multivariate life table analysis that controlled for all these factors simultaneously, the risk of developing terminal renal failure was significantly independently associated only with sex, serum albumin concentration, and prednisone treatment, being higher in men, lower in those treated with prednisone, and inversely related to serum albumin. Except for the minimal electron-dense deposition, the electron microscopic findings had no predictive value.

Adolescent↗
PubMed1982

Immunofluorescent staining of plastic-embedded renal tissue.

Human renal tissues were fixed in cold 4% paraformaldehyde and 0.2% picric acid, embedded in glycol methacrylate (GM), and sectioned at a thickness of 1 to 8 micrograms. Sections were incubated for three hours with nonspecific protease, stained with fluorescein isothiocyanate-conjugated goat antihuman serum samples, and examined by immunofluorescent microscopy. The immunofluorescent staining was compared with that in cryostat sections of the same tissues prepared and stained by conventional methods. The GM-embedded sections showed consistent staining of antigens, comparable with that in the corresponding cryostat sections, and better preservation of tissue structure than in cryostat sections. The antigens in tissues processed by this method are probably stable for long periods.

Antigens↗
PubMed1978

Experimental amyloidosis in mice of different ages. Effects of neonatal thymectomy and amyloidogenic stimulation of pregnant mice.

Murine amyloidosis, induced by repeated injections of sodium caseinate, was compared in young and adult Swiss albino mice and in young thymectomized and nonthymectomized mice. Thymectomized mice developed amyloidosis earlier and more severely than intact mice. Mothers of young mice were injected with sodium caseinate during pregnancy and after birth sodium caseinate injections were given to the offspring, but this treatment did not seem to induce amyloidosis in the young mice. A much shorter latent period before development of amyloidosis was seen in the adult group than in the young mice. This may be the result of a depletion of cellular and humoral immunity with aging. The shorter latent period and more severe development of amyloidosis in the thymectomized groups supports the view that an impaired immunological state may constitute a basis for the development of amyloidosis.

Aging↗
PubMed1975

Protection of lethally irradiated mice with allogeneic fetal liver cells: influence of irradiation dose on immunologic reconstitution.

After lethal irradiation long-lived, immunologically vigorous C3Hf mice were produced by treatment with syngeneic fetal liver cells or syngeneic newborn or adult spleen cells. Treatment of lethally irradiated mice with syngeneic or allogeneic newborn thymus cells or allogeneic newborn or adult spleen cells regularly led to fatal secondary disease or graft-versus-host reactions. Treatment of the lethally irradiated mice with fetal liver cells regularly yielded long-lived, immunologically vigorous chimeras. The introduction of the fetal liver cells into the irradiated mice appeared to be followed by development of immunological tolerance of the donor cells. The findings suggest that T-cells at an early stage of differentiation are more susceptible to tolerance induction than are T-lymphocytes at later stages of differentiation. These investigations turned up a perplexing paradox which suggests that high doses of irradiation may injure the thymic stroma, rendering it less capable of supporting certain T-cell populations in the peripheral lymphoid tissue. Alternatively, the higher and not the lower dose of irradiation may have eliminated a host cell not readily derived from fetal liver precursors which represents an important helper cell in certain cell-mediated immune functions, e.g., graft-versus-host reactions, but which is not important in others, e.g., allograft rejections. The higher dose of lethal irradiation did not permit development or maintenance of a population of spleen cells that could initiate graft-versus-host reactions but did permit the development of a population of donor cells capable of achieving vigorous allograft rejection. These observations contribute to understanding of some of the persisting immunodeficiencies that are observed in man after fatal irradiation and bone marrow transplantation. These results should suggest better approaches to more effective cellular engineering for correction of immunodeficiency diseases and for treatment of immunodeficiency diseases and of leukemias and malignancies of man.

Animals↗
PubMedDate not supplied

Expression of p53 protein in pterygium.

PURPOSE: The pathogenesis of pterygium is still not completely understood and many environmental factors, including ultraviolet (UV) radiation, play an important role in its etiology. Chronic exposure to UV radiation causes mutations in the p53 tumor suppressor gene, eventually leading to tumor formation. We analyzed the immunohistochemical expression of p53 proteins in pterygial tissues to determine the role of the p53 tumor suppressor gene in the development of pterygium. METHODS: Pterygial specimens were studied immunohistochemically using antibodies against p53 protein. RESULTS: Out of 38 specimens studied, 35 (92.1%) had conjunctival epithelial cells without p53 specific nuclear staining. Only three specimens (7.9%) had a few p53 stained cells. The role of UV radiation in the pathogenesis of pterygium is supported by epidemiological, geographical and microscopic findings. However, our results are not consistent with these data on a genetic basis. CONCLUSIONS: We conclude that defective p53 tumor suppressor gene function seems to have no role in the pathogenesis of pterygium.

Adult↗