Reversed-phase high-performance liquid chromatographic separation of the tetrameric lectin concanavalin A from Canavalia ensiformis.
Explore the source record for details and available documents.
Biomedical subjects
Publications and source records attributed to O Sticher.
Explore the source record for details and available documents.
An improved procedure for the direct determination of l-dehydroascorbic acid (DHAA), in addition to l-ascorbic acid (AA), has been developed. The two biologically active forms of vitamin C were separated using reversed-phase high-performance liquid chromatography. DHAA was reduced to AA with dithiothreitol (DTT) in a post-column reaction system. Complete conversion was achieved at 50 degrees C using a 1-ml reaction coil. Recoveries were in the range of 95-99% and both forms could be detected spectrophotometrically at 267 nm with high sensitivity. Reproducible results [relative standard deviations 2.4% (DHAA) and 1.0% (AA), n = 5] were obtained when the method was applied to the analysis of rose hip samples.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
From a sample of the red alga Plocamium cartilagineum, four polyhalogenated monoterpenes [1-4] were isolated and characterized with (3R*, 4R*)-1-bromo-7-chloromethyl-3,4-dichloro-3-methyl-1E,5E,7-octatrie ne [1] being a new compound. Also reported are antifungal, antibacterial, and molluscicidal activities, as well as the brine shrimp toxicity of these metabolites.
A new iridoid glucoside, 8-O-acetylshanzhiside (1), was isolated from the aerial parts of Phlomis tuberosa, together with two known iridoid glucosides, shanzhiside methyl ester and lamalbide. The known phenylethanoid glycosides acteoside and forsythoside B were also obtained and characterized. The structure of 1 was determined by means of 1D- and 2D-NMR spectroscopic evidence.