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Biomedical subjects

O Rozhold

Publications and source records attributed to O Rozhold.

At least 19 recordsLinked to original sources
PubMed1991

Histological and biochemical characteristics of kryoprotected embryonic nervous tissue for transplantation purpose.

In clinical and experimental conditions, a biochemical equipment and a histological image of a fresh embryonic nervous tissue and a tissue conserved by deep freezing for transplantation purposes were examined. By repeated examination of neurotransmitters, the contents of dopamine and serotonin were found unchanged by freezing. In a human embryo, serotonin predominates over dopamine, while in chicken embryos, used for xeno-transplantations, it is vice versa. As for the microscopic structure of nervous tissue, no influence of kryopreservation for 4 to 14 days was found.

Animals↗
PubMed1991

Correlation of histopathological and clinical findings in neuromuscular diseases.

Our file comprises bioptic and necroptic materials of skeletal muscles: progressive spinal muscle atrophy of adults 16, amyotrophic lateral sclerosis 12, infantile progressive spinal amyotrophy Werdnig-Hoffmann 9, chronic progressive amyotrophy Oppenheim 6, syringomyelia and syringobulbia 3, conditions after spinal cord accidents. Diagnosis was difficult because one time the neurological marks were at the head and other time the muscular ones. In the clinical picture there was muscle weakness and atrophy, the spontaneous and palpation painfulness was missing. Muscle weakness und its intensity did not correspond to the degree of muscle atrophies. The discrepancies of electromyographic and histopathologic pictures are explained by occurrence of so-called concomitant myopathies, which arise most probably from the disorders of histioceptive-proprioceptive system.

Electromyography↗
PubMed1991

A contribution to the problem of atypical polymyositis in children.

The diagnostics of polymyositis has frequent difficulties, first af all in atypical forms. These can be presented under different names, which lead clinicians to an erroneous way. For example, "climacteric myopathy" (Kaeser) is considered an endocrinous myopathy, "late onset myopathy" a disease joined with old age (as well as "Werner's disease") or "benign children myopathies" (Walton Natrass). In the last years, a distinctive clinical picture is, due to almost causal therapy by cytostatics and immunosuppressives, worn out and therefore these diseases are called "near states" or "overlap syndrome". In our patients, the correctness of the diagnose was confirmed, apart from electromyographic and histopathological findings, primarily by treatment response and the course of the disease.

Adult↗
PubMed1991

[Directed photonucleolysis of the intervertebral disk and its herniation. Preparation for percutaneous laser diskectomy].

Under model conditions it proved possible, using a whole-body stereotactic apparatus, to focus on a human intervertebral disc and its prolapses and eliminate them by photocoagulation, using a laser. The suitable cannulae have a 3 mm diameter and an output of 6 to 12 W. The necessary lesion is reached in a fractionated manner within 1 minute. Thus the conditions were created for the new technique of percutaneous disectomy which has been done hitherto by means of chemical substances or mechanically by incision and suction.

Humans↗
PubMed1990

Photonucleolysis of intervertebral disc and its herniation. Preparation to percutaneous laser discectomy.

Making use of a whole-body stereotactic device under model conditions a human intervertebral disc and its herniations could be targeted and removed with a laser by photocoagulation. The applicable cannulae are 3 mm in diameter and of a 6 to 12 W output power. The required effect is reached within one minute. The situation is now prepared for the clinical introduction of the new technique of discectomy, which is still performed with chemicals or mechanically section and suction.

Animals↗
PubMed1990

[Technics of preparation and collection of embryonal nerve tissue for transplantation in clinical practice].

The proper author's experience is used in discussing possibilities on how to obtain the embryonal nerve tissue's samples for transplantation in various CNS diseases. The most reliable, though a unique one is the obtention of the whole foetus. This may be exclusively done by the miniabortion using suction curettage, or eventually at greater dilatation of cervix with the use of fine abortive forceps providing the ultrasound control. The most secure source of tissue in order to make transplantation is the removal of pregnant uterus, e. g. for its myomatosis. The proper preparation of tissue for transplantation is provided with maximally aseptic conditions.

Abortion, Induced↗
PubMed1990

On the treatment of vertebrogenic pain using laser-induced lesion of the spinal ganglia.

Recently, there has been an increase in the use of spinal-ganglia lesions as a means of surgical treatment of postdiscotomic algic syndromes. After relapses following thermocoagulation, the authors began to use lesions caused by a laser beam of an output of 30 W and temperature of 2,000 degrees C. As the resulting changes are irreversible, lasting effects of the procedure can be expected.

Back Pain↗
PubMed1990

Special structure of the skeletal muscle musculus vocalis.

Histopathological examination of 12 vocal muscles reveals special structure of this muscle which the vocal folds are based upon. The bundles of the muscle fibres passing in two planes perpendicular to each other alternate with stripes of the interstitium, seromucinous glandules and connective tissue septa with elastic fibres separating the groups of cross-striated muscle fibres of various diameters. It represents a physiological variety of the skeletal muscle structure conditioned by its function.

Adult↗
PubMed1990

Histopathological evidence of immunological and pseudoimmunological reactions by transplantation of embryonic and foetal nervous tissue in experimental conditions.

The analysis of 11 unsuccessful transplantation of embryonal nervous tissue into recipient brain gives the possibility to distinguish the immunological rejection from the pseudoimmunological response, conditioned by simple tissue necrosis. It is necessary to calculate not only with the antigenous character of tissue, but also with the grow potency and the nourishment of the graft in the recipient brain.

Animals↗
PubMed1990

Histopathological changes in paravertebral muscles by chronic discopathies.

Samples of paravertebral muscles were obtained from the lesion sites of forty patients operated on for recurrent lumboischiadic syndrome of discogenic etiology. None of the specimens was physiological. All of them were pathologically altered in various grades of involvement. The histopathological changes were highly varied. The findings included muscle fibre atrophy of a nonspecific type, neurogenic atrophy of typical fascicular distribution. Apart from atrophic fibres there were hypertrophic ones either at the same time or in connection with another histopathological process. Changes of myopathic nature were also present with rounded up muscle fibres and shift of nuclei from the subsarcolemnic spaces into the centre of the muscle fibre, multiplication of the connective tissue and vicarious growth of adipose tissue. The visible neuromuscular spindles contained thickened connective tissue capsules and atrophied intrafusal fibres. The present authors' conclusion of their interpretation of these histopathological changes is that they are not produced by one but by a whole set of factors. What results is a cyclic nature of the changes, when it is hard to decide whether we are facing a primary cause or its sequela. The regular company of discopathies are repeated microtraumas during recurrent paravertebral contractures and spinal blocks. A not negligible part can be played also by primary muscle diseases affecting, among others, back muscles, e.g. progressive muscular dystrophy of Duchenne's and Becker's type, various myopathies, nonspecific myositis and paravertebral polymyositis, and other rheumatological involvement. These muscular diseases with disturbed proprioception causing unphysiological posture and loading the spine can share in the origin degenerative processes on intervertebral disks.

Adult↗
PubMed1990

[Transplantation of embryonal nerve tissue into the brain of donors under experimental and clinical conditions].

The authors, stimulated by the results of transplantations of embryonic nervous tissue under experimental conditions and by findings on the biochemical background of some manifestations of mental diseases, dared to use for the first time transplantation of portions of human embryonic brain in the treatment of schizophrenia. Before the experiment they evaluated the bilateral xeno as well as autotransplantations in ten cats. Under clinical conditions they used transplantation of embryonic nervous tissue in two patients with the acute and chronic form of schizophrenia. The operation was performed, using Mundinger's stereotactic apparatus controlled by a computer. The tissue from the central portion of the embryonic brain from the 5th and 7th week of gestation with a high serotonin content was deposited bilaterally in the septal area in the recipient's brain. They recorded improvement in particular in a case of acute schizophrenia. By monitoring the foci of transplanted tissue by computed tomography and magnetic resonance, they provided evidence that embryonic nervous tissue is incorporated in the recipient's brain without reverting into malignant growth and that it is not rejected by the immune system of the recipient.

Adult↗
PubMed1990

[Perspectives in the treatment of some types of pain using lasers. Histological correlation with experimental results].

Treatment of some painful syndromes in the innervation area of spinal ganglia, particular in persisting pain after operations of prolapsed intervertebral discs or operations of the discs, call for new therapeutic methods. Promising possibilities are elimination of the spinal ganglion or nucleolysis of the disc by laser rays, as revealed by experimental studies in vitro and histological check-up of the affected tissue. Three concentric layers of the focus, the carbonization zone, the zones of evaporization and coagulation display irreversible changes which are prerequisites of a permanent effect.

Ganglia, Spinal↗
PubMed1989

Paravertebral polymyositis.

Pains in the back are usually of vertebrogenic etiology, though such discomfort can be caused by a primary muscular involvement as well - polymyositis of paravertebral muscles. Five patients were studied in the present investigation with this quite rare disease. Four of them had the diagnosis confirmed by histology, the last patient could not have the biopsy performed as she was already on a successful corticosteroid therapy. Histopathology of the muscles demonstrated a polymyositis of the parenchymatous type. Owing to a considerable polymorphism showed by the troubles and often also coincidence with the vertebrogenic component, the diagnosis and classification of primary chronic polymyositis face a series of difficulties. The disease becomes clinically manifested by muscle pain, both spontaneous and on palpation, atrophies and frequent muscle contractions. These symptoms can then result in disorders affecting the dynamics and statics of the spine. The possible participation of repeated microtraumas as one of the factors contributing to the origin of polymyositis is also discussed on the present study. The disease is treated in the same way as most of the other collagenoses, the primary chronic forms showing a much more favourable prognosis than the secondary chronic ones developing from acute and subacute forms.

Adult↗
PubMed1989

Thibierge-Weissenbach syndrome.

Systemic diseases of the connective tissues occur either separate or combined with other collagenoses. Sometimes it is hard to define the clinical unit exactly and therefore these states are called overlap syndromes or close syndromes. One of such collagenoses coinciding with these diseases is calcinosis. It is most often found in children but rarely in adults. The subject of the present study are problems of the diagnosis and the current possibilities of treating calcinosis.

Collagen Diseases↗
PubMed1989

Problems of taking embryonic nervous tissues for transplantation purposes.

Legal miniabortions were utilized for taking 106 samples of the uterine cavity contents from 83 women. The material consisted of 15 embryonic and 19 maternal tissues. The embryonic nervous tissue was discovered in 7.7% of the specimens examined. The suitability of obtaining samples for transplantation with the currently used technique of legal abortions is considered.

Abortion, Legal↗