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Biomedical subjects

O Rasmussen

Publications and source records attributed to O Rasmussen.

At least 55 records · Page 3Linked to original sources
PubMed1989

Preparatory studies for the use of plant protoplasts in space research.

An experiment using plant protoplasts has been accepted for the IML-1 mission to be flown on a space shuttle in 1991. Preparatory experiments include studies of cell wall formation, cell division, the effect of simulated weightlessness using fast and slow rotating clinostats, and the development and testing of hardware for the IML-1 mission. After 24 h at 25 degrees C, protoplasts isolated from hypocotyls or leaves of rapeseed seedlings, or from carrot suspension cells, show 60, 20 and 15% cell wall formation, respectively. The time course of formation of the cell wall and cell division could be delayed by treatment at low temperatures or immobilization in alginate or agarose. This aspect is of importance in connection with problems of late access to the space shuttle before launch. At 4 degrees C only 18% of the rapeseed hypocotyl protoplasts had formed cell walls after 24 h. Protoplasts immobilised in agarose or alginate gradually regain their cell division capacity and after 72 h the frequencies are 51 and 26%, respectively, compared to non-immobilised control protoplasts. A significant decrease in cell division activity is observed after rotation for 6 h on the slow clinostat. A similar effect is not observed on the fast clinostat. Protoplasts, cultured in the specially designed plant chamber for up to 14 days established cell aggregates which have further developed into plants.

Alginates↗
PubMed1988

Comparison of blood glucose and insulin responses in non-insulin dependent diabetic patients. Studies with spaghetti and potato taken alone and as part of a mixed meal.

Recently, we demonstrated that spaghetti caused significantly lower glycaemic response than rice and potato in insulin-dependent diabetic (IDDM) subjects and that this difference was also present when spaghetti and potato were taken as part of a mixed meal. We have now compared the blood glucose and insulin responses to 50 g of carbohydrate in the form of white bread, potato and white spaghetti in 6 non-insulin-dependent diabetic (NIDDM) patients. The blood glucose response after white spaghetti observed over a 3-h period was only 60 +/- 10 per cent (P less than 0.02) of that seen in response to potato (395 +/- 116 mmol/l x 180 min vs 641 +/- 108 mmol/l x 180 min) and 47 +/- 9 per cent (P less than 0.01) of that seen in response to white bread (395 +/- 116 mmol/l x 180 min vs 805 +/- 93 mmol/l x 180 min). Insulin responses showed an identical pattern reflecting the glycaemic responses. To see if the difference in the glucose responses in NIDDM patients is preserved if these carbohydrate-rich foods are taken as part of a mixed meal we looked at the blood glucose and insulin responses to 50 g of carbohydrate in the form of potato and white spaghetti when ingested together with bolognese sauce (167 g) in 7 NIDDM patients.(ABSTRACT TRUNCATED AT 250 WORDS)

Aged↗
PubMed1987

Early diagnosis of acute myocardial infarction with a rapid latex agglutination test for semi-quantitative estimation of serum myoglobin.

A rapid latex agglutination test for the detection of elevated levels of myoglobin in serum was evaluated in a prospective study of 236 patients consecutively admitted to a Coronary Care Unit on suspicion of acute myocardial infarction (AMI). The final diagnosis was made according to the WHO criteria. The prevalence of AMI was 0.45 with a male to female ratio of 2:1. In all patients at least two blood samples were collected with 4 hours interval 4-12 hours after the onset of symptoms. All sera were analysed for myoglobin by a latex agglutination test and by a radioimmunoassay (RIA). The latex test was performed twice, first as an emergency test by the technical assistant on duty and later by another well-trained technical assistant as her daily routine work. If the latex test was carried out each day by the same well-trained technical assistant, the test results agreed well with the RIA test results, and the false-negative fraction for patients with AMI constituted 0.06 and the false-positive fraction for patients without AMI 0.46. However, when the latex test was performed by the occasional technical assistant on duty, a relatively high degree of discrepancy was observed between the latex test results and the RIA test results, thus giving a false-negative fraction of 0.11 and a false-positive one of 0.36. In conclusion, performed under optimal laboratory conditions, the latex test can be used as a reliable method to estimate elevated levels of serum myoglobin. However, used as a bedside emergency examination, the test results correlated rather poorly to the RIA test results, and consequently the latex myoglobin test seems to be of minor clinical importance in the early evaluation of patients with suspected AMI.

Female↗
PubMed1986

Differential glycaemic effects of potato, rice and spaghetti in type 1 (insulin-dependent) diabetic patients at constant insulinaemia.

The blood glucose responses to cooked potato, rice and spaghetti were studied in six Type 1 (insulin-dependent) diabetic patients who had attained euglycaemia by the artificial pancreas prior to the meal intake. The amount of potato (raw weight 200 g), parboiled rice (raw weight 50 g), and spaghetti (raw weight 50 g) had approximately identical caloric content (range 203-225 kcal) and amount of available carbohydrate (range 39.4-43.4 g). The postprandial blood glucose response areas after cooked potato and cooked parboiled rice were similar (180 min values: cooked potato: 1190 +/- 110 mmol/l X min, cooked rice: 1160 +/- 140 mmol/l X min and 240 min values: cooked potato: 1690 +/- 140 mmol/l X min, cooked rice: 1740 +/- 210 mmol/l X min). In contrast, the response after cooked spaghetti was slower and less pronounced (180 min value: 830 +/- 80 mmol/l X min and 240 min value: 1320 +/- 120 mmol/l X min), and was significantly smaller than those of cooked potato (180 min: 2p less than 0.01 and 240 min: 2p less than 0.01) as well as cooked rice (180 min: 2p less than 0.01 and 240 min: 2p less than 0.02). Our study emphasizes the importance of determining the glycaemic response of foodstuffs under conditions of isoinsulinaemia.

Adult↗
PubMed1986

Early observations of S-myoglobin in the diagnosis of acute myocardial infarction. The influence of discrimination limit, analytical quality, patient's sex and prevalence of disease.

By means of a graphical method the influence of the analytical variation and the discrimination limit (DL) on the diagnostic power of the maximum serum myoglobin value observed from 4 to 12 h after onset of symptoms in 291 patients suspected for myocardial infarction (AMI) was examined. The prevalence of AMI was 0.45 and the male to female ratio 2:1. Serum myoglobin (S-myoglobin) was measured by a radioimmunoassay (RIA) with a coefficient of analytical variation (CVA) of 9%. For the distributions of the log values of maximum S-myoglobin for AMI patients and non-AMI patients straight lines were obtained on a probit scale. A statistically significant difference was found between the distributions for females and males without AMI, whereas no difference was found between females and males with AMI. The distributions of patients with and without AMI overlapped markedly giving a high number of misclassifications. The minimum fraction of misclassifications among all patients admitted occurred at a DL of 325 micrograms/l and was 0.16. When S-myoglobin is used for the purpose of early diagnosis of AMI the DL should be chosen so that the fraction of false negative patients is small. Consequently the fraction of false positive patients will be relatively high. At a DL of, for example, 175 micrograms/l, the false negative fraction was 0.06 of all patients with AMI (sensitivity 0.94), and the fraction of false positive patients was 0.35 (specificity 0.65).(ABSTRACT TRUNCATED AT 250 WORDS)

False Positive Reactions↗
PubMed1985

The posterior cruciate ligament and rotatory knee instability. An experimental study.

The importance of the posterior cruciate ligament in relation to valgus-varus and axial rotatory stability in the knee joint was investigated. Mobility patterns were drawn from 20 osteoligamentous preparations after successive transection of the posterior cruciate ligament (PCL), the medial and lateral collateral ligaments, and the posterior joint capsule. The knee joint remained grossly stable after isolated transection of the PCL, and further cutting of either one of the collateral ligaments or of the posterior capsule yielded no greater instability than one should expect from isolated cutting of each of these structures. The posterior cruciate ligament was the stabilizing factor in flexion and external rotation after injury to the lateral collateral ligament and the posterolateral capsule, and it restricted internal rotation after cutting of the medial cruciate ligament and the posteromedial capsule. Valgus instability was markedly increased during the whole range of movement when PCL was included in injury to the medial compartment ligaments, and when included in a lateral compartment injury a further varus instability was found, though only in the flexed or semiflexed knee. No hyperextension could be demonstrated after these injuries.

Biomechanical Phenomena↗
PubMed1985

Stability of the ankle joint. Analysis of the function and traumatology of the ankle ligaments.

The purpose of this study was to elucidate the function of the ligamentous structures of the ankle joint, the traumas in which they may rupture, and the types of instability caused by such ruptures. Most previous experimental investigations on the function of ankle ligaments have been performed on osteoligamentous preparations either by forcing a movement in the joint into more or less well-defined directions and observing the resulting injuries or else by cutting ligaments in various combinations and describing the resulting instability. As a rule, this has been done without inducing the increased mobility by a defined torque and without being able to demonstrate the instability continuously in all degrees of dorsi- or plantar flexion. On the basis of these previous studies, the anterior talofibular ligament appears to limit internal rotation, while its role in adduction of the talus has not been clarified. The calcaneofibular ligament per se does not seem to be a factor in adduction. True, there is not complete agreement in this respect, and a few authors have been able to rupture this ligament in isolation by forced adduction. The posterior talofibular ligament seems to restrict dorsiflexion, and perhaps it plays a role, in conjunction with the calcaneofibular ligament, in adduction when the ankle joint is in dorsiflexion. The anterior tibiofibular ligament, and the distal tibiofibular structures on the whole, are assumed to limit external rotation, but it has not been clarified whether they influence adduction and abduction in the ankle joint. Little has been reported about the individual structures which make up the deltoid ligament, as most authors have not distinguished them from each other. However, in combination with the anterior talofibular ligament, the tibiotalar ligament seems to limit the translatory forward gliding of the talus - the so-called anterior drawer sign - and together they presumably inhibit plantar flexion. It has not been possible to find any description of the function of the intermediate tibiotalar ligament, while the posterior tibiotalar ligament has been reported by one author to inhibit internal rotation. Judging by the literature, the function of the tibiocalcaneal ligament seems comprehensive, since it is reported to limit external rotation, dorsiflexion, as well as plantar flexion. The present investigations were divided into three phases: Phase 1 was concerned with elucidating the correlation of injuries to the lateral collateral ligaments to internal rotatory instability, talar tilt, and the anterior drawer sign.(ABSTRACT TRUNCATED AT 400 WORDS)

Ankle Joint↗
PubMed1985

Trabecular bone strength profiles at the ankle joint.

The mechanical strength of trabecular bone at the ankle joint was studied in ten amputation specimens by means of multiple penetration tests. Tibial bone was considerably weaker than talar bone (40% on average). An area of peak bone strength located posteriorly and medially to the center of the joint was found in most joints. The variation of bone strength with the depth from the surface was studied by comparing five successive 2-mm levels. At the tibia there was a statistically significant decrease of bone strength from the first to the second level. At the talus, bone strength increased at first, then decreased significantly. The absolute values of strength recorded suggest that in a substantial number of the joints studied, the resection surfaces--especially at the tibial side--might be too weak to support the loads imposed by current prosthetic designs.

Ankle Joint↗
PubMed1984

Rotatory instability of cadaver knees after transection of collateral ligaments and capsule.

The importance of the medial and lateral compartment ligaments of the knee in relation to valgus-varus and axial rotation instability was investigated. Mobility patterns were drawn from 20 osteoligamentous knee preparations after successive transections of the structures. Cutting of the medial collateral ligament resulted only in slight valgus instability and modest anteromedial instability. When the medial posterior joint capsule was also cut, both types of instability increased, and moreover, the knee became posteromedially unstable. Cutting of the lateral collateral ligament produced only a little varus instability and anterolateral rotatory instability, but varus instability increased considerably when the posterior lateral capsule was also transected. In this latter situation, even marked posterolateral rotatory instability was found. Isolated transection of the medial or lateral collateral ligament did not cause any major valgus or varus instability, but when the posterior capsule was also transected, a considerable degree rotatory instability could be found even though the cruciate ligaments were intact.

Biomechanical Phenomena↗
PubMed1984

The anterior cruciate ligament of the knee: an experimental study of its importance in rotatory knee instability.

The importance of the anterior cruciate ligament (ACL) in relation to valgus-varus and axial rotation stability in the knee joint was investigated. Mobility patterns were drawn from ten osteoligamentous preparations after successive transection of the two parts of the ACL and the medial (MCL) and lateral collateral ligaments (LCL). The knee joint remained grossly stable after partial injury of the ACL, while sectioning of the entire ACL caused an increase in internal rotation in the extended-semiflexed position. Combined lesions to the ACL and the MCL caused considerable valgus instability increasing with flexion, the joints remaining stable in extension. Moreover, marked anteromedial instability occurred, while only slight posteromedial instability was found. Combined lesions to the ACL and the LCL caused varus instability, worst in the semiflexed position, and a consistent pivot shift in applying a valgus torque in flexion was noted. Moreover, moderate posterolateral instability was found, at its maximum in the semiflexed position. External rotatory stability is secured primarily by the MCL, secondarily by the posterior medial capsule, and finally by the ACL. The existence of lateral pivot shift is proof of damage to the ACL.

Humans↗
PubMed1984

Instability of cadaver knees after transection of capsule and ligaments.

The importance of the medial collateral ligament and the anterior cruciate ligament of the knee in relation to valgus and varus instability was investigated. Mobility patterns were drawn from ten osteoligamentous knee preparations after successive transections of the structures. Cutting the entire collateral medial ligament caused only slight valgus instability, even when the knee was flexed. Further transection of the anterior cruciate ligament increased the instability considerably, but the knee remained stable in extension. The valgus instability after the transections was maximal at about 60 degrees of flexion.

Biomechanical Phenomena↗
PubMed1983

An analysis of the function of the posterior talofibular ligament.

The function of the posterior talofibular ligament was studied using an apparatus which subjected the ankle joint to a measured torque and allowed the simultaneous recording of rotatory movements in two planes. Thirty osteoligamentous preparations of ankle joints were examined, half in the sagittal and horizontal planes and the remainder in the sagittal and frontal planes. Successive section of the lateral collateral ligaments was performed, including, in particular, selective division of the short and long fibres of the posterior talofibular ligament. The function of this ligament was investigated in combination with the other two collateral lateral ligaments, with the calcaneofibular ligament alone, and finally as the only remaining intact ligament. The posterior talofibular ligament plays only a supplementary role in ankle stability when the lateral ligament complex is intact. After rupture of the anterior talofibular and the calcaneofibular ligaments, however, the short fibres of the posterior ligament restrict internal and external rotation, talar tilt, and dorsiflexion, while its long fibres inhibit only external rotation, talar tilt, and dorsiflexion. As the posterior talofibular ligament has no independent stabilizing function in the intact ankle joint, an isolated rupture of this ligament is unlikely.

Ankle Injuries↗
PubMed1983

Deltoid ligament. Functional analysis of the medial collateral ligamentous apparatus of the ankle joint.

On 34 osteoligamentous ankle preparations the function of the various components of the deltoid ligament has been elucidated by tracing mobility patterns after successive transection of the components in varying sequence. The anterior and posterior talofibular ligaments were included in the study to investigate the interaction between these structures and the deltoid ligament. The tibiocalcaneal and the intermediate tibiotalar ligaments control abduction of the talus. The anterior tibiotalar and talofibular ligaments control plantar flexion, while dorsiflexion is inhibited by the posterior tibiotalar and talofibular ligaments, and partly by the anterior talofibular ligament as well. In combination, the anterior and intermediate tibiotalar ligaments control external rotation, while the intermediate and posterior tibiotalar ligaments control both external and, together with the anterior talofibular ligament, internal rotation of the talus. Isolated, neither the anterior nor the posterior tibiotalar ligament appears to play any major role in ankle stability.

Ankle Joint↗