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Biomedical subjects

O Nishimura

Publications and source records attributed to O Nishimura.

At least 91 records · Page 5Linked to original sources
PubMed1986

Cloning of rat brain protein kinase C complementary DNA.

Four peptides derived from rat brain protein kinase C were partially sequenced. Using synthetic oligonucleotides deduced from the amino acid sequences as probes, a clone of complementary DNA (cDNA) was isolated from a cDNA library prepared from the same tissue. The nucleotide sequence of this cDNA clone revealed the primary structure of the carboxyl-terminal region as having 224 amino acids, with significant sequence homology with cyclic AMP-dependent and cyclic GMP-dependent protein kinases.

Amino Acid Sequence↗
PubMed1986

Separation of recombinant human interleukin-2 and methionyl interleukin-2 produced in Escherichia coli.

Escherichia coli harboring the gene coding for human interleukin-2 (IL-2) produced methionyl IL-2 (Met-IL-2) having an additional methionine residue at the amino terminus as well as IL-2 starting with the amino terminal alanine. IL-2 and Met-IL-2 were copurified from a cell-free extract. It was difficult to separate these two molecular species from each other because of the similarities of their physico-chemical characteristics. We found that the isoelectric points of IL-2 and Met-IL-2 were slightly but significantly different and succeeded in separating these two molecular species by utilizing the difference of their isoelectric points. The isoelectric points of IL-2 and Met-IL-2 thus obtained were determined to be 7.7 and 7.5, respectively. The in vitro specific activities of these two species were the same and similar to that of natural human IL-2 derived from peripheral blood lymphocytes.

Amino Acid Sequence↗
PubMed1986

Bone disorder following partial and total gastrectomy with reference to bone mineral content.

To study post-gastrectomy metabolic bone disorders, we measured the radial bone mineral content (BMC), serum levels of calcium, inorganic phosphorus, alkaline phosphatase, and 25-hydroxyvitamin D(25-OH-D) in 59 patients with partial- and 39 patients with total gastrectomy. Total gastrectomy patients manifested a higher incidence of decreased BMC levels than did partial gastrectomy patients (56 per cent vs. 25 per cent). Patients subjected to the Billroth II procedure, especially females, manifested abnormally low BMC values. The decline in BMC was age-related; it was pronounced in females. At 10 years postoperatively, many of the partial gastrectomy patients manifested markedly low BMC levels; in totally gastrectomized patients this finding was made at less than 5 years postoperatively. Approximately 30 per cent of our patients showed abnormalities in serum minerals, alkaline phosphatase or 25-OH-D.

Alkaline Phosphatase↗
PubMed1986

Gastric functions in patients with the intrathoracic stomach after esophageal surgery.

Functions of the stomach placed in the posterior mediastinum after esophagectomy were studied in 20 esophageal carcinoma patients. Seven were long-term survivors who lived more than 5 years after operation, and five of them showed normal fasting serum gastrin levels and good or fair gastric acid secretion. Of 13 patients who had their operations within 3 years before the study, 11 showed high fasting serum gastrin levels and poor gastric acid secretion. The hepatobiliary and alimentary scintigrams with double isotopes demonstrated a time lag between the excretion of the food from the stomach and the excretion of bile into the bowels, regardless of the postoperative periods. Absorption of vitamin B12 was normal in patients who lived more than 2 years after operation. The intraluminal pressure and pH studies in long-term survivors showed that our operative technique, the posterior invagination esophagogastrostomy, was effective in preventing a gastroesophageal reflux in the anastomosis.

Adult↗
PubMed1985

Amino acid sequence of heat-stable enterotoxin produced by Vibrio cholerae non-01.

The amino acid sequence of heat-stable enterotoxin, produced by Vibrio cholerae non-01 and isolated from its culture supernatant, was determined by both Edman degradation of native and reductively carboxymethylated enterotoxin and also a combination of fast atom bombardment mass spectrometry and carboxypeptidase Y digestion of native enterotoxin to be as follows: Ile-Asp-Cys-Cys-Glu-Ile-Cys-Cys-Asn-Pro-Ala-Cys-Phe-Gly-Cys-Leu-Asn. This sequence is very similar, but not identical, to those of heat-stable enterotoxins produced by enterotoxigenic Escherichia coli and Yersinia enterocolitica.

Amino Acid Sequence↗
PubMed1985

[Relationship between cholecystectomy and the subsequent development of colorectal carcinoma].

In order to clarify the presence or absence of a relationship between cholecystectomy and the subsequent development of colorectal carcinoma, we compared the incidence of previous cholecystectomy in 448 patients with colorectal cancer and in 1627 with gastric cancer, who had been treated in our clinic during the 21 year period through 1963 to 1983. There was no statistically significant difference in the incidence of previous cholecystectomy between patients with colorectal and gastric cancer. We then performed an historical cohort study covering 461 patients undergoing cholecystectomy for gallstone during the 32 year period from 1949 to 1981 for the subsequent occurrence of carcinoma of the colorectum and stomach. The observed incidence was one in colon cancer and six in gastric cancer, showing a similar proportion of the development of both carcinomas in our district. The present study showed no evidence of cholecystectomy being an increased risk for the development of colorectal carcinoma.

Adolescent↗
PubMed1985

[Serum gastrin and secretin and their trophic action after proximal and total gastrectomy].

Serum levels of gastrin and secretin were measured in experimental animals and in man following gastric surgery. Serum gastrin was higher in the fasting, and increased further after test meal in proximally gastrectomized subjects as compared in those receiving total gastrectomy and in normal controls. Serum secretin level, however, was unchanged before and after test meal irrespective to two surgical procedures. Dogs undergoing proximal gastrectomy showed increased output of bicarbonate and amylase as an exocrine pancreatic secretion after instillation of 0.1N HCl into the duodenum. Increased DNA synthesis was observed in the small intestine and pancreas in the proximally gastrectomized dogs. Therefore, higher level of serum gastrin may possess trophic activity in animals undergoing proximal gastrectomy. The findings obtained in this study suggest that we should select, if possible, the proximal gastrectomy leaving the antral region from the view point of trophic action of gastrointestinal hormones.

Animals↗
PubMed1984

Synthesis and fast-atom-bombardment-mass spectrometry of N-acetylmuramoyl-L-alanyl-D-isoglutamine (MDP).

N-Acetylmuramoyl-L-alanyl-D-isoglutamine (MDP) was synthesized by a series of condensations of appropriate reagents, followed by hydrogenolysis. Each intermediate step resulted in a stable, crystalline product. D-Isoglutamine 4-benzyl ester was condensed with N-(tert-butoxycarbonyl)-L-alanine N-hydroxysuccinimide ester, to give N-(tert-butoxycarbonyl)-L-alanyl-D-isoglutamine benzyl ester. Condensation of L-alanyl-D-isoglutamine benzyl ester with N-acetyl-1-O-benzyl-4,6-O-benzylidenemuramic acid, followed by hydrogenolysis, gave MDP. The synthetic scheme was shown to be capable of producing gram quantities of highly pure MDP, as well as a few of its analogs. The synthetic MDP was characterized by analytical and biological methods, and it was found that the use of fast-atom-bombardment-mass spectrometry may greatly simplify the characterization process.

Acetylmuramyl-Alanyl-Isoglutamine↗
PubMed1984

Efficacy of endoprosthesis for internal biliary drainage in patients with nonresectable carcinoma of the proximal bile duct.

Surgical endoprosthesis as an internal biliary drainage was performed as a palliative treatment for 12 patients with a nonresectable carcinoma of the proximal bile ducts. In most of these patients jaundice was relieved, they were able to return to their homes, and their survival was substantially prolonged. The efficacy of this procedure was more favorable, compared to the results in patients in whom external drainage or cholangiojejunostomy was performed.

Aged↗
PubMed1984

The activity of synthetic analogs of serum thymic factor (FTS) to convert mouse pre-T cells into Thy-1 positive cells.

Serum thymic factor (FTS) and its 33 analogs were compared with regard to the ability to induce Thy-1 antigen on mouse pre-T cells. The pre-T cells were prepared from spleen cells of athymic nu/nu mice by passing them through a nylon wool column, and the induction of Thy-1 antigen was analysed using a fluorescence-activated cell sorter. Thy-1 negative cells were converted into Thy-1 positive cells by FTS and some of its analogs. Deletion of pGlu at the amino terminus of FTS did not alter the activity. Des ( pGlu1 , Ala2)-FTS and des ( pGlu1 , Ala2, Lys3 )-FTS were inactive and deletion of the carboxyl terminal residue, Asn, also resulted in the loss of activity. Substitution or modification of pGlu1 and Asn9 with an amino acid, an acyl group or an amine caused a marked reduction of the activity. The residue 3, Lys, could be substituted without any loss of the activity by Arg, but not by Leu. These results show that the octapeptide moiety, Ala-Lys-Ser-Gln-Gly-Gly-Ser-Asn, is the minimum essential part of the FTS molecule for the expression of activity and they suggest that the positive charge at the residue 3 is also important for its activity.

Animals↗
PubMed1984

Aminergic innervation of cerebral veins. Histochemical comparison with extracranial veins.

The density and distribution pattern of aminergic nerve fibers in intracranial and extracranial veins were compared by means of catecholamine histofluorescence studies. Extracranial veins (internal jugular, inferior caval, portal, renal, internal iliac, and femoral veins) showed quite a uniform distribution pattern. Large veins (jugular, caval, renal, and iliac veins) revealed a spiral pattern, and small veins presented as a meshwork (femoral vein) or in a longitudinal pattern (a branch of the femoral vein). In contrast, intracranial veins displayed more complex patterns: the dural sinus showed a longitudinal pattern and the internal cerebral vein and the superficial vein of Labbé revealed a meshwork of nerve fibers. The aminergic fibers were denser in cerebral veins (dural sinus and internal cerebral vein) than in extracranial veins. The complex distribution and the density of aminergic fibers in cerebral veins suggest that the aminergic innervation of the circulatory system may be more important in the brain than in the extracranial organs.

Amines↗
PubMed1984

[A clinical study on cefotiam with special reference to excretion in bile and concentration in gallbladder tissue].

Following 1 hour intravenous drip infusion of 1.0 g of cefotiam (CTM), 38 patients with cholelithiasis were operated at scheduled intervals. The CTM concentrations in serum, common duct bile, gallbladder bile and gallbladder tissue were measured. The mean level of CTM concentration in serum showed a peak of 53.1 micrograms/ml at 1 hour, and decreased in the level of 1.0 microgram/ml at 5 hours after administration. CTM concentrations in common duct bile revealed a mean level of 162 micrograms/ml at 1 hour and similarly high concentrations from 1 to 5 times of the peak level in serum were maintained for 3.5 hours. In patients with impaired liver function, the levels of CTM concentration were lower than those without them. CTM concentrations in gallbladder bile showed a high value at 2-3.5 hours after administration . In many cases the levels were relatively lower than those in common duct bile, particularly ion patients with cystic duct obstruction and/or with marked chronic cholecystitis. As for gallbladder tissue levels, CTM concentrations showed low levels less than 50 micrograms/g in patients with pathological changes of gallbladder, as well as in gallbladder bile. While, favorable concentrations of 50-100 micrograms/g were obtained in patients without them. There was a positive correlation between CTM concentrations in common duct bile and gallbladder bile, and also between the concentrations in gallbladder bile and those in gallbladder tissue in patients without cystic duct obstruction or marked chronic cholecystitis. Then, in the situation, CTM transfer into gallbladder bile or gallbladder tissue might be mainly dependent on biliary excretion of CTM.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1984

[Carcinoma of the upper portion of the stomach; proximal partial gastrectomy or total gastrectomy].

Significance of proximal partial gastrectomy for carcinoma of the upper portion of the stomach is reported. The 5-year postoperative survival rate was 58% in patients receiving proximal partial gastrectomy while it was 61% in those undergoing total gastrectomy. With respect to stage III cancer cases, there was no difference in the 5-year postoperative survival between the 2 groups. Therefore, we suppose that proximal partial gastrectomy is a treatment of choice for patients with carcinoma of the upper portion of the stomach. Late postoperative laboratory examination showed less incidence of hyperchromic anemia in patients receiving proximal partial gastrectomy, and lowered serum vitamin B12 level was noted in patients undergoing total gastrectomy. Hypergastrinemia was found in patients receiving proximal partial gastrectomy, although its physiologic significance remains to be elucidated.

Cardia↗