Search PubMed⌕ Search

Biomedical subjects

O Nilsson

Publications and source records attributed to O Nilsson.

At least 325 records · Page 18Linked to original sources
PubMed1975

Leukocyte migration in agarose, a study on multiple sclerosis.

The effect of low concentrations of bovine encephalitogenic protein on the migration of human peripheral leukocytes in agarose was studied. A concentration of 0.3 mug/ml of the protein stimulated the migration of cells from many donors, including some healthy subjects. An indirect technique suggested that the migration enhancement is due to the production of soluble factor, possibly corresponding to the leukocyte migration enhancement factor described by others. The frequency of subjects whose cells could be stimulated and the recorded degree of stimulation tended to be higher in a group of patients with multiple sclerosis than in a group of healthy subjects. When the effect of some of the main peptide fragments of the protein was studied on cells that were stimulated by the intact protein, one or more of these peptides sometimes induced the opposite effect: a migration inhibition. There is, apparently, a complex balance between enhancing and inhibiting factors acting on leukocyte migration in vitro; and the character of the antigen seems to be one important factor.

Amyotrophic Lateral Sclerosis↗
PubMed1975

Effect of encephalitogenic protein on the migration in agarose of leucocytes from patients with multiple sclerosis. Correlations with clinical parameters, localization of antigenic determinants, and influence of corticotrophin treatment.

We have extended the results from our previous investigation of the reactivity of leucocytes from patients with multiple sclerosis and some other neurological diseases to bovine encephalitogenic protein, using the migration in agarose technique. 1. Reactivity (i.e. behaviour in the test deviating from that expected with cells from healthy subjects) is manifested either as an enhancement or an inhibition of leucocyte migration. 2. The incidence of subjects with cells showing reactivity to encephalitogenic protein is higher in the group of patients with multiple sclerosis than in the group of patients with other neurological diseases. 3. Reactivity to encephalitogenic protein is correlated to disease course score; patients with a chronic progression of the disease show the strongest reactivity in the test. 4. The tryptophan-containing region of the encephalitogenic protein appears to be a major determinant of significance for this test. 5. Patients tested during corticotrophin treatment tend to show a higher degree of reactivity in the test than expected-the increased reactivity seems to be primarily directed to the tryptophan-containing region.

Adrenocorticotropic Hormone↗
PubMed1975

Mixed leukocyte reaction and hl-a specificity at multiple sclerosis.

Compared with the mixed leukocyte reaction (MLR) between cells from healthy individuals, the MLR between cells from two patients with multiple sclerosis (MS) is, on average, reduced in strength. There is a tendency for the most marked reduction of MLR to occur when both or neither of the members in a tested pair carry HL-A7, and when cells from patients in a relatively early stage of the disease (short duration, relapses but no permanent disablement) are studied; especially when cells from these patients are used as reactor clels in the MLR. Repeated studies of the same cell combinations with varying intervals showed that when both members of the pair carry HL-A7,--and therefore have a large chance also to carry LD-7a--the MLR strength is relatively constant. When only one or neither of the members carry HL-A7, the strength of MLR varies considerably. Probably two different mechanisms exist in MLR impairment: similarity of LD genes, and "immunosuppressive" factors which can vary during the course of the disease.

Epitopes↗
PubMed1975

Embryo-endometrial relationship in the mouse during activation of the blastocyst by oestradiol.

Blastocysts that have been activated for implantation by an injection of oestrogen become separated from the uterine surface by a layer of secretion. By 8 hr after the injection, the trophoblast cytoplasm contains several glycogen granules, groups of ribosomes, and mitochondria of a condensed type. These ultrastructural changes are signs of the increased metabolic activity exhibited by the trophoblast at activation. It is suggested that the uterine epithelium keeps the trophoblast inactive by delivering only a minimal nutritive medium and that the epithelium activates the trophoblast by making more nutrients, probably some carbohydrate, available as a consequence of an oestrogen-induced increase in the secretory activity.

Blastocyst↗
PubMed1975

The hormonal control and morphology of blastocyst invasion in the mouse uterus.

When mature and immature uteri from ovariectomized mice were cultured in chemically defined media, blastocyst invasion occurred in the presence of progesterone but not in media containing only oestradiol. The invaded stromal tissue did not decidualize unless the uteri were taken from mice pretreated with progesterone and oestradiol. Fibrillar material was, however, concentrated in the stromal tissue adjacent to the invasive trophoblast. Neither progesterone nor oestradiol, had any ultrastructural effect on the endometrium of cultured uteri from ovariectomized mice, other than closure of uterine lumina in the presence of progesterone. Embryogenesis became abnormal, probably owing to the failure of formation of a suitable implantation chamber.

Animals↗