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Biomedical subjects

O Nielsen

Publications and source records attributed to O Nielsen.

92 records · Page 6Linked to original sources

Device and methods for the measurement of energy transfer in experiments involving thermal and electrical injuries of skin.

This work is part of a larger investigation aiming at tracing differences between injuries caused by heat and electricity. Electrodes and heaters similar to the electrodes of an electric cattle baton are used to dissipate approximately 40 joules in 40 seconds in pig skin. Voltages in the range 40-60 volts (AC or DC) are applied via stainless steel electrodes and temperatures in the range 50-100 degrees C are applied via heaters made of copper wire. Heat transfer is controlled by electronically regulated heaters utilizing the temperature dependence of the heater wire itself as a thermometer. Transfer of electricity takes place from either commercial DC supply, from transformed voltage from the mains or from a sine voltage generator connected to a power amplifier. The energy transfer is in all cases measured by electronic integration of the dissipated power in time. The power supplied to th electrodes or the heaters is found by electronic multiplication of the applied voltage and current. Typical power vs. time curves are presented.

Animals↗

Correlation between molecular genetic analyses and immunohistochemical evaluation of the epidermal growth factor receptor and p185HER2.

Several methods have been developed for the measurement of gene amplification and expression. This study compared different molecular genetic analyses (Southern blot analysis (SBA) and polymerase chain reaction (PCR)) with immunohistochemical (IHC) evaluation of the corresponding protein content. PCR may be used as a semi-quantitative analysis of gene amplification and allows DNA extraction from paraffin-embedded blocks. SBA is more accurate than PCR to measure the exact degree of amplification, but only DNA extracted from frozen or fresh tissue can be used. We examined 23 breast tumors and 16 lung tumors, where the genes HER-1 coding for the epidermal growth factor receptor (EGFR) and HER-2 coding for p185HER-2 were analysed. Furthermore, PCR performed on DNA from frozen tissue was compared to PCR on DNA extracted from paraffin-embedded blocks. The results showed correlation between the different analyses, especially when the gene copy number was highly amplified. Some breast tumors showed moderately increased gene copy number of HER-1 by SBA, but no increased protein content by IHC evaluation. This probably reflects that minor degrees of genetic aberrations are not sufficient to cause major biological disturbances, because regulatory cellular pathways are still operating.

Blotting, Southern↗