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Biomedical subjects

O Magnus

Publications and source records attributed to O Magnus.

At least 19 recordsLinked to original sources
PubMed2000

Inter-observer variation in the results of the clinical andrological examination including estimation of testicular size.

Inter-observer variation in andrological examination by 10 clinical investigators from five Nordic and Baltic countries was investigated. In addition, information on intra-observer variation was obtained for six of the 10 investigators. Testicular size was measured using Prader's orchidometer and one of the investigators also performed an ultrasound estimate of testicular size. A highly significant difference (p < 0.001) between observers was found with an inter-observer error of 16% in estimating testicular size in 23 young men. The difference in the estimate tended to increase with increasing testicular size. There was no significant intra-observer difference in two measurements performed on consecutive days. Only differences in median testis size, which were greater than 31% between measurements by two investigators, were found to be significant at the 5% level. The ultrasound estimate of testicular size was significantly lower than the orchidometer estimate, with a mean difference of 3.6 mL for the left testis and 4.3 mL for the right testis. Tanner staging of genitalia and diagnosis of a varicocele was subject to great inter-observer variation, and for the diagnosis of varicocele only one-third of the investigators was able to reproduce their results on a second examination. In conclusion, it was found that the clinical andrological examination of young men is subject to great inter-observer variation. This should be kept in mind when results from different studies are compared as well as in daily clinical practice.

Adult↗
PubMed1998

Homozygous mutation (A228T) in the 5alpha-reductase type 2 gene in a boy with 5alpha-reductase deficiency: genotype-phenotype correlations.

The molecular basis of a patient with 5alpha-reductase deficiency was investigated in this study. This disease is a rare form of male pseudohermaphroditism with virilization during puberty. The child was raised as a girl, but had a male gender identity early in life. The diagnosis was set at the age of 13 years when the virilization process began. Hypospadias repair was performed and he changed to a male gender. DNA sequence analysis disclosed a homozygous mutation in exon 4 of the 5alpha-reductase type 2 gene, alanine 228 for threonine. The heterozygous parents are first cousins of Pakistani origin.

Adolescent↗
PubMed1998

[Intracytoplasmic sperm injection].

The first results of intracytoplasmic sperm injection (ICSI) in Norway are presented. Acceptable fertilization rates were obtained for all indications; severe male infertility, as well as moderately reduced sperm quality where in vitro fertilization (IVF) and embryo transfer had previously failed. Furthermore, in cases where IVF had failed despite normal sperm quality, fertilization rates of 50-60% were achieved. The overall pregnancy rate was 24.9% per embryo transfer, and the live pregnancy rate per started cycle was 13.4%. As expected, the results improved with experience. The results were greatly influenced by the age of the female. In the age group 34 years or less, the total pregnancy rate per cycle was 32.4%, with a miscarriage rate of 24.2%. In the age group 35 years or more, (42% of the study group), the total pregnancy rate per embryo transfer was 15.2%, with a miscarriage rate of 75%. The corresponding implantation rates were 17.4% and 7.9% respectively. 23 children have been born so far, none with major malformations or chromosome abnormality symptoms.

Adult↗
PubMed1992

In vivo fertilization procedures in infertile women with patent fallopian tubes: a comparison of gamete intrafallopian transfer, combined intrauterine and intraperitoneal insemination, and controlled ovarian hyperstimulation alone.

This prospective study was undertaken to evaluate the relative efficacy of three in vivo methods of assisted fertilization in 150 infertile women with patent fallopian tubes: gamete intrafallopian transfer (GIFT), combined intrauterine and direct intraperitoneal insemination (IUI+DIPI), and controlled hyperstimulation (COHS) alone. The clinical pregnancy rate was highest in the IUI/DIPI and GIFT groups: IUI/DIPI, 29.3%; GIFT, 28.6%; and COHS, 8.9%. We believe that controlled ovarian hyperstimulation combined with IUI and DIPI is a good alternative to GIFT.

Adult↗
PubMed1991

The first attempt at IVF treatment. Results and requirements for a satisfactory success rate.

The purpose of this study was to report the success rate of the first IVF treatment in 161 patients who attended our department in 1988. The indications for IVF were tubal damage in 84 couples (52.1%), unexplained infertility and endometriosis (with patent tubes) in 61 couples (37.9%), and polycystic ovarian syndrome in 16 couples (10%). Clinical pregnancies were diagnosed in 40 patients, giving an overall clinical pregnancy rate of 35.4% per embryo transfer. In the group of patients with unexplained infertility or endometriosis one in three of the embryo transfer cycles will predictably result in a birth, one in four in the group of patients with tubal damage or polycystic ovarian syndrome. These results invite discussion of the possible reasons for the success of our IVF program.

Adult↗
PubMed1991

Effects of seminal plasma from normal and asthenozoospermic men on the progressive motility of washed human sperm.

Human seminal plasma stimulated the progressive motility of human sperm in a dose- and time-related manner. Serum exhibited a similar capacity for stimulation. Seminal plasma from different normozoospermic men showed marked variation in its capacity to stimulate sperm motility. The stimulatory effect was maintained after heat-treatment, indicating the action of a low molecular weight substance or metal ion. No differences could be observed in the capacity for stimulation between seminal plasma from normozoospermic (n = 23) and asthenozoospermic (n = 22) men, when tested at the same dilution and under identical conditions. It is concluded that in general, differences in seminal plasma composition cannot account for the reduced sperm motility in asthenozoospermic men. Furthermore, the stimulatory effects of seminal plasma may be a property shared by other biological fluids.

Hot Temperature↗
PubMed1990

Ionized calcium in human male and female reproductive fluids: relationships to sperm motility.

The levels of ionized calcium in seminal plasma were approximately 20% of the serum levels. In contrast, cervical mucus contained a level of ionized calcium similar to both serum and follicular fluid. Titration of seminal plasma and serum with increasing concentrations of calcium chloride indicated a 10-fold higher calcium-binding capacity for seminal plasma. In a random group of men under semen investigation, concentrations of ionized calcium and citrate in semen were inversely correlated (r = 0.732; P less than 0.001), an observation which was confirmed by studies of split ejaculates. These findings supported the contention that citrate is the major regulator of the levels of ionized calcium in seminal plasma and primarily responsible for maintaining the calcium gradient between the seminal plasma and cervical mucus. No significant relationship could be demonstrated between the levels of ionized calcium in the ejaculates and any of the motility characteristics of the spermatozoa in the same sample. Furthermore, the addition of increasing quantities of calcium chloride (0.16-20.00 mM) to washed spermatozoa had no major effects on their progressive motility. These data suggest that human spermatozoa are effective in maintaining an appropriate level of internal ionized calcium, necessary for normal motility, despite fluctuations in external calcium.

Ascitic Fluid↗
PubMed1990

Effects of manganese and other divalent cations on progressive motility of human sperm.

Manganese (Mn2+) stimulated the progressive motility of human washed sperm in a time- and dose-dependent manner. This confirmed previous data indicating a capacity for activating the adenylyl cyclase of sperm homogenates in vitro. A maintained response was best seen with doses 0.2-1.0 mM. After an initial stimulation, higher concentrations of up to 20 mM were associated with a decline in the response to control levels. Magnesium ions (Mg2+) stimulated motility in the same dose range but to less than 50% of the Mn2+ response. Other divalent cations such as zinc (Zn2+), strontium (Sr2+), and Calcium (Ca2+) also exerted stimulatory effects to varying degrees in descending order. Metal ion effects on sperm motility may be mediated through a common cation-binding site on the adenylyl cyclase.

Cations, Divalent↗
PubMed1989

Gonadotropin and ovarian steroid production in polycystic ovarian syndrome during suppression with a gonadotropin-releasing hormone agonist.

Six women with polycystic ovarian syndrome were treated with a gonadotropin-releasing hormone agonist prior to ovulation induction with gonadotropins. Buserelin nasal spray (600 micrograms/day) was given for 6 weeks. There was a gradual and significant decrease in the level of luteinizing hormone (LH) during the treatment period. No change was observed in the level of follicle-stimulating hormone. The estradiol, estrone, testosterone and androstenedione levels decreased significantly. No reduction was seen in the DHEAS level. After 4 weeks of treatment LH had reached postmenopausal levels, and testosterone and androstenedione were within the normal range. It is concluded that 4 weeks' treatment with 600 micrograms buserelin intranasally per day is sufficient to normalize the ovarian androgen production in polycystic ovarian syndrome prior to gonadotropin stimulation and to reduce the LH level avoiding premature luteinization or spontaneous LH surge.

Administration, Intranasal↗
PubMed1989

Gonadotropin therapy of female infertility.

Anovulatory infertility in 134 women was treated with gonadotropins for a total of 318 cycles. The patients were classified into WHO group I, hypothalamic-pituitary failure (72 patients), and WHO group II, hypothalamic-pituitary dysfunction (62 patients). All patients in this group had failed to achieve pregnancy with clomiphene citrate therapy in repeated cycles. The pregnancy rate in group I was 72.2% vs 17.7% in group II. The 'take home' baby rate was 57.1% in group I vs 13.1% in group II. The rate of miscarriages was 14.3% without any significant difference between the groups. Multiple pregnancies occurred only in group I patients (19.2%). The conception rate was highest in the first four cycles, whereas no patient became pregnant after the sixth treatment cycle. Ovarian hyperstimulation syndrome occurred most frequently in group II patients, however, overall only 2.2% of the patients needed hospitalization because of hyperstimulation. Gonadotropin therapy must be considered an efficient and successful treatment of infertility in patients with hypothalamic-pituitary failure, whereas the success rate is rather poor in patients with hypothalamic-pituitary dysfunction.

Chorionic Gonadotropin↗
PubMed1989

Gamete intrafallopian transfer (GIFT). The results of 93 consecutive treatments.

Eighty-two couples were treated with gamete intrafallopian transfer (GIFT) in 93 treatment cycles. Twenty-three clinical pregnancies resulted. Pregnancies were obtained in 40% of the cases where 5 or more ova were placed in the Fallopian tubes. In the cases where 4 or fewer ova were transferred, the pregnancy rate was 12%. Three miscarriages and one ectopic pregnancy occurred. Sixteen of the pregnancies were singleton, there were 5 twins, 1 triplet and 1 quadruplet. According to the present material, GIFT seems to represent a significant improvement in selected groups of patients.

Adult↗