Intraduodenally administered somatostatin-14 (a milk-borne peptide) has been shown to influence the pancreatic secretions in rats and dogs. To delineate the mechanism involved in the intraduodenal somatostatin-14, the fate of intraduodenal somatostatin-14 was investigated by administering [125I][Tyr11]somatostatin-14 ([125I][Tyr11]SS14) into the lumen of isolated duodenum in vivo of suckling and adult rats. At 2, 5, 10, and 30 min after administration, the radioactivity in the duodenal contents, duodenal wall, blood, liver, and kidney was measured, and the extracted radioactivity was analyzed for intact [125I][Tyr11]SS14 by reversed-phase high performance liquid chromatography. Radioactivity disappeared rapidly from the duodenal lumen with 50% of loss occurring at 2 min. No trace of intact [125I][Tyr11]SS14 was detected in any of the samples, except duodenal contents of suckling rats at 2 min, suggesting a rapid metabolism of SS14 in the duodenal lumen and a lack of duodenal absorption of intact SS14 in the rat.

Animals↗

PubMedDate not supplied

Diet, age and intestinal bile acids in pigs.

Total bile acid concentrations in gallbladder bile and duodenal juice of neonatal piglets receiving sow milk were compared to values from 10-wk-old pigs receiving either high-fat low-carbohydrate (HF/LC) or low-fat high-carbohydrate (LF/HC) diets. Ten-week-old pigs on either diet had higher bile acid concentrations in gallbladder bile than newborn pigs (108.6 +/- 2.5 and 109.3 +/- 1.4 vs. 50.0 +/- 10.0 mM, mean +/- SE for HF/LC, LF/HC, and newborn, respectively, p < 0.007; n = 8-10). Ten-week-old pigs in the LF/HC group had higher bile acid concentrations in duodenal juice than either the HF/LC or newborn animals (31.7 +/- 4.2 vs. 16.8 +/- 2.5 and 14.7 +/- 1.8 mM, respectively, p < 0.0001). These data demonstrate that like the human neonate, the newborn pig has decreased bile acid concentrations available for digestion compared with the adult and resembles the adult pig adapted to a high-fat diet.

Aging↗