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Biomedical subjects

O Kawamura

Publications and source records attributed to O Kawamura.

At least 55 records · Page 3Linked to original sources
PubMed1989

Studies of a glomerular permeability factor in patients with minimal-change nephrotic syndrome.

Peripheral blood mononuclear cells (PBMC) from patients with minimal-change nephrotic syndrome (MCNS) were tested for their ability to produce a factor which increases the urinary protein excretion levels of rats. It was shown that enhanced proteinuria can be produced in 8-hour urine specimens from rats by the injection of concentrated supernatants of cultured concanavalin-A-stimulated PBMC of patients with MCNS, but not from other nephrotics or normal subjects. The increase in urinary protein excretion was associated with a significant alteration of glomerular epithelial cells similar to that seen in MCNS. These results suggest that in MCNS, PBMC release a factor, which we termed a glomerular permeability factor (GPF), causing changes in glomerular permeability with resulting proteinuria.

Adolescent↗
PubMed1988

Improved preparation of T-2 toxin-protein conjugates.

T-2 toxin (T-2) was converted into T-2 hemisuccinate (T-2 HS), T-2 hemiglutarate (T-2 HG) and T-2 hemiphthalate (T-2 HP), and conjugated with bovine serum albumin (BSA), ovalbumin (OVA) and keyhole limpet haemocyanin (KLH) using carbodiimide under various conditions. The recovery of T-2 conjugated proteins and the amount of T-2 bound were largely dependent on the amount of coupling reagent, pH, reaction temperature, buffer and reaction time. In regard to T-2 derivatives binding with a protein, T-2 HG was the most efficient, followed by T-2 HS and then T-2 HP. With regard to T-2 to protein binding, KLH was the most efficient, followed by BSA and then OVA. To promote T-2-protein binding, phosphate-buffered saline was more effective than cacodylate buffer. Within a pH range of 5.5-7.0, weakly acidic conditions were more efficient than neutral conditions for promoting T-2-protein binding.

Animals↗
PubMed1988

Application of an enzyme-linked immunosorbent assay for screening of T-2 toxin-producing Fusarium spp.

Culture filtrates of Fusarium species were subjected without clean-up procedures to an indirect competitive enzyme-linked immunosorbent assay with anti-T-2 toxin monoclonal antibody. Fusarium sporotrichioides, F. poae, F. tricinctum, and F. culmorum strains were positive for T-2 toxin, with a minimum detection limit of 5 pg per assay (100 pg/ml of culture filtrate), and the assay data correlated well with the gas-liquid chromatographic data.

Antibodies, Monoclonal↗
PubMed1988

[A case of a probable "cancer family syndrome" presenting four synchronous malignancies of the colon].

A 72-year-old male was admitted because of right lower quadrant pain, Barium enema and total colonoscopy disclosed multiple colon cancers and sequentially, a subtotal colectomy was performed. The resected specimen demonstrated 3 advanced carcinomas and an adenomatous cancer with additional multiple polyps. Investigation of his family history revealed that his mother and his elder sister had died of uterine cancer, and that his elder brother, his nephew, and his niece had been operated on for colorectal cancer. We thus supposed a case of "Cancer Family Syndrome" presenting multiple neoplasms of the colon.

Adenocarcinoma↗
PubMed1987

Significance of renal hyaline arteriolosclerosis and tubulo-interstitial change in IgA glomerulonephropathy and focal glomerular sclerosis.

The relationship between glomerular sclerosis and hyaline arteriolosclerosis (HA) or tubulo-interstitial (T-I) change was investigated in 66 patients with IgA glomerulonephropathy (IgA-GN) and in 12 patients with idiopathic focal segmental glomerulosclerosis (FGS). More than a 10% incidence of global sclerosis was noted in 15 patients with IgA-GN and in 9 patients with FGS. There was no significant discrepancy of age between patients with IgA-GN and those with FGS (IgA-GN: 37.5 +/- 12.5; FGS: 35.7 +/- 10.3). In patients with IgA-GN, 7 out of 15 were HA-positive (46.7%), whereas in patients with FGS, 7 out of 9 were HA-positive (77.8%). However, a close correlation was observed between glomerular sclerosis and T-I change only in patients with IgA-GN (r = 0.82; p less than 0.001). The results suggested that in the case of IgA-GN, T-I change was related to the prognosis of glomerular sclerosis. In addition, it is assumed that a possible correlation exists between FGS and HA.

Adolescent↗
PubMed1986

Idiopathic right ventricular dilation. Special reference to "arrhythmogenic right ventricular dysplasia" and analogous lesions.

Two autopsy cases which showed marked depletion of the right ventricular musculature of the heart accompanied with marked infiltration of the adipose tissue were reported. The first cases was an 18-year-old female who died of right sided congestive heart failure after about 4-years clinical course. The autopsy disclosed marked dilation of the right atrium and ventricle. The entire free wall of the right ventricle was markedly thin. Microscopically, most of the myocardial fibers of the right ventricle were replaced by fat and fibrous tissue. The second case, a 15-year-old boy, whose identical twin was previously diagnosed as arrhythmogenic right ventricular dysplasia designated by Fontaine et al., died suddenly during exercise. He showed no cardiac symptoms but electrocardiogram was abnormal. Autopsy revealed majority of the myocardial fibers of the right ventricular free wall were replaced by fatty tissue. In both cases, fatty infiltration was mainly noticed at the epicardial side and some myocardial fibers remained in the fatty tissue showed hypertrophic and/or degenerative changes. Review of the literature on similar cases showing depletion of the right ventricular musculature including so-called adult's Uhl anomaly, ARVD and dilated right ventricular myocardiopathy was conducted and the relationship of the present cases with these lesions was discussed.

Adipose Tissue↗