Wall peptidoglycan in Aerococcus viridans strains 201 Evans and ATCC 11563 and in Gaffkya homari strain ATCC 10400.
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Biomedical subjects
Publications and source records attributed to O Kandler.
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The production of d-lactic acid, a major fermentation product of Scenedesmus obliquus strain D(3), is inhibited by light. This inhibition has been observed in the wild type as well as in Bishop's mutant 11. However, in mutant 8, which lacks photophosphorylation, light stimulated the formation of d-lactic acid.
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The amino acid composition and amino acid sequence of the murein (peptidoglycan) of 10 strains of planococci were studied. It is shown that the peptide subunit consists of muramyl-l-alanyl-gamma-d-glutamyl-l-lysyl-d -alanine. The cross-linking of two adjacent peptide subunits is mediated by d-glutamic acid which is bound to the epsilon-amino group of lysine by its gamma-carboxyl group and to the carboxyl group of d-alanine of an adjacent peptide subunit by its amino group. About 20 to 25% of the peptide subunits are not cross-linked. The murein structure of the different species and strains of Micrococcus, Staphylococcus, and Sarcina are compared. It is evident that the murein structure is a very good criterion for grouping the micrococci. In addition, some of these groups are fairly well defined by physiological properties as well as by their guanine + cytosine content of the deoxyribonucleic acid e.g., Micrococcus, Staphylococcus, Planococcus, Sarcina ureae. Other groups, represented by a single or a few strains only, such as M. varians NTCC 7281, M. radiodurans, M. freudenreichii ATCC 407, and M. luteus ATCC 398, need further investigation.
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Salicylaldoxime (2 x 10(-3)m and less) inhibits cyclic photophosphorylation in intact Chlorella cells severely whereas photosynthetic O(2)-evolution and (14)CO(2)-fixation is hardly affected. Cyclic photophosphorylation in vivo was measured by following anaerobic light dependent glucose uptake. A similar difference in susceptibility has been observed with carbonylcyanide-p-trifluoromethoxyphenylhydrazone. Various controls exclude the possibility that the difference in inhibition was caused by differing experimental conditions or, in the case of glucose assimilation, by an inhibition of a reaction other than photophosphorylation.There also exists a great difference in light saturation of cyclic photophosphorylation and photosynthesis. Evidence is reported that at light saturation of glucose uptake light driven cyclic phosphorylation is indeed the limiting reaction.The results are considered as evidence that cyclic photophosphorylation does not contribute ATP stoichiometrically to photosynthetic CO(2)-fixation.
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