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Biomedical subjects

O J Miller

Publications and source records attributed to O J Miller.

At least 163 records · Page 9Linked to original sources
PubMed1971

Mitotic separation of two human X-linked genes in man--mouse somatic cell hybrids.

SIX INTERSPECIFIC SOMATIC HYBRID CELL LINES WERE DERIVED FROM A MOUSE LINE DEFICIENT IN HYPOXANTHINE: guanine phosphoribosyltransferase (HGPRT) and human diploid cells with normal enzyme activity. Human HGPRT was present in all six hybrids and the clones derived from them. However, in two of the six, and in some clones from another two, human glucose-6-phosphate dehydrogenase (G6PD) was absent. Since the structural loci for both these enzymes are X-linked in man, these findings suggest that these two loci have separated quite frequently through chromosome breakage and that they must be rather far apart on the X chromosome.

Animals↗
PubMed1971

Assignment of four linkage groups to chromosomes in Mus musculus and a cytogenetic method for locating their centromeric ends.

The mitotic chromosomes in primary cultured cells from mouse embryos of the translocation stocks, T(5;18)26H, T(5;11)7Ca, and T(14;17)264Ca, have been identified by their distinctive fluorescent-banding patterns after staining with quinacrine mustard. In this way, linkage group (LG) V has been assigned to chromosome 2, LGXVIII to chromosome 9, and LGXIV and LGXVII to either of chromosomes 3 or 13. The assignment of LGXI to chromosome 6 has been confirmed. The centromeres of chromosomes 1 (LGXIII), 2 (LGV), 9 (LGXVIII), and 16 (LGIX) have been located at the ends nearest the genes fuzzy (fz), Danforth's short tail (Sd), nervous (nr), and T of their respective linkage groups by a cytogenetic method. The centromere of the X (LGXX) has been tentatively assigned to the end nearest to the scurfy (sf) locus.

Animals↗
PubMed1971

Identification of the mouse karyotype by quinacrine fluorescence, and tentative assignment of seven linkage groups.

A karyotype of the mitotic chromosomes of the house mouse has been prepared based upon quinacrine fluorescence patterns. All 19 pairs of autosomes and the X and Y chromosomes have been identified. Examination of the chromosomes of the following translocation stocks, T(11;?)1Ald, T(3;?)6Ca, T(2;9)138Ca, T(2;12)163H, and T(9;13)190Ca, have led to the tentative assignments of autosomal linkage groups (LG) to chromosomes as follows: LGII to chromosome number 10 (or 13), LGIII to 12 or 15, LGIX to 16, LGXI to 6, LGXII to 19 and LGXIII to 1. By definition, LGXX is on the X chromosome.

Animals↗