Biomedical subjects

O Ichiyanagi

Publications and source records attributed to O Ichiyanagi.

27 records · Page 2Linked to original sources

PubMed1996

Direct effect of amezinium on rabbit urethra: effect of estrogen and progesterone treatment.

The effect of amezinium, a new anti-hypotensive agent, and hormonal treatment on the female rabbit urethra was investigated. Cumulative dose responses were obtained for amezinium and norepinephrine on strips of muscle from the urethras of ovariectomized female rabbits by means of the tissue-bath system. Amezinium enhanced the response to electrical field stimulation and showed a direct contractile response on the urethra. These responses were only about 20% of the maximum norepinephrine response. The contractile response to amezinium was completely blocked by prazosin. When rabbits were pretreated with estrogen, with or without progesterone, for 4 weeks, the response to amezinium increased to 40% of the maximum norepinephrine response. Although amezinium enhances muscle contractile responses to electrical stimulation, this effect is strong when amezinium is used alone; concurrent estrogen treatment improves the effects of amezinium.

Adrenergic alpha-Agonists↗

PubMed1996

Role of symptom scoring and uroflowmetry in patients with diabetic cystopathy.

Forty-two patients with diabetes mellitus were evaluated by uroflowmetry and the AUA Symptom Index. After urodynamic and physiological investigations 12 patients were diagnosed as having diabetic cystopathy, while 22 patients as being urodynamically normal. In patients with diabetic cystopathy, most of the parameters in uroflowmetry were significantly different from those of normal subjects. Subjective symptom assessment revealed increased total obstructive scoring (p < 0.05). However, irritative symptom indexes in patients with diabetic cystopathy were not different from those of the normal subjects. Although uroflowmetry and the AUA Symptom Index are not specific to identify detrusor impairment, these methods appeared to be useful to decide upon further urodynamic investigations.

Age Factors↗

PubMedDate not supplied

Sutureless vasovasostomy using new vascular closure staples in rats.

To evaluate the efficacy of sutureless vasovasostomy using vascular closure staples, 20 male Sprague-Dawley rats were used for the study. In each rat, a sutureless vasovasostomy using vascular closure staples was performed on a randomly selected side, and a conventional end-to-end microsutured anastomosis was performed on the opposite side. Operation time was significantly shorter for sutureless vasovasostomy using vascular staples (p < .001). Four weeks after the surgery, however, patency rate and granuloma formation were not different for conventional end-to-end vasovasostomy vs. sutureless vasovasostomy. These findings suggest that sutureless vasovasostomy is simple to perform and requires fewer special technical skills. This procedure may provide an alternative to the conventional vasovasostomy.

Animals↗

PubMedDate not supplied

Reevaluation of testicular biopsies of males with nonobstructive azoospermia in assisted reproductive technology.

Spermatogenesis was investigated in seminiferous tubules of 100 males with nonobstructive azoospermia. Forty-four (44%) cases had Sertoli-cell-only syndrome, 23 (23%) had spermatogonium in the tubules, 17 (17%) had primary spermatocyte in the tubules, and 16 (16%) had round or late spermatid in the tubules. No cases showed secondary spermatocyte present in the tubules. The mean serum follicle-stimulating hormone (FSH) in males with nonobstructive azoospermia was significantly higher than that in males with obstructive azoospermia (p < .001). The mean concentrations of serum FSH in cases with Sertoli-cell-only syndrome and spermatogonium in the tubules were significantly higher than those in cases with primary spermatocyte and spermatid in the tubules (p < .05-.001). The results indicate that the evaluation of testicular histology using the type of germ cells present in seminiferous tubules is available for assisted reproductive technology.

Biopsy↗

PubMedDate not supplied

Effect of testosterone replacement therapy on serum PSA in patients with Klinefelter syndrome.

The effect of testosterone replacement therapy on serum prostate-specific antigen (PSA) was investigated in 11 patients with Klinefelter syndrome. Significant increases in serum testosterone level and prostate volume were observed after testosterone replacement therapy. However, serum PSA level did not change after testosterone replacement therapy. It would appear that serum PSA is not influenced by exogenous testosterone in patients with Klinefelter syndrome.

Adult↗

PubMedDate not supplied

Results and complications of laparoscopic Palomo varicocelecctomy.

The laparoscopic Palomo varicocelectomy were performed in 38 males with left-sided varicocele. The mean operation time was 37 (25-56) min. There were no intra-abdominal visceral or vascular complications during operation. Neither testicular atrophy nor recurrence was observed postoperatively. However, hydrocele formation was found in two (5.3%) patients. These findings suggest that laparoscopic Palomo varicocelectony is a safe and effective procedure for patients with varicocele.

Adolescent↗

PubMedDate not supplied

Successful treatment of retrograde ejaculation with amezinium.

The effect of amezinium, a new type of antihypotensive agent, on retrograde ejaculation was evaluated in 3 patients with retrograde ejaculation. The patients received 10 mg amezinium orally once a day. All patients achieved antegrade ejaculation. Semen analyses revealed 6-50 x 10(6)/mL (mean 28.7 x 10(6)/mL) sperm with a motility of 20-50% (mean 36.7%). The wives of 2 patients became pregnant within 6 months of the initial treatment. None of the patients had any side effects. It would appear that amezinium is a useful treatment for retrograde ejaculation.

Adult↗

PubMedDate not supplied

Round spermatid transfer and embryo development.

With the advent of assisted reproductive technology, pregnancy has become possible without sperm. The nucleus of the round spermatid, which contains a complete haploid set of chromosomes, can be used as a substitute for spermatozoa in animals and humans. In the mouse, round spermatids from surgically induced cryptorchid and prepubertal testes can fertilize normally and develop into normal offspring. The spermatid nucleus develops into a large pronucleus only when the oocyte is activated by artificial means, such as electrostimulation and oscillogen injection. Nuclei of mouse primary spermatocyte have been injected into oocytes and found to undergo meiosis, form an embryo and produce live young. Intracytoplasmic injection of male immature germ cells may become available as a treatment for patients with azoospermia due to maturation arrest.

Animals↗

PubMedDate not supplied

Circulating levels of inhibin in hemodialysis males.

Serum concentrations of LH, FSH, testosterone, and inhibin were measured in 65 hemodialysis male patients and 60 healthy men. In hemodialysis patients, serum levels of LH, FSH, and inhibin were significantly elevated compared to the controls (p < 0.001). However, serum testosterone level was significantly reduced in hemodialysis males (p < 0.01). Neither serum LH nor FSH correlated with inhibin in the control and hemodialysis males. In hemodialysis patients, serum inhibin showed a positive correlation with serum creatinine (p < 0.05). It would appear that an elevated level of serum inhibin is caused by a decreased metabolic clearance rate or testicular dysfunction due to chronic renal failure, and indicates the abnormality of hypothalamic-pituitary-testicular axis leading to hypergonadotropic hypogonadism in hemodialysis males.

Adult↗