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Biomedical subjects

O Hetland

Publications and source records attributed to O Hetland.

36 records · Page 2Linked to original sources
PubMed1982

In vitro effect of phospholipase C from Bacillus cereus on tissue thromboplastin from different species.

Purified phospholipase C from Bacillus cereus caused a significant loss in the procoagulant activity of thromboplastin preparations from man, rabbit, sheep, cow, rat and mouse. However, marked differences were observed with respect to the degree of inactivation. Rat, mouse, bovine and one type of rabbit preparations (prepared from acetone powdered brain) were markedly more sensitive to attack by phospholipase C than were preparations of human, sheep and standard rabbit preparations. The relative amounts of the individual phospholipids in thromboplastin preparations showed only minor variations among the species. The effect of phospholipase C on each of these phospholipids in the various thromboplastin preparations showed some significant differences.

Animals↗
PubMed1982

Synthesis of thromboplastin protein by a murine macrophage-like cell line.

The established murine macrophage cell line J-774.1 responds to endotoxin with synthesis of thromboplastin apoprotein. The response develops in the absence of added lymphocytes and there is no increased responsiveness in cocultures of J-774.1 cells and BALB/c lymphocytes. J-774.1 cells therefore do not depend on lymphocyte cooperation for their response to endotoxin.

Animals↗
PubMed1982

Phospholipase C from Bacillus cereus has sphingomyelinase activity.

Purified, electrophoretically homogeneous phospholipase C (PLC) preparations can be separated into two peaks by isoelectric focusing in sucrose gradients. The main peak has an isoelectric pH of 6.6-6.8 and contains two Zn2+ per molecule. The more acid peak (isoelectric pH about 6.2) contains about one Zn2+ per molecule and has a markedly reduced specific activity which can be raised by adding Zn2+. The purified enzyme has a low sphingomyelinase activity which coincides completely with the lecithinase activity in fractions from isoelectric focusing. The sphingomyelinase activity was greatly enhanced by substitution of Co2+ for Zn2+ but remained essentially unaltered when the levels of Ca2+ and Mg2+ were changed. These findings provide evidence that the sphingomyelinase activity is a true endogenous activity of PLC and not caused by contaminating sphingomyelinase.

Bacillus cereus↗
PubMed1982

Toxicity of phospholipase C in rabbits.

Tissue thromboplastin is the most potent physiological trigger of blood coagulation and is probably involved in the pathogenesis of several forms of intravascular coagulation. Phospholipase C from Bacillus cereus is an effective inhibitor of thromboplastin. As part of an investigation into the possible use of phospholipase C as a therapeutic or prophylactic agent in thrombosis and other forms of intravascular coagulation, we have previously studied its effect in rats. We now report on the toxicity in rabbits (along with some data on cats and monkeys). Estimated LD50 for rabbits was about 0.45-0.65 mg/kg (as compared to 1.70 mg/kg for rats). No respiratory or circulatory changes were observed, but phospholipase C caused a significant increase of several plasma enzymes in rabbits.

Acid-Base Equilibrium↗
PubMed1980

Assessment of cerebral injury with spinal fluid creatine kinase (CSF-CK) in patients after cardiac resuscitation.

Serial determinations of creatine kinase in cerebrospinal fluid (CSF) were made in eighty-two patients, that were comatose after cardiac resuscitation because of ventricular fibrillation. In all patients who remained unconscious creatine kinase (CSF-CK) increased from less than 2 U/L to 11 U/L or more with maximum CSF-CK averaging 53 +/- 6 U/L 48-72h after resuscitation. In patients who recovered consciousness maximum CSF-CK activity never exceeded 11 U/L. However, maximum CSF-CK activity between 5 and 11 U/L was always associated with some permanent cerebral dysfunction. Complete cerebral restitution was only observed when maximum CSF-CK was 5 U/L or less. In some patients a maximum CK activity of 4 U/L was associated with depressed memory functions. The blood-brain barrier was impermeable to CK as evidenced by isoenzyme analysis and lack of correlation between CK activity in CSF and peripheral blood. CSF-pressure rose slightly to 217 +/- 11 mmH2O in patients with CSF-CK above 10 U/L and to 197 +/- 15 mmH2O when CSF-CK was below 5 U/L. The results indicate that high CSF-CK activity, whether appearing early or late after successful cardiac resuscitation, effectively predicts an unfavourable outcome of global cerebral ischemia. No rise in CSF-CK was always indicative of full cerebral recovery.

Aged↗
PubMed1978

Plasma hypoxanthine levels in pigs during acute hypoxemia. A correlation between lactate and base deficit concentrations.

Tissue hypoxia was induced in pigs by artificial ventilation with 6% O2 in N2 for 18 min. Base deficit, lactate and hypoxanthine increased linearily during this period, and were significantly higher than initial values in the course of 6 min of hypoxemia. High correlation coefficients between hypoxanthine and lactate (mean 0.98) and between hypoxanthine and base deficit (mean 0.98) were found. During the recovery period when the animals were ventilated with air, high correlation coefficients between lactate and hypoxanthine (mean 0.75) and between base deficit and hypoxanthine (mean 0.83) were also found. It is concluded that plasma hypoxanthine concentrations should be used to assess tissue hypoxia also in clinical situations.

Animals↗
PubMed1977

Activation of creatine kinase activity in lyophilized control materials.

The activity of creatine kinase in lyophilized control materials has been studied. In most such samples the activity was highly dependent upon time of exposure to the reagent system including the thiol activator, increasing pre-incubation time gave rise to increasing activities of creatine kinase. The increase of the activity could be prevented by reconstitution in presence of a thiol activator. In this way stable control materials was obtained for use in several days.

Creatine Kinase↗
PubMed1975

The heterogeneity of the serum activity of gamma-glutamyl transpeptidase in hepatobiliary diseases as studied by agarose gel electrophoresis.

Using electrophoresis on agarose gel, the serum activity of gamma-GT in various hepatobiliary diseases has been separated into several electrophoretic fractions. Predominance of activity was always found in fractions corresponding to either alpha1-, alpha2- or beta-globulins. The predominant activity fractions have been related to the various disease states and to the zymogram patterns of gamma-GT found in bile and tissue homogenates of liver, bile duct and pancreas. Bile disclosed activity in the beta-region, tissue homogenates of liver mostly in the alpha1- and alpha2- areas, bile dict showed very faint activity in the alpha1- position, while extracts of pancreas revealed activity in the regions of alpha2, alpha2-beta and beta-gamma. In serum, dominance of alpha1-activity was found in all cases associated with alcoholism and alcoholic cirrhosis, this activity also appeared as the most frequent dominant one in biliary obstruction. Predominance of alpha2 and/or the beta-fraction in most cases occurred associated with malignant processes involving liver and/or pancreas.

Alcoholism↗