Biomedical subjects

O Hatase

Publications and source records attributed to O Hatase.

At least 91 records · Page 5Linked to original sources

PubMed1979

Partial purification and biological activities and properties of chick growth factors.

Cellular stimulating factors on cell proliferation in the supernatants of chick embryo carcases and adult muscles were studied. There were plural stimulating factors in embryonic and adult muscular supernatants that promoted cell proliferation without any supplement of sera and other materials. Salting-out methods with ammonium sulfate, ethanol fractionation, and isoelectric precipitation were used to isolate the stimulating factors, and these three methods proved the presence of plural stimulants on cell proliferation in the supernatants of chick embryo and adult muscles. The stimulants had altered physico-chemical properties and biological activities due to embryological development. The embryonic stimulants enhanced the synthesis of DNA and protein remarkably, and RNA synthesis in whole cell systems slightly. The muscular stimulants enhanced protein synthesis without any stimulation of DNA and RNA synthesis. Partial purification of the stimulants from the ethanol fractions was performed by DEAE-cellulose chromatography and Sephadex gel chromatography.

Animals↗

PubMed1977

Plural growth factors in the supernatant of embryos and adult muscles of chickens.

An attempt was made to isolate the cell proliferation stimulation factors in the supernatant of embryo carcases and adult muscles of chickens. Evidence was obtained for the presence of at least two or more stimulating factors in both the embryonic and adult muscular supernatants. These factors did not require a supplement of sera or other supporting agents. Furthermore, the use of the salting-out method with ammonium sulfate revealed two or more growth stimulants in the supernatant of chick cells.

Animals↗

PubMed1977

Role of hydrophobic interaction in hapten-antibody binding.

The precipitation reaction of bovine serum albumin coupled with p-azophenylleucine with homologous antibody was inhibited by several structurally related haptens. The isobutyl group substituent on alpha-carbon atom of the leucine residue contributed more than -5.8 Kcal/mol to the free energy of binding. This value was consistent with the free energy change expected from the transfer of n-butane from an aqueous environment to liquid n-butane. The observed contribution was explained, in terms of the hydrophobic interaction of the isobutyl group with the antigen binding site of the antibody molecule. These results were also compared with other hapten-antibody systems.

Animals↗

PubMed1977

Mitochondrial sulfhydryl groups. A possible endogenous probe of conformational changes in the mitochondrial membrane.

The protein-bound sulfhydryl (SH) groups of the mitochondrial membrane were determined with Ellman's reagent in energized and non-energized configurational states of mitochondria and submitochondrial particles. When beef heart mitochondria were energized by respiration, there was a decrease in titratable protein-bound SH groups which varied according to substrate: NADH-linked substrates induced a decrease of about 10 nmol per mg of protein,succinate about 7, and ascorbate-tetramethyl-p-phenylene-diamine about 3. Similar changes occurred in phosphorylating submitochondrial particles. A decrease in SH titer was also observed in non-energized conditions, induced by hypotonic treatment and by some reagents inhibiting electron transport and oxidative phosphorylation and inducing orthodox configuration. These changes in protein-bound SH groups might be useful in analyzing the conformational states of mitochondrial membranes.

Animals↗

PubMed1976

The presence of phosphate-binding protein in inner mitochondrial membrane.

Phosphate-binding protein(s) was found in the inner mitochondrial membrane of calf heart by Sephadex G-200 and G-25 gel filtration. The binding activity was inhibited by N-ethylmaleimide and competed by a large amount of cold phosphate. The amount of phosphate bound to the fraction was 29 nmoles per mg of protein. Affinity chromatography with phosphate-bound Sepharose 4B confirmed the presence of phosphate-binding protein(s) in the active fraction of mitochondrial membrane fractionated by gel filtration.

Carrier Proteins↗

PubMed1976

Cell proliferation stimulating factors in the supernatant of embryos and adult muscles of chickens of the same strain.

Stimulating factors of cell proliferation were extracted from the supernatant of embryos and adult muscles of chickens of the same strain. The stimulating activity and physicochemical properties of these factors were altered after embryological development. The embryonic factors were about 3.5 times more effective than the muscular factors. These factors might be useful in analysis of the regulatory mechanisms of cell proliferation.

Animals↗

PubMed1976

Changes in the activities of nucleoside triphosphatases and nucleoside kinases in Vero cells infected with Simian virus 40.

Changes in the activities of three enzymes involved in nucleotide metabolism were studied following infection of Vero cells with SV40. The results showed that SV40 infection enhanced the activities of uridine phosphokinase and UMP phosphokinase involved in nucleotide synthesis and suppressed the activity of nucleoside triphosphatases involved in nulceotide degradation. These SV40-induced enzyme activity changes probably served to somehow increase the quantity of nucleotides for virus proliferation.

Animals↗

PubMed1975

Avian myeloblastosis virus-induced lymphosarcoma producing erythroblastic leucosis in chicks.

Acute myeloblastosis and several forms of tumor, including one case of lymphosarcoma occurred when avian myeloblastosis virus (BAI-A strain) was inoculated into newly hatched chicks (SPF). The homogenate of lymphosarcoma inoculated intraperitoneally into other newly hatched chicks induced a high incidence of erythroblastic leucosis. Electron microscopy did not reveal the presence of C-type virus particles in the tumor tissue. The relationship between avian myeloblastosis virus, lymphosarcoma and erythroblastic leucosis is discussed.

Animals↗

PubMed1972