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Biomedical subjects

O Hansson

Publications and source records attributed to O Hansson.

At least 55 records · Page 3Linked to original sources
PubMed1988

Site-directed mutagenesis in photosystem II of the cyanobacterium Synechocystis sp. PCC 6803: Donor D is a tyrosine residue in the D2 protein.

The chemical nature of electron donor(s) in photosystem II in photosynthetic membranes was analyzed by site-directed mutagenesis of the gene encoding the protein D2 of the photosystem II reaction center. Mutation of the Tyr-160 residue of the D2 protein into phenylalanine results in the disappearance of the electron paramagnetic resonance signal II(S) originating from D(+), the oxidized form of the slow photosystem II electron donor D. Signal II(S) is still present if a neighboring residue in D2, Met-159, is mutated into arginine. Both mutants have normal rereduction kinetics of the oxidized primary electron donor, P680(+), in octyl glucoside-extracted thylakoids, indicating that D is not directly involved in P680(+) reduction. However, overall photosystem II activity appears to be impaired in the Tyr-160-Phe mutant: photosystem II-dependent growth of this mutant is slowed down by a factor of 3-4, whereas photoheterotrophic growth rates in wild type and mutant are essentially identical. Binding studies of diuron, a photosystem II herbicide, show that there is no appreciable decrease in the number of photosystem II centers in the Tyr-160-Phe mutant. The decrease in photosystem II activity in this mutant may be interpreted to indicate a role of D in photoactivation, rather than one as an important redox intermediate in the photosynthetic electron-transport chain.

Journal Article↗
PubMed1988

Impressions for prosthodontic restorations reproducing narrow spaces and severe undercuts.

Distortion of an impression material may occur in special clinical situations in which the material's elastic limit is exceeded. Impressions were made of a master model designed to test such situations. Three different hydrocolloids and an addition silicone were used in the experiment. An extended setting time had to be used for one material, to prevent it from tearing. When this extended time was used, this material was the dimensionally most accurate material in the test. All materials were accurate in reproducing normally positioned abutments, while some of the impressions failed to reproduce adjacent abutments with particularly narrow interspaces; narrow passages may therefore require further tooth preparation. In the most severely undercut area differences existed among the materials, and sometimes tears occurred. When the papilla is missing, the undercut area should be blocked out to prevent plastic deformation of the impression material, causing inaccurate restorations.

Agar↗
PubMed1985

Casting accuracy of a nickel and beryllium-free cobalt-chromium alloy for crown and bridge prostheses and resin-bonded bridges.

In the 1970's economic factors dictated the development of alternatives to gold alloys in dentistry in the USA and in Europe. A similar development has not occurred in Sweden because of different laws. Alloys that contain nickel and beryllium present a health hazard and are therefore of little interest to the Swedish market. A review of the literature shows that castings of base-metal alloys are less accurate than castings of conventional gold alloys and of low gold alloys. However, in long-span-bridges and in thin resin-bonded cast restorations, their physical and mechanical properties are superior to those of the gold alloys. In this study the casting accuracy of a nickel- and beryllium-free cobalt-chromium alloy, Neobond II Special, is investigated. Neobond II Special was found to be less accurate than Sjödings C-guld. The marginal discrepancies of the castings were small, however, when the castings were oversized. It also proved to be technique sensitive to conventional dental laboratory procedures. Thus, it seems difficult to get castings with an acceptable retention as well as small marginal discrepancies when using the base-metal alloy.

Chemical Phenomena↗
PubMed1984

EPR studies on the photosystem II donor side in salt-washed and reconstituted inside-out thylakoids.

EPR measurements on inside-out thylakoids revealed that salt-washing, known to inhibit oxygen evolution and release a 23 and a 16 kDa protein, induced a Signal IIf and decreased the EPR signal from state S2. Readdition of the released 23 kDa protein restored the oxygen evolution and decreased the Signal IIf, but did not relieve the decrease in the state S2 signal. It is suggested that salt-washing inhibits the electron transfer from the oxygen-evolving site to Z, the physiological donor to P680. In inhibited photosystem II units lacking Signal IIf, Z+ is rapidly reduced, possibly by a modified S-cycle unable to evolve oxygen.

Darkness↗
PubMed1984

A historical review of hydrocolloids and an investigation of the dimensional accuracy of the new alginates for crown and bridge impressions when using stock trays.

Reversible hydrocolloids have been used since 1937 and irreversible hydrocolloids since 1947 for the making of impressions for fixed prostheses; the impression techniques were similar to today's. The dimensional accuracy of reproduction of the irreversible hydrocolloids has been proved since 1947. In this paper the dimensional accuracy of three new alginates, Algi -X ( Algiflex Super, Howmedica Alginate), Ardent Alginate, Ultrafine and a combination material, Colloid 80/ Algiace ( Dentloid / Algiace ) were compared to two agar hydrocolloids and an addition silicone, when different stock trays were used as in the dental clinic. In most clinical situations the new alginates used in metal stock trays are as accurate as the "old" impression materials. The combination material is less accurate when many abutment preparations are to be reproduced. Whether perforated or nonperforated metal stock trays are used with the alginates is of no consequence to the accuracy. Alginates used in disposable plastic trays may cause severe inaccuracy. In narrow spaces with severe unblocked undercuts some of the alginates may be inferior to the other impression materials in this study.

Agar↗
PubMed1982

The structure of the paramagnetic oxygen intermediate in the cytochrome c oxidase reaction.

A paramagnetic intermediate with an unusual e.p.r. spectrum is formed when fully reduced cytochrome c oxidase is allowed to react with dioxygen at 173 K. The effect on the e.p.r. spectrum of using dioxygen enriched in 17O was investigated. These experiments show that an oxygen atom derived from dioxygen is bound to Cu2+ in the intermediate. The e.p.r. parameters can be explained in terms of a weak antiferromagnetic interaction (J approximately equal to 10 cm-1) between Cu2+B and cytochrome a3 in the low-spin ferryl ion state. It is suggested that an OH- ion bound to Cu2+B is hydrogen bonded to the oxygen atom of the ferryl ion in cytochrome a3.

Animals↗