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Biomedical subjects

O Guerci

Publications and source records attributed to O Guerci.

At least 37 records · Page 2Linked to original sources
PubMed1981

Separation of human bone marrow cells by sedimentation.

Sedimentation at unit gravity of human bone marrow cells, for 15 h at 4 degrees C on linear density gradient of Ficoll in culture medium ranging from 1.020 to 1.065 g/ml shows that a differential migration of the bone marrow cell sub-populations exists with precise mean densities 1.021 +/- 1 x 10(-3) g/ml for lymphocytes; 1.024 +/- 2.5 x 10(-3) g/ml for non-eosinophil granulocytes; and 1.055 +/- 10 x 10(-3) g/ml for metamyelocytes; 1.030 x 3.5 x 10(-3) g/ml for other myeloid cells (myeloblasts, promyelocytes, myelocytes); 1.040 +/- 1.040 +/- 3 x 10(-3) g/ml for eosinophil granulocytes; and 1.055 +/- 10 x 10(-3) g/ml for megakaryocytes. The highest percentages of S phase cell and G2 and M phase cells determined by a cytofluorograph correspond to the peaks of immature myeloid cells (myeloblasts, promyelocytes and myelocytes). This method of bone marrow cell separation may be used to study the cell cycle in pathological bone marrows (leukaemia in particular) and to determine the effects and the efficiency of some antimitotics.

Bone Marrow Cells↗
PubMed1981

[Application of sedimentation at 1 g on a Ficoll gradient to the separation of bone marrow cells].

Sedimentation at unit gravity of human bone marrow, during 15 hours at 4 degrees C on a linear density gradient of Ficoll in culture medium ranging from 1.020 to 1.065 g/ml shows that it exists a differential migration of bone marrow cells subpopulation with a precise mean densities : we find successively : 1.021 +/- 1.10(-3) g/ml for the lymphocytes, 1.024 +/- 2.5.10(-3) g/ml for the non eosinophil granulocytes, 1.025 +/- 2.5.10(-3) g/ml for the metamyelocytes, 1.030 +/- 3.5.10(-3) g/ml for the immature myeloid cells (myeloblasts, promyelocytes, myelocytes), 1.040 +/- 3.10(-3) g/ml for the eosinophil granulocytes, 1.055 +/- 10.10(-3) g/l for the megakaryocytes. The highest percentages of S phase cells, G2 and M phase cells determinated with a cytofluorograph correspond to peaks of immature myeloid cells (myeloblasts, promyelocytes and myelocytes). This method of bone marrow cells separation may be used to study the cell cycle in pathological bone marrows (leukaemia in particular) and to determine the effects and the efficiency of some antimitotics.

Bone Marrow Cells↗
PubMed1980

[Pericarditis as the presenting manifestation of acute monoblastic leukemia-Report of a case and review of the literature (author's transl)].

A case of acute monoblastic leukemia in a 68-year-old man is reported in which one of the presenting manifestations was a pericardial effusion. A blood-stained fluid was removed from the pericardium. The pericardial fluid showed blast cells on microscopical examination. The patient was treated with 6-Mercaptopurine and Methotrexate and achieved a short partial remission. He died of general infection, 4 months after the start of his illness. We found in the medical literature 30 additional cases with a mean age of 24 +/- 16 years and a striking male predominance (68. p. 100). We found 15 acute lymphoid leukemias, 9 acute myeloid leukemias, 4 acute indifferentiated leukemias, and 2 acute nontyped leukemias. The leukemic pericardial involvement is often associated with splenomegaly, adenopathy and pleural effusion. The management procedures include pericardiocentesis, general of local chemotherapy, irradiation of the cardiac area. The prognosis is generally poor, with a mean survival of 5 months.

Acute Disease↗