One hundred and fifty-nine prompt lactose-fermenting strains of Enterobacteriaceae were tested for H(2)S production, ornithine decarboxylase activity, motility, and citrate utilization by using two different sets of test media. It was shown that the three tests first mentioned could be carried out in a single tube, the results being as reliable as when each was done separately. Simmons medium was preferred for the detection of citrate utilization. Since these four tests require only two tubes, they are considered more convenient in routine diagnostics. A combined medium for the detection of H(2)S production and motility is described.

Bacteriological Techniques↗

PubMed1970

[Evaluation of a dip-slide method for bacteriological examination of urine].

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Bacteriological Techniques↗

PubMed1968

Rapid identification of small numbers of bacteria after short-time cultivation in the presence of FITC--conjugated antiserum: report on a method.

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Bacteria↗

PubMedDate not supplied

Fatty acid and polar lipid analysis as tools in the identification of Mycobacterium leprae and some related slow-growing mycobacterial species.

Some species of slow-growing Mycobacteria, including Mycobacterium leprae (1 strain), M. lepraemurium (2 strains), M. paratuberculosis (12 strains) and a group of 12 M. avium-like strains (isolates from wild animals) were examined by gas chromatography (GC) for cellular fatty acids and by thin-layer chromatography (TLC) for polar lipids. All the GC patterns, including that of M. leprae, contained high levels of tuberculostearic-, stearic-, octadecenoic- and palmitic acid. Tetradecanoic-, pentadecanoic-, hexadecenoic- and heptadecanoic acid were also generally present but in lower concentrations. In addition to these acids shared by all strains, each bacterial species or group was found to exhibit compounds which were not detected (or detected in considerably lower quantities) in the other taxa examined. Thus each bacterial species or group could be distinguished by their GC profiles. The corresponding TLC patterns were also rather complex. A total of 39 different spots were distinguished. A few of these were shared by all strains, some were characteristic of certain species or groups, whereas others were strain-specific. Both M. leprae and M. lepraemurium shared several features with the other strains but could be distinguished from each other and from the others by their patterns of slow-moving (polar) spots. The 12 M. avium-like strains were divided into two main groups, one with only a few slow-moving spots (rough stains), and one with several slow-moving spots (smooth strains) which included the M. avium reference strains.

Chromatography, Gas↗