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Biomedical subjects

O Alvarez

Publications and source records attributed to O Alvarez.

At least 73 records · Page 4Linked to original sources
PubMed1978

Voltage-dependent capacitance in lipid bilayers made from monolayers.

Electrocompression has been measured in lipid bilayers made by apposition of two monolayers. The capacitance C(V), as a function of membrane potential, V, was found to be well described by C(V) = C(O) [1 + alpha(V + delta psi)2] where C(O) is the capacitance at V = O, alpha is the fractional increase in capacitance per square volt, and delta psi is the surface potential difference. In lipid bilayers made from monolayers alpha has a value of 0.02 V-2, which is ca. 500-fold smaller than the value found in solvent containing membranes. In asymmetric bilayers made of one neutral and one negatively charged monolayer, delta psi values were found to be those expected from independent measurements of surface charge density. If the fractional increase in capacitance found here is a good approximation to that of biological membranes, nonlinear capacitative charge displacement derived from electrostriction is expected to be less than 1% of the total gating charge displacement found in squid axons.

Electrochemistry↗
PubMed1978

Enzootic and epizootic Venezuelan equine encephalomyelitis virus in horses infected by peripheral and intrathecal routes.

Forty-five horses were infected peripherally or intrathecally with enzootic or epizootic strains of Venezuelan equine encephalomyelitis (VEE) virus. Low titers of virus appeared in cerebrospinal fluid (CSF) after peripheral inoculation of enzootic or epizootic VEE virus strains. Intrathecal infection with either epizootic or enzootic VEE virus produced higher titers of virus in CSF than did peripheral infection. In contrast to peripheral infections with enzootic strains, intrathecal infections with these strains caused death. The animals that died had widespread histopathologic changes and large amounts of virus in brain tissue. The attenuated VEE virus vaccine strain, TC-83, also multiplied in the brain of horses inoculated intrathecally but caused no clinical disease and little histopathologic damage.

Animals↗
PubMed1978

[An endemic area of sporotrichosis in Guatemala].

An endemic area of sporotrichosis is described in the Lake of Ayarza District, South Guatemala, where 53 patients have been observed within 3 years. In 45.3% of the cases, the infection appeared after handling fish. The disease was more frequently observed in man (83%) and in patients less than 30 years of age. The most frequent clinical type was the ascending lymphocutaneous sporotrichosis of the limbs. Some cases of ulcerative or verrucous lesions were seen. Almost all the patients cured rapidly either by potassium iodide (46 patients) or spontaneously, or after application of local heat (4 patients). Intradermal tests were performed in healthy population in the endemic area and, for comparison, in Guatemala City. Whole yeast cell antigens of Sporothrix schenckii and Ceratocystis stenoceras were used in these tests. Skin tests to both antigens were more frequently positive in the endemic area; the highest frequency was obtained with the antigen of C. stenoceras. A serological study was performed in 26 patients. The fluorescent antibody staining technique was more sensitive than yeast cell and latex particles agglutinations. C. stenoceras was isolated from bark of some trees, especially Eucalyptus, in the environment, but not S. schenckii.

Agglutination↗
PubMed1975

The nature of the voltage-dependent conductance of the hemocyanin channel.

The electrical responses of individual hemocyanain channels in oxidized cholesterol membranes demonstrate that the voltage-dependent conductance of many-chanel membranes arises from two different mechanisms. These are the voltage-dependent redistribution of channels among several discrete single-channel conductance states themselves. The relaxation time for the discrete conductance changes is of the order of seconds nd the relaxation time of the continuous conductance changes is of the order 10(-4) seconds. As salt concentration in the bathing medium is increased, the single-channel conductance first increases lineary and then saturates. The characteristics of the saturation curves suggest that the continuous conductance changes occur at the edges of the channel and that the mean time an ion spends in the channel is 4 nanoseconds...

Cholesterol↗
PubMed1975

Voltage-dependent conductance induced by hemocyanin in black lipid films.

When hemocyanin is added to a black lipid film, the conductance increases in discrete steps. For negative potentials the single step conductance is constant, but for positive potentials the step conductance appears to decrease as the potential increases. At high positive potentials the conductance fluctuates between several levels. These data suggest that, in lipid membranes, hemocyanin conducts ions through discrete channels. The voltage-dependent conductance observed at high levels of conductance seems to be a consequence of the properties of the conductance of the single channel.

Binding Sites↗
PubMed1975

Kinetic characteristics of the excitability-inducing material channel in oxidized cholesterol and brain lipid bilayer membranes.

The kinetic characteristics of the opening and closing of the excitability-inducing material (EIM) channel in oxidized cholesterol and in brain lipid bilayers are compared. The kinetics of the opening and closing of individual ion-conducting channels in bilayers doped with small amounts of EIM are determined from discrete fluctuations in ionic current. The kinetics for approach to steady-state conductance are determined for lipid bilayers containing many channels. Steady-state and kinetic characteristics for the EIM channel incorporated in brain lipid bilayers can be accounted for by the model developed for the EIM channel incorporated in oxidized cholesterol membranes. Relaxation time, calculated from rate constants of single-channel membranes or directly measured in many-channel membranes is strongly temperature dependent, and is always shorter in brain lipid membranes. Changes in temperature do not affect the interaction of the electric field and the open channel, but the open configuration of the EIM channel in brain lipid bilayers is stablized with increasing temperature. The configurational energy difference between the open and closed channel, calculated from temperature studies, is larger in brain lipid bilayers. The energy barrier which separates the two configurations of the channel is larger in oxidized cholesterol bilayers.

Alamethicin↗
PubMed1972

Experimental infection of horses with an attenuated Venezuelan equine encephalomyelitis vaccine (strain TC-83).

Ten horses (Equus caballus) were vaccinated with strain TC-83 Venezuelan equine encephalomyelitis (VEE) virus vaccine. Febrile responses and leukopenia due to a reduction of lymphocytes and neutrophils were observed in all animals. Viremias were demonstrable in eight horses, with a maximum of 10(3.5) median tissue culture infectious dose units per ml of serum in two horses. Clinical illness with depression and anorexia were observed in five horses. Neutralizing (N), hemagglutination-inhibiting, and complement-fixing antibodies to the vaccine virus were demonstrable by 5, 6.5, and 7 days, respectively, after vaccination. Differential titrations of serum to six VEE strains revealed high titers of N antibody to vaccine virus, moderate titers to the epizootic Trinidad donkey no. 1 strain (VEE antigenic subtype I, variant A) from which TC-83 was derived, and low titers to two other epizootic strains (subtype I, variants B and C) in all horses at 1 month after vaccination; some animals responded with low levels of N antibody to the enzootic viruses (subtype I, variants D and E). Fourteen months after vaccination, six animals with detectable N antibody were challenged with MF-8 (subtype I, variant B), an epidemic-epizootic strain isolated in 1969 from a man in Honduras. All horses resisted challenge with the equine pathogenic strain of VEE. Marked increases of N antibody in most horses were demonstrable to some VEE strains when tested 1 month after challenge.

Animals↗
PubMedDate not supplied

Axis selection in transverse magnetic resonance imaging of the brain: electronic angulation techniques.

The cylindrical design of most head coils utilized with current magnetic resonance (MR) imaging units and the necessity of close approximation of the coil to the head to maximize signal-to-noise ratio precludes flexion or extension of the head to any significant degree during of imaging of the brain. For this reason, the canthomeatal line is approximately parallel to the standard transverse magnetic axis. Standard computed tomography (CT) scans in the transverse plane are usually obtained at an approximately 25 degrees angle to Reid's baseline (RBL). This leads to projection differences in viewing and comparing standard transverse MR and CT studies. High convexity lesions which may present anteriorly on a given CT section may present posteriorly on an MR section which appears to be at a comparable level on first inspection. Secondly, one or more transverse MR sections usually display a portion of the occipital lobes behind the cerebellar hemispheres. The region of the tentorium and straight sinus can occasionally give rise to a vermiform appearance (the "AVM artifact"). Thirty patients were studied with MR at a 20-30 degrees angulation to RBL without any loss of image quality and with excellent visualization of the posterior fossa. The effect of transverse axis change on lesion position was demonstrated in five high convexity lesions and by utilizing fixed brain specimens. While clearly MR can accurately localize lesions utilizing orthogonal multiplanar techniques, it is suggested that investigators and clinicians currently performing MR studies of the brain consider potential advantages of electronic angulation techniques for comparative clinical studies and certain research applications.

Brain↗
PubMedDate not supplied

Even echo MR rephasing in the diagnosis of giant intracranial aneurysm.

Blood flow magnetic resonance (MR) imaging has played a significant role in the evaluation and differentiation of vascular from nonvascular lesions, particularly as it applies to the CNS. Even echo rephasing and high velocity signal loss are two phenomena that are useful in differentiating primary vascular lesions that exhibit either slow laminar flow or rapid flow from solid masses or clot-containing vascular lesions. Application of these two basic principles of blood flow imaging with MR aided in establishing the correct diagnosis in a case of giant intracranial aneurysm.

Aged↗
PubMedDate not supplied

Multilevel thoracic disk herniations: CT and MR studies.

Thoracic disk herniation is a disorder that can present clinically perplexing problems for physicians. The true incidence of thoracic disk herniation is difficult to assess with various authors reporting an incidence ranging between 0.15 and 1.8% of all disk herniations. Multiple thoracic disk herniations are rare and, to the best of our knowledge, have received little attention in the orthopedic, neurosurgical, and radiological literature. A retrospective review of 680 myelograms performed at our institution was carried out and only three cases of multilevel thoracic disk herniations were found. We analyze these cases, discuss the relative value of the imaging modalities used in their diagnosis, and review the literature dealing with this interesting disorder.

Adult↗