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Biomedical subjects

N de Tribolet

Publications and source records attributed to N de Tribolet.

At least 217 records · Page 12Linked to original sources
PubMed1982

[Transthoracic approach to dorsal disc hernias].

Three cases of thoracic disc protrusions, treated by the transthoracic surgical approach, are reported. The signs and symptoms of the patients were not essentially different from findings encountered in other types of space-occupying thoracic intra-spinal lesions. The diagnosis by high resolution computed axial tomography provides more information and may eliminate future use of myelography. In their median localisation, the thoracic disc protrusions are equally accessible by either a right or a left transthoracic approach. Yet, the right lateral approach was preferred because of the overlapping aorta on the left. If, for some reason, a left lateral approach is considered, the level of entry of Adamkiewicz artery should be identified by preoperative spinal angiography. The postoperative results are excellent in all three cases as opposed to a fourth operated by laminectomy.

Adult↗
PubMed1981

Characterization of an established human malignant glioma cell line: LN-18.

A human malignant glioma cell line, LN-18, has been established in monolayer culture and subcultured for more than 115 passages. LN-18 cells grow in vitro as bipolar or stellate cells with pleomorphic nuclei, have a doubling time of about 72 h and a plating efficiency of 3%. The glial nature of these cells has been assessed by ultrastructural examination. The synthesis of glial fibrillary acidic and S-100 proteins could not be demonstrated, although the initial biopsy tissue and the early cultures were positive for the former. The presence of Ia-like antigens on the surface of these cells was demonstrated using allo and xeno antisera. LN-18 cells were also shown to synthesize large quantities of fibronectin. The injection of LN-18 cells into nude mice induced the formation of solid tumor masses that could be retransplanted every 3 weeks and showed a morphology comparable to that of the initial biopsy. Karyotype analysis revealed the presence of three marker chromosomes, constantly present before and after hetero-transplantation.

Cell Division↗
PubMed1981

Presence of sex steroid hormone receptors in meningioma tissue.

Meningiomas from 10 patients were studied for the presence of sex hormone receptors. No meningioma was found to have estrogen receptors, while 4 had no receptors. The presence of progesterone receptors was independent of the age or sex of the patient, whereas androgen receptors were found only in postmenopausal women. The possible relationship between the epidemiological characteristics of meningiomas and the presence of sex hormone receptors is discussed.

Adult↗
PubMed1981

Human glioma-associated antigens detected by monoclonal antibodies.

Hybridoma cells were derived from a fusion between mouse P3x63/Ag8 myeloma cells and spleen cells from a mouse immunized with whole cells of a human malignant glioma line. Of 345 hybrids obtained, 36 secreted antibodies that reacted with the glioma cell line used for immunization as assayed by an indirect antibody-binding radioimmunoassay. After a first screening for the absence of reactivity on two nongliogenous cell lines, 3 hybrids were selected and cloned by limiting dilution. The specificity of these monoclonal antibodies was then investigated on a panel of 18 cell lines derived from human malignant gliomas, 18 cell lines from nongliogenous neoplasms, as well as normal peripheral blood lymphocytes, normal skin fibroblasts, and normal spermatozoa. The monoclonal antibodies from two positive hybrids, BF7 and GE2, reacted exclusively with glioma cells and appeared to be directed against common malignant glioma antigen(s). BF7 antibodies bound to 13 and GE2 to 17 of 18 glioma cell lines. The third monoclonal antibody, CG12, showed a broad reactivity since it bound to 10 of 18 glioma lines, five of five melanoma lines, and one of one neuroblastoma line. Absorption with normal adult and fetal brain homogenate did not modify the binding capacity of BF7 and GE2 for glioma cells, while the binding of CG12 antibodies was abolished. Reciprocal binding inhibition tests using [3H]leucine-labeled antibodies showed that BF7, GE2, and CG12 antibodies were directed against different antigenic determinants.

Antibodies, Neoplasm↗
PubMed1980

[Double suprasellar and pineal germinoma with potomania].

A case is reported of suprasellar and pineal germinoma in a 16-year-old male initially presenting with potomania without diabetes insipidus. The role of stimulation of the hypothalamus in producing pathological thirst is discussed.

Adolescent↗
PubMed1980

Evaluation of the humoral response of glioma patients to a possible common tumor-associated antigen(s).

Cytotoxic antibodies against glioblastoma-associated antigen(s) have been sought for in glioma patient sera. Complement-dependent cytotoxicity (CDC) I and antibody-dependent cell-mediated cytotoxicity (ADCC) assays were used to test sera from 80 patients using 51 Cr labelled target cells derived from eight different glioblastoma lines. As more positive sera were detected with ADCC than with CDC, ADCC assay was used for the remainder of the study. Cytotoxic antibodies were detected in the sera from 8 of 80 glioma patients (10%) by CDC and in 20 of 143 (14%) by ADCC. Fourteen percent of 27 meningioma patients and 16% of 25 normal donors used as controls were found to react in ADCC against the same glioblastoma cell lines. The positive serum samples showed extensive cross-reactions with the different glioblastoma cells, but the pattern of reactivity was different for each serum tested. The antibodies detected did not seem to be directed against tumor-associated antigen(s), since the positive sera were found to have a similar ADCC reactivity against unrelated tumor cells and normal fibroblasts. Moreover, their antiglioma reactivity was absorbed by cells of unrelated tumors and by normal platelets. These results do not support previous reports of specific humoral responses in glioma patients against common tumor-associated antigen(s).

Absorption↗
PubMed1980

Evaluation of the humoral response of meningioma patients to possible common tumour-associated antigens.

The humoral response of 39 meningioma patients to possible tumourassociated antigens was studied by means of an antibody dependent cell mediated cytotoxicity test (ADCC). Five patients were found to have cytotoxic antibodies against 1 to 10 meningioma cell lines. However, three of these were also cytotoxic against malignant glioma cell lines but not against other unrelated tumours. These results suggest the existence of surface antigens common to meningiomas and gliomas.

Adult↗
PubMed1980

Immune complexes associated with brain tumors: correlation with prognosis.

The presence of circulating immune complexes in the sera of 208 patients with intracranial space occupying lesions was assayed using the C1 q binding test. Twenty-eight percent of 96 high grade glioma patients had immune complexes as compared to 14% of 28 low grade glioma patients. The incidence of immune complexes was found to decrease in the first post-operative month. The presence of immune complexes correlated with shorter survival in a group of malignant glioma patients tested preoperatively. Nineteen patients without immune complexes had a mean survival of 8.6 months as compared to 13 patients with immune complexes who had a mean survival of 4.7 months. These results confirm similar observations in other types of malignancies.

Adult↗
PubMed1979

Familial gliomas.

The authors report two pairs of siblings who had intracranial malignant gliomas. Genetic influences in the occurence of gliomas are discussed.

Adolescent↗
PubMed1979

[Bilateral cervical internal carotid artery aneurysms (author's transl)].

Aneurysms of the cervical internal carotid artery are not rare lesions although they often remain symptomless. The authors describe the case of an eighteen-year-old boy presenting bilateral cervical internal carotid artery aneurysms of probable traumatic origin and treated conservatively. Etiology, pathogenesis, difficulties in diagnosis and choice of treatment are discussed.

Adult↗
PubMed1979

Nonsurgical treatment of interhemispheric subdural empyemas.

Interhemispheric subdural empyema complicating sinusitis was diagnosed in two children by CAT scan. One of them presented with intracranial hypertension and paresis of one foot (syndrome of the falx cerebri). Antibiotic treatment alone without surgery (in one case with brief initial steroid therapy) brought rapid and complete clinical and radiological cure. A nonsurgical approach can now be considered in certain cases of intracranial local suppurations, given the possibility of earlier and more precise initial diagnosis and follow-up with CAT scan.

Adolescent↗
PubMed1979

[Cultures of tissue from central nervous system tumors. Part 1. Neuroglial tumors].

The authors report their experience with monolayer culture of 80 neuro-glial tumors. Of these 45 were glioblastomas and 17 astrocytomas. 7 glioblastomas gave long term lines, two of which have been transplanted into nude mice and studied by electron microscopy. The morphological and biochemical characteristics of neuro-glial tumors are discussed.

Astrocytoma↗
PubMed1978

[Anti-tumor antibodies in the blood of patients with gliomas].

Anti-tumor antibodies have been searched for with an antibody-dependent cell-mediated cytotoxicity assay in the sera of patients with glioma. Sera from 60 patients and from 25 normal individuals have been tested against cells from 8 human glioblastoma lines. 10 patients (17%) and 5 controls (20%) were found to have antibodies against one or more tumor lines. There were extensive cross-reaction between the positive sera against the different glioma cells, but the reactivity of each serum was different. The specificity of the antibodies thus detected has been investigated. The positive patients' sera were found to have a similar cytotoxic activity against unrelated tumor and normal cells. Moreover, their activity was absorbed by cells from unrelated tumors and normal platelets. These results do not support the concept of a specific humoral response of glioma patients to a possible common tumor-associated antigen.

Antibodies, Neoplasm↗