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Biomedical subjects

N Yoshimura

Publications and source records attributed to N Yoshimura.

At least 505 records · Page 28Linked to original sources
PubMed1989

[A modified technic for coronary to graft anastomosis and coronary perfusion in Bentall operation].

From September 1987 to January 1989, we performed 7 consecutive Bentall operations using a modified technique for the coronary artery to graft anastomosis with a satisfactory result. Six patients with annuloaortic ectasia and one with type 1 aortic dissection underwent the operation. After suturing a composite graft to the aortic annulus, side-holes about 8 mm in diameter were made in the graft at points corresponding to the coronary ostia. Then the graft was cut longitudinally on the side of the non-coronary cusp so as to make operative procedure easier. Four buttressed mattress sutures of 4-0 polypropylene thread were placed in the aortic wall around the coronary ostia and connected to the corresponding part of the graft. These threads were tied and then used for running sutures from inside of the graft. Even in a case where the coronary ostium was close to the aortic annulus, this procedure permitted easy and secure accessibility. Additionally, retrograde continuous cold blood cardioplegia via the coronary sinus made Bentall procedure easier and safer. Postoperative angiography revealed no stenosis or deformity of the coronary artery and no leakage from the suture line. All patients are doing well in NYHA functional class I.

Adolescent↗
PubMed1989

[A case of focal nodular hyperplasia in the liver].

A case of focal nodular hyperplasia of the liver was reported, in which various diagnostic imaging modalities were performed. Hepatic colloid scan (SPECT) in this case shows typical focal accumulation in the lesion. Ultrasonic image shows a sharply demarcated hypoechoic lesion in the left lobe. But no significant informations of this tumor were obtained in the plain CT, CE-CT and angiography. Echo-guided biopsy was performed and focal cirrhotic finding in the lesion without liver cirrhosis was obtained.

Adult↗
PubMed1989

Calcium-dependent phosphorylation of proteins in rabbit ciliary processes.

Calcium-dependent phosphorylation of endogenous substrate proteins in albino rabbit ciliary processes was studied by SDS-polyacrylamide gel electrophoresis and autoradiography. In the soluble fraction, a modest augmentation of phosphorylation was observed by Ca2+ alone and together with the additional activators, calmodulin (CAM) or phorbol myristate acetate (PMA). However, there was a greater enhancement of protein phosphorylation by Ca2+ and activators in the particulate fraction. The degree of Ca2+-CAM-dependent protein phosphorylation was greater than that of Ca2+-PMA-dependent phosphorylation. Endogenous substrate proteins for Ca2+-CAM-dependent protein kinases had apparent molecular sizes of 205,170,150,130,77,58,40,32 and 18 kDa. Phosphorylation of the 58 kDa protein band was strongest. This protein was identified as vimentin on the basis of its behavior with Triton-X100 treatment, and by Western blotting using anti-vimentin antibody. Endogenous substrates of protein kinase C (Ca2+-PMA-dependent) were located at 87 kDa and possibly in the 56 and 54 kDa protein bands. A 50 kDa protein was found to be phosphorylated in the presence of Ca2+ alone, and was not affected by the presence of other activators (CAM or PMA). A Ca2+-dependent dephosphorylation of a 43 kDa protein was observed, and some proteins rapidly phosphorylated by Ca2+-CAM kinase were also relatively quickly dephosphorylated at incubation times greater than 1 min.

Animals↗
PubMed1989

Cyclic nucleotide-dependent phosphorylation of proteins in rabbit ciliary processes.

Cyclic nucleotide-dependent protein phosphorylation in albino rabbit ciliary processes was studied in particulate and soluble fractions of the tissue by the technique of SDS-polyacrylamide gel electrophoresis and autoradiography. In the presence of gamma-32P-ATP, the soluble fraction showed increased phosphorylation of proteins of 200, 32 and 16 kDa molecular weight when 10 microM cAMP was added. Protein phosphorylation increased with time up to 5 min. No significant augmentation of phosphorylation was observed in the presence of 10 microM cGMP compared to control. In the particulate fraction, proteins with molecular weights of 200, 160, 105, 72, 58, 32 and 16 kDa showed increased phosphorylation in the presence of 10 microM cAMP. Phosphorylation caused by the addition of cAMP was maximal between 30 sec and 1 min for the particulate membrane fraction, but with longer incubation times the incorporation of phosphate residues decreased. The same molecular weight proteins of the membrane fraction that were phosphorylated in a cAMP-dependent manner were phosphorylated in the absence of exogenous cAMP by addition of either the catalytic subunit of cAMP-dependent protein kinase or activators of membrane-bound adenylate cyclase such as l-isoproterenol, vasoactive intestinal peptide, aluminum fluoride or forskolin. A cAMP-dependent dephosphorylation of a 56 kDa protein was observed in the membrane fraction. Cyclic GMP did not cause observable changes in the pattern of protein phosphorylation in the particulate fraction of rabbit ciliary processes.(ABSTRACT TRUNCATED AT 250 WORDS)

Adenylyl Cyclases↗
PubMed1989

Effects of roxatidine acetate hydrochloride and cimetidine on the pharmacokinetics of theophylline in healthy subjects.

The effects of roxatidine acetate hydrochloride and cimetidine during multiple dosing on the pharmacokinetics of theophylline was studied in nine healthy volunteers, five smokers and four non-smokers, in comparison with placebo treatment. Cimetidine reduced the terminal elimination rate constant and the total body clearance of theophylline from 0.119 to 0.101 h-1 (p less than 0.05) and from 31.2 to 26.5 ml/min (p less than 0.05), respectively, in comparison with those of placebo. There was no significant change in the volume of distribution (16.3 l for placebo and 15.5 l for cimetidine) and the renal clearance (4.5 ml/min for placebo and 3.9 ml/min for cimetidine). Roxatidine acetate hydrochloride did not significantly influence theophylline disposition in comparison with placebo treatment. The interaction of cimetidine on theophylline disposition was observed in both smokers and non-smokers, but the degree was greater in smokers.

Adult↗
PubMed1989

[A case of congenital myotonic dystrophy with infantile autism].

An 11-year-old girl with congenital myotonic dystrophy and infantile autism was reported. Her mother also suffered from typical myotonic dystrophy. Since her birth, the patient had been floppy, and showed bilateral talipes equinus at 1 year of age. Her subsequent psychomotor and speech development has been retarded. She showed autistic behavior and persistence to the sameness before 2 years old. She was admitted to Sawarabien at the age of 10 years. She could not talk anything but could understand simple, oral messages. Although she had severe degree of mental retardation, her ability for matching figures was relatively well reserved. Her autism was so manifest that it could not be explained by the degree of mental retardation. Neurological examinations revealed that she had facial diplegia, inverted V-shaped mouth, high-arched palate, talipes equinus, percussion myotonia of the tongue, generalized muscular atrophy and weakness, lordosis, areflexia, and congenital cataracta. The serum CPK was slightly elevated. EMG showed a myopathic pattern but did not show any myotonic discharge yet. The brachioradial muscle was biopsied and examined by light- and electron-microscopy. It mainly showed mild varieties of muscle fiber diameter and internal nuclei. Ultrastructurally, irregularly indented central nuclei and perinuclear degeneration of myofibrils associated with secondary lysosomes, lipid droplets and glycogen granules were revealed. Ventricular dilatation and some dysfunction of the brain were also revealed by CT scan and EEG respectively. The present case suggests that congenital myotonic dystrophy can be added into the disease group associated with infantile autism.

Autistic Disorder↗
PubMed1989

Immunochemical characterization of the suppressor factor from early human decidual cells.

An immunosuppressive factor was obtained from culture supernatants of early human decidual cells. The suppressor factor was concentrated by gel filtration in a fraction with a molecular weight between 43,000 and 67,000 daltons. It was further purified by biochemical methods. Four peaks were obtained in the fraction with molecular weight between 43,000 and 67,000 daltons by anion exchange chromatography. Only the second peak had immunosuppressive activity in MLR. Lentil-lectin affinity chromatography of this suppressor factor showed that the suppressor factor had no affinity for lentil-lectin sepharose. Isoelectric focusing of the suppressor factor demonstrated four bands. The protein isoelectric (PI) point was approximately 7.50 in one band and between 6.85 and 7.35 in the other three bands. These results demonstrate that the suppressor factor is not glycoprotein but protein, whose PI is between 6.85 and 7.50. The suppressive effect of this purified factor on lymphokine production and lymphocyte activation was investigated. The addition of the purified suppressor factor to a culture of PBL stimulated with PHA suppressed not only IL-2 production and gamma-INF production, but also BSF-2 production. IL-2 receptor expression and transferrin receptor expression of PBL stimulated with PHA were also suppressed by addition of the suppressor factor. These results demonstrate that this suppressor factor inhibits lymphokine production and lymphocyte activation.

Biological Assay↗
PubMed1989

[A clinical study on cefuzonam concentrations in myocardium in open heart surgery].

The distribution of cefuzonam (CZON) was studied in 20 adults undergoing open heart surgery. In groups I (n = 11) and II (n = 9). CZON (2g) was intravenously infused at the time of induction of anesthesia; and in group II an additional 2 g was administered at the time of commencement of extracorporeal circulation (ECC). Just prior to and following the ECC, samples of blood and right auricle were taken for examination. CZON concentrations in serum and myocardium were measured using the thin-layer cup method with Esherichia coli NIHJ as the test organism. CZON concentrations in myocardium prior to ECC were 48.9 micrograms/g and 39.0 micrograms/g for groups I and II, respectively. The ratios of intramyocardial to serum CZON concentration were 0.40 and 0.45 for groups I and II, respectively, revealing no significant difference between the 2 groups. Following ECC, intramyocardial CZON concentrations were 20.8 micrograms/g for group I and 33.2 micrograms/g for group II; while the ratios were 0.43 and 0.59 for groups I and II, respectively. Again, there were no significant differences. From the above findings it may be concluded that: 1. There was good distribution of CZON in myocardium, with a concentration well above MIC80. 2. Therapeutic concentrations of CZON were maintained in myocardium for 6 hours, suggesting that initial infusion of 2 g CZON is sufficient for prophylaxis in routine open heart surgery.

Cardiac Surgical Procedures↗