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Biomedical subjects

N Weir

Publications and source records attributed to N Weir.

30 records · Page 2Linked to original sources
PubMed1993

Expression, purification and characterization of B72.3 Fv fragments.

The Fv fragment of the antibody B72.3 has been produced by expression in both a mammalian and microbial system, namely Chinese hamster ovary (CHO) cells and Escherichia coli. In both cases secretion of the Fv into the culture medium was achieved, with equivalent amounts of Vh and Vl produced. The yield of Fv from CHO cells was 4 mg/l in roller-bottle culture. E. coli proved to be a more productive system with yields of 40 mg/l in shake flasks rising to 450 mg/l in fermentations. B72.3 Fv from both sources was capable of binding to antigen with similar binding ability to the Fab' fragment. A detailed sedimentation analysis, both by velocity and equilibrium techniques, revealed that the two domains of Fv are associated at high concentrations at pH values close to neutral, but dissociate at concentrations lower than approx. 0.5 mg/ml. Individual Vh or Vl polypeptides are not able to bind to the antigen and thus these results suggest that the antigen promotes assembly of Fv at the low concentrations used in the antigen-binding assays. At a pH value of 1.9, Vh and Vl are completely dissociated even at very high concentrations and are apparently unfolded at low solute concentrations. Small-angle X-ray scattering was used to measure a radius of gyration of 1.75 +/- 0.2 nm (17.5 +/- 2 A) for Fv.

Animals↗
PubMed1993

Hearing impairment and ear pathology in Nepal.

A stratified random cluster sample of 15,845 subjects was performed in two regions of Nepal to determine the prevalence and main causes of hearing impairment (the most common disability) and the prevalence of ear disease. Subjects reporting current ear pain, or ear discharge, or hearing impairment on direct questioning by a Nepali health worker (primary screening failed), had otoscopy and audiometry (using the Liverpool Field Audiometer) performed, and a questionnaire administered relating to past history. In every fifth house subjects who passed the primary screening (1,716 subjects) were examined to assess the false negative rate of screening. An estimated 16.6 per cent of the study population have hearing impairment (either ear worse than 30 dB hearing threshold level (HTL) 1.0-4.0 kHz, or 50 dB HTL 0.5 kHz), and 7.4 per cent ear drum pathology, equivalent to respectively 2.71 and 1.48 million people extrapolated to the whole of Nepal. Most hearing impairment in the school age group (55.2 per cent) is associated with otitis media or its sequelae. Probably at least 14 per cent of sensorineural deafness is preventable (7 per cent infectious disease, 3.9 per cent trauma, 0.8 per cent noise exposure, 1 per cent cretinism, and 1 per cent abnormal pregnancy or labour). Most individuals reporting current ear pathology (61 per cent) had never attended a health post, and of those receiving ear drop treatment, 84 per cent still had serious pathology. Of subjects who reported ear drop treatment at any time, 31 per cent still had serious pathology. The use of traditional remedies was prevalent.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1991

Ear surgery camps in Nepal and the work of the Britain Nepal Otology Service (BRINOS).

The Britain Nepal Otology Service (BRINOS), founded as a registered charity in 1988, has so far held four ear surgery camps in Nepal, sponsored and organised a nationwide survey of the prevalence and causes of ear disease and deafness and is sponsoring a pilot scheme of primary care for ear disease. Seven thousand outpatients have been seen, 270 major and 170 minor ear operations have been performed in the four ear surgery camps.

Cholesteatoma↗
PubMed1986

Prolonged endotracheal intubation in neonates with hyaline membrane disease.

It is well documented that prolonged endotracheal intubation can lead to subglottic trauma and stenosis. Newborn infants with hyaline membrane disease often require assisted ventilation for prolonged periods of time. We examined 11 such children ranging in age from four months to 4.25 years by endoscopy. Some abnormality was found in every child, and in nine there was minor narrowing of the airway.

Airway Obstruction↗
PubMedDate not supplied

Frailty: a public health perspective.

Traditionally, frailty has been conceptualized as reflecting an inevitable decline in abilities usually associated with physical aspects of aging. More recent thinking now sees frailty as reflecting an interaction among individual factors and a range of environmental elements. These emerging models, however, continue to consider frailty as a condition that resides within the individual rather than a situation that exists for the individual. We present a new model that defines frailty as occurring when there is diminished ability to carry out the important practical and social activities of daily living. Factors related to the occurrence of frailty are considered, as are the theoretical and practice implications of viewing frailty as a social construction.

Activities of Daily Living↗
PubMedDate not supplied

The quality of life of seniors living in the community: a conceptualization with implications for public health practice.

Maintaining and improving the quality of life (QOL) of seniors in the community is an increasingly important goal of public health planning and programming. To provide authentic assessments of seniors' quality of life, a partnership of public health workers and university researchers, working closely with seniors in the community, developed the Quality of Life Profile: Seniors Version (QOLPSV). Administration of the QOLPSV to a wide range of North York, Ontario seniors (n = 205) participating in community-based healthful living programs provided evidence of the instrument's psychometric integrity. Additionally, results indicated specific areas of need among seniors, suggesting shifts in emphasis in program delivery. Additional public health applications of a quality of life perspective are outlined.

Aged↗
PubMedDate not supplied

Canadian approaches to the promotion of health in small workplaces.

The promotion of health in small workplaces is particularly challenging. Research was conducted to identify the types of agencies offering prevention and promotion services to small workplaces, and to describe the activities and strategies used to engage this "hard-to-reach" population. This paper reports on a survey of 58 agencies located across Canada. Agencies were mostly of recent origin, many having arisen in response to legislative and other government initiatives, and of two essential types: those that used the workplace as a site for addressing general lifestyle health behaviours, and those addressing the occupational determinants of health. Their location, staffing and program focus reflected their orientation. Factors believed by respondents to influence program effectiveness are summarized. The paper notes the top-down origins of many prevention/promotion efforts, their inherent management bias, and the differing perspectives that underlie the lifestyle and occupational approaches to workplace health. A call for integration of the two approaches is made.

Canada↗