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Biomedical subjects

N V Rodionova

Publications and source records attributed to N V Rodionova.

28 records · Page 2Linked to original sources
PubMedDate not supplied

[Glycosaminoglycan metabolic disorders in periosteal and endosteal osteogenic cells in phenol poisoning].

The analysis of 35S sulphate incorporation into the growing bone structures in the experiment with 2-3 week guinea pigs subjected to long-term phenol intoxication made it possible to conclude that 35S sulphate assimilation by the osteogenic cells of periost and endost is less intensive in the treated animals, particularly in those receiving a large dose of phenol, (40 mg/kg) than in the control ones. This testifies to the reduction of the biosynthesis rates of sulphated glycosaminoglycans in the bone growth regions.

Animals↗
PubMedDate not supplied

[Structural identity of the multinuclear chondro- and osteoclasts in developing bone].

Distributional peculiarities and the ultrastructure of multinuclear elastic cells have been studied in the developed thighbone of rat and rabbit fetuses and new-borns. Multinuclear chondro- and osteoclasts exhibit no noticeable structural specificity with respect to the resorption substrate and are considered to be one and the same type of clastic cells whose main function is to resorb the mineralized tissue.

Animals↗
PubMedDate not supplied

[Ultrastructure of DNA-synthesizing cells in an enchondral focus and osteogenic precursor cells].

It was shown by light and electron 3H-thymidine autoradiography that in the zones of endochondral osteogenesis in a tubular bone the little-differentiated perivascular cells are characterized by the most intensive proliferation among the DNA-synthesizing cells of the stroma. A comparison of the ultrastructural features and the distribution of labelled cells, their topographic interrelations at different times following the isotope injection suggests that during histogenesis in the endochondral centres the little-differentiated perivascular cells become spatially separated and are involved in the formation of the stromal cellular elements (in particular, fibroblasts, reticular cells, osteoblasts). In the developing and mature stroma, as well as in the endost, the little-differentiated forms of the population are preserved; the number of DNA-synthesizing cells among them decreases with age. It is suggested that the population includes stromal stem cells.

Aging↗
PubMedDate not supplied

Features of the reproduction of epiphyseal cartilage perivascular cells according to the results of an autoradiographic study.

In the system of a vascular-cellular complex of cartilage epiphyses the perivascular cells exhibited the highest proliferative activity, as revealed by autoradiography with labelled DNA precursor (3H-thymidine). The constant loss of cells after division in this population is probably associated with their differentiation and transition into another state. This phenomenon is strongly pronounced at the centre of enhondral ossification, and perivascular cells could be considered as one of possible sources of histogenesis developed in cartilage epiphyses.

Animals↗
PubMedDate not supplied

[Ultrastructure of the ductal epithelium in the human pancreas in chronic pancreatitis].

Three different kinds of cells are distinguished within the epithelium of the pancreas ducts in patients with chronic pancreatitis: main ductal cells among which there are "light" and "dark" forms differing from each other in the level of their biosynthetic apparatus development and being in the state of different functional activity; endocrine cells with morphological features of chromaffin-like and D-cells of the gastrointestinal tact; migrating cells (histocytes, granulocytes, lymphocytes). Characteristic features of destruction in the pathologically altered epithelium are described.

Chronic Disease↗
PubMedDate not supplied

[Reproduction of cells of the osteogenic layer of periosteum].

The H3-thymidine autoradiography method was used to prove that the population of osteoblasts in the periosteum of growing bone is replenished due to the reproduction and differentiation of perivascular cells and preosteoblasts. The differentiation of these cells into osteoblasts is accompanied by reduction of their proliferative activity due to an increase in the duration of generation time and progressive getting out of cells from the mitotic cycle.

Animals↗
PubMedDate not supplied

[The role of osteoclasts in maintaining calcium homeostasis in newts].

The intensity of osteoclastic resorption and calcium contact were investigated in intact limb bones of the newts flown onboard of biosatellite Cosmos-2229 after amputation of their forelimbs and tail. X-ray microanalysis has shown an increase of calcium content in the bones on the 20th day after operation. Histological study revealed activated osteoclastic resorption on the inner surface of long bones. The newts exposed to weightlessness after the operation had the same level of bone mineralization as the operated ground control ones, but the number of polynuclear osteoclasts increased to a lesser extent.

Animals↗
PubMedDate not supplied

[The effect of microgravity on osteogenic cells in culture. Ultrastructural research].

In order to estimate the influence of microgravity on osteogenic cells a comparative ultrastructural study of preosteoblastic cells of line MN7, cultivated in space and in ground conditions has been carried out. Morphometric analysis has revealed that in cells exposed during 9 days on board the biosatellite Cosmos 2229 endoplasmic reticulum was not so expensive and the number of myeline-like structures increased as compared to the ground control. Percentage of cells with nucleoli is lower in the flight samples as well. The increase in specific volume of lipid droplets and changed structure of mitochondria testifies to deviation in energy metabolism. The reaction of osteogenic cells to microgravity resulting in suppression of specific functions may contribute to alterations in the bone metabolism under the space flight conditions.

Animals↗