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Biomedical subjects

N Utsumi

Publications and source records attributed to N Utsumi.

At least 37 records · Page 2Linked to original sources
PubMed1990

[Epidermoid cyst of the oral floor with massive deposition of melanin in the cystic epithelium].

Dermoid cyst or epidermoid cyst of the oral cavity usually occurs in the floor of the mouth. We encountered a 21-year-old woman who had developed epidermoid cyst in the oral floor. Since infancy she had been aware of a swelling in the oral floor. Recently, the swelling began to grow larger gradually. The lesion was excised under general anesthesia by intraoral approach. The swelling was spherical in shape, measured 50 x 45 x 40 mm in size, and weighed 40 g. Histopathological examination revealed keratinized, stratified squamous epithelium on the cyst wall and marked deposition of melanin at the base of the epithelium.

Adult↗
PubMed1989

[Comparative WGA-lectin histochemical study on the mandibular condylar cartilage from normal and low phosphate-vitamin D deficient rats].

Mandibular condylar cartilage from normal and low phosphate-vitamin D deficient rats were investigated by lectin histochemistry utilizing wheat germ agglutinin (WGA). We obtained the following results: 1) The cartilage from low phosphate-vitamin D deficient animals consisted predominantly of hypertrophic chondrocytes with abundant extracellular matrix. 2) The territorial matrix of the hypertrophic cell zone of normal cartilage was intensely labelled with WGA, whereas the binding affinity of this region in low phosphate-vitamin D deficient animals was significantly diminished. 3) Compared to normal cartilage, WGA binding sites were demonstrated in the inter-territorial matrix of low phosphate-vitamin D deficient animals to a moderate degree. 4) Abolishment of the territorial WGA binding affinity in low phosphate-vitamin D deficient animals was presumed to indicate the accumulation of chondroitin sulfate which is believed to be a strong chelator of calcium ion.

Animals↗
PubMed1988

Calcifying odontogenic cyst immunohistochemical detection of keratin and involucrin in cyst wall.

Calcifying odontogenic cysts (COC) were immunohistochemically described using different keratin proteins and involucrin as well as histopathology. The cystic lining epithelium was composed of calcifying, keratinizing, squamous, and columnar epithelial cells, and included calcified masses of irregular shape and various size as well as ghost cells. Calcifying epithelium gave negative or only trace staining for keratins detected with low molecular keratin (PKK1), but were regularly positive with high molecular keratin (KL1) and polyclonal antibody for keratin (TK). They were occasionally positive for involucrin. The cells located in the periphery of the calcified masses had a particular abundance of high molecular weight and total keratins (KL1 and TK). Calcified bodies and ghost cells were devoid of any immunoreactivity. Squamous epithelium was relatively similar to that of normal squamous cell epithelium in the oral mucosa. It were most commonly found in columnar cystic epithelial cells which displayed intense staining with all immunoreagents. It is postulated that such epithelial cells may have a strong potentiality to transform into ghost cells or to undergo metaplasia. They may develop altered synthesis of homogenous acellular materials and finally become transformed into calcifying epithelium containing dystrophic calcified masses.

Adolescent↗
PubMed1986

Binding of wheat germ agglutinin in the matrix of rat tracheal cartilage.

The binding of wheat germ agglutinin (WGA) to the extracellular matrix of rat tracheal cartilage was studied at both the light and electron microscopic levels. The detection of binding sites was accomplished by a postembedment method using the direct fluorescence technique for light microscopy and the avidin-biotin bridging system for electron microscopy. Distinct fluorescence was observed in the pericellular region of chondrocytes, and this fluorescence was completely removed after treatment with 4 M guanidine hydrochloride. By electron microscopy, the reaction products as found in the pericellular region were not observed in the interterritorial collagenous matrix, confirming a similar distribution as found by fluorescence microscopy. These results show that WGA-binding sites are present in pericellular matrical substances, which are known to be rich in proteoglycan and glycosaminoglycan complexes and which exhibit similar staining with antibodies to proteoglycans or with cationic dyes. As WGA binds to N-acetyl-glucosamine and N-acetyl-neuraminic acid residues, the pericellular matrix of rat tracheal cartilage appears to consist of proteoglycan having a high concentration of these saccharides.

Animals↗
PubMed1986

Effect of calcium deficiency on the content of nonreducible crosslinks in rat mandibular bone.

The effect of calcium deficiency on the content of the nonreducible cross-linking amino acid, pyridinoline, in rat mandibular bone was studied. An apparent decrease in serum and bone calcium levels was observed beginning at four weeks after initiation of a calcium-deficient diet. The pyridinoline content in the bone of calcium-deficient animals began to increase at four weeks and thereafter increased markedly as compared to that of controls. Collagen content increased significantly six weeks after calcium deficiency. These results suggest that calcium deficiency inhibits mineralization and promotes the formation of pyridinoline in rat mandibular bone.

Amino Acids↗
PubMed1986

Immunoperoxidase investigation of Regan isoenzyme of alkaline phosphatase in maxillary sinus carcinomas.

The Regan isoenzyme of alkaline phosphatase is known to be produced ectopically by various malignant tumors. To assess whether this isoenzyme is expressed in maxillary sinus carcinomas, immunostaining for Regan isoenzyme was carried out on 35 maxillary sinus carcinomas comprising 27 squamous cell carcinomas, 6 adenocarcinomas, and 2 adenoid cystic carcinomas. The enzyme was shown to be present in 11 of 27 cases of squamous cell carcinoma, 2 of 6 cases of adenocarcinoma, and 1 of 2 cases of adenoid cystic carcinoma. Regan isoenzyme was identified in the cytoplasm and/or cell membrane of squamous cell carcinoma cells, whereas it was demonstrated mainly on the luminal membrane and in the secretions of both adenocarcinoma and adenoid cystic carcinoma cells. These findings show that Regan isoenzyme does, indeed, occur in maxillary sinus carcinomas.

Adenocarcinoma↗
PubMed1986

The dentinogenic ghost cell tumor.

An unusual case and they of dentinogenic ghost cell tumor, a peculiar variant of calcifying odontogenic cyst, is reported. The tumor consisted predominantly of ghost cells with occasional areas of "dentinoid" in close proximity to masses of ghost cells. The presence of ameloblastoma-like nests of epithelium was characteristic of the present case and they were interpreted as a degenerative phenomenon exhibiting an inductive effect with formation of abundant ghost cells, occurring within a pre-existing ameloblastoma. The term of "dentinogenic ghost cell tumor" is employed for this unique lesion presented in this report. The literature describing the neoplastic potential of the calcifying odontogenic cyst is briefly reviewed.

Adult↗
PubMed1986

Comparison between the effects of dexamethasone and indomethacin on bone wound healing.

The area of wound holes made in the parietal bone of 4-week-old rats was measured at 1, 2 and 4 weeks after the operation using Alizarin red S staining without the use of histological sections. Dexamethasone and indomethacin were administered s.c. after the operation every day except Sunday. The inhibitory action of dexamethasone on bone wound healing was stronger than that of indomethacin, as was its inhibitory action on skin wound healing. Inhibition of the age-related decrease in radiolucency of femurs and some changes in serum calcium level were also observed by dexamethasone administration, but not by indomethacin. These results suggest that dexamethasone inhibits bone wound healing through its effects on calcium metabolism in addition to its general inhibitory action on wound healing which is shared with indomethacin. Dexamethasone also strongly inhibited the decrease in Alcian blue stainability which occurs with an increase in age, both in the normal portion and in the wound hole portion of the calvaria, whereas indomethacin showed a weak inhibitory effect only in the wound hole portion. This result suggests that one of the mechanisms by which dexamethasone inhibits bone wound healing may be based on the retardation of mineralization caused by the inhibition of removal of acid mucopolysaccharide.

Animals↗
PubMed1985

Reduced collagen biosynthesis in oral mucosa of beige (Chediak-Higashi) mice.

Oral mucosa of beige (Chediak-Higashi) mice had decreased levels of collagen synthesis and prolyl hydroxylase activity compared with normal animals. No significant difference was observed in non-collagen protein synthesis between the two groups. These results suggest that decreased collagen biosynthesis in oral tissues may be partially involved in the increased incidence of periodontal disease in the Chediak-Higashi syndrome.

Animals↗