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Biomedical subjects

N Uehara

Publications and source records attributed to N Uehara.

At least 91 records · Page 5Linked to original sources
PubMed1980

Elevated contents of spermidine and spermine in the erythrocytes of cancer patients.

Red blood cells (RBC) from 69 patients with advanced cancer and 37 healthy controls were subjected to polyamine determination by using high-performance liquid chromatography. The polyamine contents in normal human RBC were spermidine 15.04 +/- 3.63 nmol and spermine 8.82 +/- 3.12 nmol per 10(10) RBC. Spermidine and spermine levels in RBC were elevated in patients with cancer (p less than 0.005). Serial studies in seven patients with cancer indicated that both polyamines in RBC were reduced after successful surgery. Our data indicate that the determination of polyamine levels in RBC is clinically useful as a marker of disease activity in patients with cancer.

Chromatography, High Pressure Liquid↗
PubMed1980

Myelofibrosis with skin lesions.

An elderly male patient with primary myelofibrosis developed many papules on the trunk and later numerous subcutaneous nodules on the face. Histologically these lesions showed the features of extra-medullary haematopoiesis consisting of all the three components, i.e. myeloid, erythroid and megakaryocytic elements, findings never reported in the skin.

Aged↗
PubMed1980

Elevated polyamine content in erythrocytes of malignant lymphoma patients.

The polyamine content in erythrocytes was determined in an attempt to establish a biological marker of tumor growth in patients with malignant lymphoma. Erythrocytes from 24 patients with malignant lymphoma and 40 healthy controls were subjected to polyamine determination, using high-performance liquid chromatography. The spermidine and spermine levels in normal human erythrocytes were 15.06 +/- 3.61 and 8.79 +/- 3.12 nmol/10(10) cells, respectively. Spermidine and spermine levels in erythrocytes of lymphoma patients were 14.16 +/- 4.99 and 13.06 +/- 5.37 nmol/10(10) cells in the stage I and II groups, 21.25 +/- 3.80 and 14.17 +/- 6.11 in the stage III groups, and 34.17 +/- 10.37 and 24.39 +/- 16.08 in the stage IV group, respectively. The levels of both polyamines were significantly elevated (P < 0.005) in erythrocytes of stage IV patients, while those of stage I and II patients were within the normal range. Serial studies in 8 patients indicated that the levels of both polyamines in erythrocytes correlated well with the clinincal state. Thus, the determination of polyamines in erythrocytes should be clinically useful to determine the state of progression of malignant lymphoma.

Adolescent↗
PubMed1978

Sensitive fluorimetric method for the determination of putrescine, spermidine and spermine by high-performance liquid chromatography and its application to human blood.

A fast and sensitive method for the determination of putrescine, spermidine and spermine by high-performance liquid chromatography is described. These compounds are converted to their fluorescent dansyl derivatives and are separated by a reversed-phase chromatographic system (Micropak CH-10) with water and acetonitrile as mobile phase. The sensitivity of the method is 30 pmoles. The application of the method to the determination of polyamines in blood is described. It was found that most of the polyamines circulating in blood are localized in the erythrocytes, their content in normal human blood being spermidine 14.1 +/- 3.1, and spermine 8.4 +/- 2.8 nmoles/ml packed erythrocytes. The polyamine level in serum is less than 0.1 nmole/ml. The polyamine content of the erythrocytes from patients with malignant neoplasms was significantly elevated.

Chromatography, High Pressure Liquid↗