Histological, histochemical and electron microscopic study on alcoholic liver disease.
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Acute Disease↗
Biomedical subjects
N Tudose
Publications and source records attributed to N Tudose.
Significance of the expression of hormone receptors and of p53 protein in breast carcinomas.
A certain proportion of breast cancers contain hormone receptors--specific proteins with high affinity and capacity of selective tie of these hormones. There were selected 30 patients presenting breast cancer, treated at the surgical departments from the County Hospital of Timişoara between 1993 and 1995. The identification of hormone receptors was done on 10% formalin-fixed and paraffin-embedded tissue specimens. As anti-hormone receptors, monoclonal and polyclonal antibodies were used. The immunohistochemical identification of positive to hormone receptors cancer cells was obtained, by nucleus staining. Immunohistochemical assay of the accumulation of p53 protein seems to be an efficient means of selecting breast neoplasms. p53 immunostaining is positive at the level of malignant cells' nuclei and negative for normal cells. From the 30 breast carcinomas, 19 (63.3%) were ER+ and 15 (49.9%) were PR+, resulting an increase of the frequency of ER+ tumors together with the age of the patients; 9 cases (30%) expressed p53 protein, harmonizing with the data in the literature.
Adult↗
Expression and significance of tumoral suppressor p53 gene in lung adenocarcinomas.
The mutations of p53 tumoral suppressor gene are the most frequent genetic modifications identified till now in lung cancer, suggesting that these alterations represent critical stages in malignant cellular transformation of respiration ways. Conformational changes induced by these mutations are associated with stabilisation of the product of p53 gene and the accumulation of the mutant protein in malignant cells' nuclei, in quantities that can be immunohistochemically detected: immunohistochemistry can be used as an indirect indicator of the genome alteration. Eight cases of lung adenocarcinoma were immunohistochemically analysed (formalin-fixed and paraffin-embedded specimens), in order to find the p53 suppressor expression. The overexpression of p53 protein was detected using an antigenous system and Monoclonal Mouse Anti-Human p53 protein, and it was detected in 5 (62.5%) of the 8 examined adenocarcinomas. In the examined cases, p53 overexpression was limited only to neoplastic cells, the nuclear staining being considered specific. p53 high level was correlated to: histological differentiation degree, smoking, the stage of the tumor and to the survival rate of the patients. The association of smoking with p53 overexpression suggests that p53 gene is a target of specific mutagenes in smokers.
Adenocarcinoma↗
Prognostic significance of neuroendocrine differentiation in carcinoma of the prostate.
Biopsies taken by core-needle biopsy and/or transurethral resection to 68 patients with prostatic neoplasia stage III and IV, were studied morphohistochemically. Morphological observations showed high incidence of mixed histopathological forms. The Churukian-Schenk and Garvey silver impregnations were used in order to identify associated neuroendocrine differentiations. Three cases presented monomorphic small cell proliferation which contained numerous argyrophilic cytoplasmic granulations. The prognosis in these cases was poor--the patients died because of neoplastic extension within 5 to 9 months following diagnosis. Neuroendocrine cells were identified in 15 cases, 6 of which presented a malignant pattern. Taking into consideration the hormonal resistance of malignant cells with argyrophilic granulations, the necessity of chemotherapy associated to hormonotherapy is discussed.
Cell Differentiation↗
Carcinoid tumor with uterine location. Case report.
A carcinoid is defined as a tumor arising from endocrine cells with neurosecretory characteristics belonging to the APUD system. These cells are most frequently observed in the digestive tract and lungs. Uterine location is rare. This paper presents the case of a 21-year old patient with uterine carcinoid tumor. In order to establish the histopathologic diagnosis of the carcinoid tumor, we used optical microscopy examination in haematoxylin-eosin, argentic impregnation in Fontana-Masson staining and an immunohistochemical reaction using monoclonal antibody to the S100 protein. Due to the intracytoplasmatic granulations shown in Fontana-Masson staining and in the immunohistochemical reaction to S100 protein which confirm the neurosecretory character of the tumoral cells, we included this tumor in the group of tumors with neuroendocrine differentiation.
APUD Cells↗
Intestinal metaplasia and dysplastic modifications in H. pylori gastritis.
Certain studies regarding modifications of gastric mucosa in the presence of infections produced by H. pylori add a new element in the pathology of gastric lesions with a high malignancy potential. From the certain premalignant lesions, a special place is occupied by intestinal metaplasia and by gastric dysplasia, because of their high frequency. This study is based upon the interpretation of 130 gastric biopsies in which the identification of H. pylori was pursued (by HE staining, Giemsa), o intestinal metaplasia and their types (by PAS-Blue Alcian ph 2.5 staining and High Iron Diamine-Blue Alcian ph 2.5) and the presence of neoplastic lesions. Particular aspects of our study are given by the association of H. pylori infection with certain types of intestinal metaplasia, with moderate or severe dysplastic lesions and with gastric carcinomas (especially the "intestinal" type).
Gastritis↗
Researches on viral hepatitis markers correlated with pathomorphological and clinical aspects in chronic hepatitis.
A number of 112 patients hospitalized for chronic hepatitis and liver cirrhosis were investigated. According to the results of the pathomorphological examinations of liver biopsies, 29 (25.8%) were cases of persistent chronic hepatitis (PCH), 39 (34.8%) cases of active chronic hepatitis (ACH), and 44 (39.2%) cases of liver cirrhosis. The prevalence of the female sex was observed in PCH (61.6%) and ACH (64.7%) cases, whereas in liver cirrhosis the sex distribution was more balanced (53.3% patients were males). The patients' mean age ranged from 34 to 38 years in PCH cases, from 44 to 46 years in ACH cases and from 50 to 57 years in liver cirrhosis. The postviral cirrhosis was three times more frequent in the female sex (77%), while the alcoholic and mixed cirrhoses (of an associated alcoholic and viral etiology) were prevalent in males (63.2%, respectively 72.2%). Serological tests for detection of the serological markers of hepatitis viruses B (HBV), C (HCV) and Delta (HDV) were performed for the purpose of studying the correlations between the pathomorphological findings and the viral markers. Among the 39 patients with ACH, HBV markers were detected in 13 cases (33.3%), anti-HCV antibodies in 10 (25.6%), and associated HBV-HCV, respectively HBV-HDV infections in 9 (23%) cases (6, respectively 3 patients). In the remaining 7 cases (17.9%) of ACH, there were no serological markers in the three hepatitis viruses. Of the 29 patients with PCH, 17 cases (58.6%) displayed HBV markers, 4 (13.7%) anti-HCV antibodies, in one patient (3.4%) an associated HBV-HCV infection was present, and in 7 patients (24.1%) markers of none of the three hepatitis viruses could be identified. Of the 44 patients with liver cirrhosis, HBV markers were detected in 17 cases (38.6%), anti-HCV antibodies in 9 (20.4%) and associated HBV-HCV and, respectively, HBV-HDV infections in 11 cases (25%) (9, respectively 2 cases). In 7 (15.9%) of the 44 patients with cirrhosis, markers of none of the three hepatitis viruses could be identified.
Adult↗
Immunohistochemical markers in the morphological diagnosis of lung carcinoma.
Conventional histopathological criteria based on light microscopy are used in pulmonary oncologic pathology in order to establish the diagnosis of tumor, but most frequently they are insufficient, accurate diagnosis requiring ultrastructural and immunohistochemical investigations. The method of immunostaining allowed some molecular marker to be evaluated. Some of them seem to be important in carcinogenesis as a general process, while others have high specificity for lung tumors. Estimation of EGFR and c-erbB-2 protein immunoreactivity showed a significantly stronger staining with NSCLC and was correlated to the poor differentiation of the tumors, undergoing an aggressive biological behavior and an unfavorable prognosis. The expression of p53 protein was found in 19 cases by immunostaining with DO-7 antibody. Immunotracing of more than 50% of the tumoral cells was a predictive factor for the progression of the disease. The growing rate of tumoral proliferative activity was evaluated by immunotracing technique (MIB-1), allowing the Ki-67 index of labeling to be calculated.
Biomarkers, Tumor↗
Histologic criteria for the diagnosis of gastric low-grade malt lymphoma.
Low-grade B cell lymphoma of the stomach have the features of mucosa-associated lymphoid tissue (MALT) and is characterised by lymphoid hyperplasia and infiltrations of lymphocytes into glandular epithelium or lymphoepithelial lesions. It may be difficult to distinguish low-grade lymphomas from benign inflammatory lymphoid infiltrates. Nine primary gastric lymphomas and 20 benign lymphoid hyperplasia were investigated for the type of tumour cells, lymphoepithelial lesions. Dutcher bodies, cytologic atypia, the density of lymphoid infiltrates, invasion of the muscularis mucosae, germinal centers, reactive epithelial atypia and acute inflammation. Our results suggest that B cells proliferation, lymphoepithelial lesions, cytologic atypia and Dutcher bodies are very important for the diagnosis of low-grade MALT lymphoma. None of these was seen in inflammatory infiltrates. The presence of germinal centers, acute inflammation and reactive epithelial atypia does not exclude a diagnosis of low-grade gastric lymphoma.
Adult↗