[Multiple autoanalyzer Mark-X].
Explore the source record for details and available documents.
Autoanalysis↗
Biomedical subjects
N Tanabe
Publications and source records attributed to N Tanabe.
Autoantibodies against oxidized low-density lipoprotein (LDL) and carotid atherosclerosis in patients with rheumatoid arthritis.
OBJECTIVES: To examine the relationship between autoantibodies against oxidized low-density lipoprotein (oxLDL-Abs) and the progression of carotid atherosclerosis in patients with rheumatoid arthritis (RA). METHODS: Fifty RA patients without evidence of risk factors for atherosclerosis (RA group) and 30 healthy volunteers (normal group) were investigated. The mean intima-media thickness of the common carotid artery (mean CCA-IMT) was measured by high-resolution B-mode ultrasonography. The titer of IgG oxLDL-Abs was measured by enzyme-linked immunosorbent assay. The relationships among mean CCA-IMT, IgG oxLDL-Ab titer and patient factors such as body mass index, systolic blood pressure, diastolic blood pressure, C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and serum lipid levels were examined. RESULTS: Mean CCA-IMT, CRP, ESR and titer of IgG oxLDL-Abs were significantly higher in the RA group than in the normal group. Although mean CCA-IMT showed a positive correlation only with age in multivariate analysis, IgG oxLDL-Ab titers in the RA group were positively associated with mean CCA-IMT and independently with age and sex by multiple regression analysis. CONCLUSIONS: IgG oxLDL-Abs appear to be associated with the degree of carotid atherosclerosis in patients with RA, and are independent of traditional risk factors for atherosclerotic diseases. These results suggest a possible link between autoimmune mechanisms and accelerated atherosclerosis in RA.
Arthritis, Rheumatoid↗
The effect of low affinity platelet factor 4 (LAPF4) secreted by human megakaryoblastic cell line (MEG-01) upon human bone marrow fibroblasts.
BACKGROUND: The role of low affinity platelet factor 4 (LAPF4) in the hemopoietic microenvironment has not yet been clarified. METHODS: Low affinity platelet factor 4 (LAPF4) was purified from normal human platelets and the culture medium of the human megakaryoblastic cell line (MEG-01), and their effects upon the growth of human bone marrow fibroblasts were assessed in order to investigate the biological role of LAPF4. The purified LAPF4 was added to the culture media of human bone marrow fibroblasts up to the concentration of 200 ng/m1, and the growth rate of fibroblasts and the uptake of 3H-thymidine into fibroblasts were measured. RESULTS: The molecular weight of LAPF4 from MEG-01 was approximately 8,800, which corresponded to the monomer type of LAPF4 from normal platelets. The density of fibroblasts after 10 days of culture was 4.6 +/- 0.9 x 10(5)/ml, 7.8 +/- 0.8 x 10(5)/ml and 11.3 +/- 0.6 x 10(5) in control medium, in the medium with LAPF4 from platelets and in the medium with LAPF4 from MEG-01, respectively, which indicates that LAPF4 from MEG-01 enhanced the growth rate of bone marrow fibroblast almost 2.5 times. The uptake of 3H-thymidine into fibroblasts was significantly increased by 1,000 ng/ml of LAPF4 from MEG-01. CONCLUSIONS: These results suggest that LAPF4 may play a role in the proliferation of fibroblasts.
Adult↗