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Biomedical subjects

N Solomon

Publications and source records attributed to N Solomon.

78 records · Page 5Linked to original sources

Comparison of adrenergic receptor binding in blood cells from alcoholics and controls.

Ethanol inhibited in vitro alpha-2 adrenergic receptor binding in platelets with an IC50 of 380 mM and beta adrenergic receptor binding in lymphocytes and polymorphonuclear leucocytes with IC50 values of about 1 M. The characteristics of alpha-2 receptor binding were similar in platelets from chronic alcoholics and from non-alcoholics. The densities of beta adrenergic receptors were similar in blood cells from alcoholics and controls, although there were some changes in receptor affinities. Thus, chronic alcohol consumption does not appear to have a substantial effect on adrenergic receptor binding in blood cells.

Adult↗

Cessation of smoking.

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Behavior Therapy↗

High-throughput DNA sequencing on a capillary array electrophoresis system.

A capillary array electrophoresis apparatus capable of running and analyzing 48 DNA sequencing samples simultaneously has been constructed. The instrument uses a replaceable sieving buffer and incorporates a convenient method for introducing the buffer into the capillaries. Data from laser-induced fluorescence are collected as four separate images, one for each optical channel. The integrated data analysis software employs an open architecture that allows use of any DNA base-calling algorithm. DNA sequencing runs are completed in approx. 1 hr (approximately 500 bases), and instrument turnaround time between runs is less than 15 min. Overall, the instrument throughput is on the order of 720 templates/day, or 360,000 bases/day.

Animals↗