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Biomedical subjects

N Shimada

Publications and source records attributed to N Shimada.

At least 361 records · Page 20Linked to original sources
PubMed1981

[The phenol turbidity test for measurement of pulmonary surfactants in amniotic fluid--rapid test for fetal lung maturity (author's transl)].

A simple and sensitive procedure for the quantitative estimation of pulmonary surfactants in the amniotic fluid is described. The method is based on the formation of turbidity from the amniotic fluid surfactants with phenol. Amniotic fluid drawn through an intrauterine catheter was centrifuged at 2000 rpm for 5 min. One ml of 5% aqueous phenol solution was added to 1.0 ml of the diluted supernatant. Control solution was prepared by addition of 1.0 ml of distilled water to the supernatant, instead of phenol solution. The turbidity was measured spectrophotometrically against control at 340 nm 5 to 10 min after agitation on a Vortex mixer for 5 sec. The turbidity obtained from amniotic fluid with phenol was proportional to the increase in total phospholipids in the fluid measured enzymatically. Moreover, only lecithin in the phospholipids was related to the turbidity formation. Sphyngomyelin, lysolecithin, phosphatidylethanolamin did not produce any turbidity with phenol. The spectrophotometric reading of 87 cases (31-41 weeks) was ranged at 0.18-3.52. Four cases with lower value (0.40) showed neonatal respiratory problem. The phenol turbidity test is more useful for the detection of fetal lung maturity comparing with the generally used shake test.

Amniotic Fluid↗
PubMed1981

Oxanosine, a novel nucleoside from actinomycetes.

Oxanosine, a novel nucleoside, has been isolated from the culture filtrate of a strain of Streptomyces. The structure was determined to be 5-amino-3-beta-D-ribofuranosyl-3H-imidazo [4,5-d] [1,3]oxazin-7-one by X-ray crystallographic analysis and chemical studies. Oxanosine showed weak antibacterial activity on peptone agar; for example, Escherichia coli K-12 (MIC 12.5 mcg/ml). The antibacterial activity was antagonized by addition of guanine, guanosine and 5'-guanylic acid. Oxanosine inhibited the growth of HeLa cells in vitro (IC50 32 mcg/ml) and suppressed the growth of L-1210 leukemia in mice. The primary action of oxanosine appears to be inhibition of GMP-synthetase. Intravenous injection of 4 mg of oxanosine to mice does not show any toxic sign.

Cell Division↗