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Biomedical subjects

N Shibata

Publications and source records attributed to N Shibata.

At least 361 records · Page 20Linked to original sources
PubMed1977

Electron microscopic studies on the myofibrils in the epithelial cells of the Bowman's capsule and of proximal tubules in rat renal cortex (first report).

Two kinds of filaments in parallel arrangement have been observed in the basal part of the cytoplasm in the epithelial cells of a Bowman's capsule and in the epithelial cells of a proximal tubule of a glycerinated rat kidney. The first was thin and linear in shape, 55-70 A in diameter and exhibited arrow-head structures upon treatment with heavy meromyosin (HMM). The other was rod shaped, with thick filaments, 110-130 A in diameter, 0.14-0.16 micrometer in length, few in number scattered among the thin filaments. From the non-glycerinated kidney, bundles of fibers were attached to some electron-dense regions inside the cell membrane. In highly magnified pictures, two kinds of filaments could be observed. One kind was thin, attached to dense regions, and the other thick, and not attached to dense regions. It is conceivable that these filaments correspond to myofibrils from their characteristic electron microscopical features. An electron microscopical analogy detected in both smooth muscle cells and renal cells leads us to speculate that these thin and thick filaments consisted of actin and myosin respectively.

Animals↗
PubMed1976

Bioenergetics in clinical medicine. VIII. Adminstration of coenzyme Q10 to patients with essential hypertension.

Coenzyme Q10 has been administered to five patients having essential hypertension and deficiencies of activity of succinate dehydrogenase-co-enzyme Q10 reductase in leucocyte preparations ranging from 20-40%. For a 74-year old male, the systolic pressure was reduced (p less than 0.001), the diastolic pressure was reduced (p less than 0.05), the specific activity of the coenzyme Q10-enzyme was increased (p less than 0.001), and the deficiency of coenzyme Q10 activity was negated (p less than 0.01). Four patients receiving CoQ10 for 3-5 months showed reductions (p less than 0.05 to p less than 0.001) of diastolic pressure, and 3 of these 4 showed reductions (p less than 0.05 to p less than 0.01) of diastolic pressure. Initial deficiencies of enzyme activity were reduced (p less than 0.01 to 0.05) in two patients. Three other patients did not show the high level of deficiency on treatment as initially observed. These effects of CoQ10 on the reduction of systolic and diastolic blood pressures, increase in CoQ10-enzyme activity, and reduction of CoQ10-deficiency are presumably due to improved bioenergetics through correction of a deficiency of coenzyme Q10.

Adult↗
PubMed1975

Leucocyte myosin and its location in the cell.

The intracellular location of the binding site of antibody against purified myosin prepared from equine leucocytes was investigated in neutrophils and lymphocytes by electron microscopy using peroxidase-labelled antibody method. The myosin extracted from equine leucocytes could bind skeletal muscle F-actin and the formed complex showed the biophysical and biochemical properties and electron microscopic appearance of actomyosin. On immunodiffusion, the leucocyte myosin formed a single precipitin line with its antibody prepared in rabbits. The antibody also formed single precipitin lines with myosins from lymphocytes and thrombocytes, fusing with each other. The antibody against the leucocyte myosin did not react with myosins from skeletal or arterial smooth muscle. The specificity of the antibody was further established by determination of K+-EDTA-activated ATPase activity remained in the supernate of antigen-antibody mixture. Under electron microscope, the intracellular immunoreactive products of peroxidase labelled antibody were found in cytoplasm of neutrophils and lymphocytes incubated with antibody against leucocyte myosin, but not in neutrophils or lymphocytes treated with IgG from normal rabbits.

Adenosine Triphosphatases↗
PubMed1975

Scanning electron microscopic studies on the formation of cardiac intercellular connection in culture.

The fine surface structural appearance of the cardiac intercellular connection was studied scanning electron microscopically in culture. When individual heart cells contract each other, they start to beat synchronically. On the base of scanning electron microscopic observation, the early pattern of the formation of it could be suggested as follows: five ramified processes extend from the edge of the cells, and the ends of these processes bridge each other to form the intercellular connection.

Animals↗
PubMed1975

Bioenergetics in clinical medicine. Studies on coenzyme Q10 and essential hypertension.

The specific activities (S.A.) of the succinate dehydrogenase-coenzyme Q10 (CoQ10) reductase of a control group of 65 Japanese adults and 59 patients having essential hypertension were determined. The mean S.A. of the hypertensive group was significantly lower (p less than 0.001) and the mean % deficiency of enzyme activity was significantly higher (p less than 0.001) than the values for the control group. These data on Japanese in Osaka agree with data on Americans in Dallas. Some patients showed no CoQ10-deficiency, and others showed definite deficiencies. Emphasizing the CoQ10-enzyme for patient selection, CoQ10 was administered to hypertensive patients. Four individuals showed significant but partial reductions of blood pressure. Monitoring the CoQ10-enzyme before, during, and after administration of CoQ10 indicated responses. The maintenance of high blood pressure could be primarily due to contraction of the arterial wall. Contraction or relaxation of an arterial wall is dependent upon bioenergetics, which also provide the energy for biosynthesis of angiotensin II, renin, aldosterone, and the energy for sodium and potassium transport. A clinical benefit from administration of CoQ10 to patients with essential hypertension could be based upon correcting a deficiency in bioenergetics, and point to possible combination treatments with a form of CoQ and anti-hypertensive drugs.

Adult↗
PubMed1975

Morphological and biochemical studies on the heart of the cardiomyopathic Syrian hamster.

Histological and ultrastructural observations of embryonic hearts and cultured cells from hamsters with inherited cardiomyopathy (BIO 14.6 line) showed a significant delay in maturation of the sarcomeric units in comparison with those of unrelated healthy control hamsters. Phase-contrast microscopic observation of the cultured cardiomyocytes revealed more rapid diminution of beating frequencies in the diseased hamsters than in the controls. Negatively stained ultrastructure and yield of the actomyosin extracted both from the cardiomyopathic and the control hamsters showed no fundamental differences. Disc electrophoresis of the erythrocyte ghosts revealed, at least, a quantitative difference in one of the composing proteins between these two groups.

Actomyosin↗