Biomedical subjects

N Salama

Publications and source records attributed to N Salama.

21 records · Page 2Linked to original sources

PubMedDate not supplied

Microvascular testicular blood flow as evaluated by laser Doppler flowmetry after the surgical induction of varicocele.

This study was conducted to evaluate the changes in microvascular testicular blood flow (microvascular TBF), which may be associated with varicocele. The pathology was developed through partial ligation of left renal vein in 4 groups of rats. Controls of each group underwent sham surgery. It was observed that microvascular TBF dropped and its vasomotion became inhibited once the left renal vein was partially ligated as studied by laser Doppler flowmetry (LDF). Four to five minutes later, blood flow rose to the pretreatment level and that of controls. Vasomotion reappeared with a uniform frequency and amplitude. At different periods of varicocele bearing (1, 3, 6, and 14 weeks) microvascular TBF was reevaluated. Vasomotion appeared intact without any abnormalities in the 4 groups. To examine the response of testicular microvasculature after surgical creation of the varicocele to pharmacological stimulation another group of animals received human chorionic gonadotropin (hCG) treatment 6 weeks after varicocele creation. Vasomotion was inhibited in these animals. In conclusion, LDF is a suitable tool to evaluate microvascular TBF in vivo. It can detect acute changes in blood flow that may happen at surgical induction of varicocele. In addition, the vasculature of the testis with a surgically induced varicocele can still respond to hCG stimulation.

Animals↗

PubMedDate not supplied

Transforming growth factor (beta1) in testes of aged and diabetic rats: correlation with testicular function.

This study was conducted to evaluate the expression of transforming growth factor-beta1 (TGF-beta1) in testis with aging and progress of diabetes mellitus (DM) and correlated this with testicular function. Zucker diabetic fatty rats (15, 40, and 75 weeks old) were used as models for non-insulin-dependent DM. The controls were age-matched nondiabetic Zucker fatty rats. Serum testosterone levels and daily sperm production were evaluated. Testicular tissue was immunostained with anti-TGF-beta1. Labeled cells were assessed and the staining intensity was estimated. The 15-week control and diabetic animals showed the lowest levels of staining intensity but this started to increase significantly in the older animals of both categories. However, the diabetic animals had significantly deeper intensities relative to controls at the different age groups. These changes in TGF-beta1 expression were negatively correlated with both testosterone and sperm production. Therefore, aging and DM enhanced the expression of TGF-beta1 in testis, and this was parallel with progress of both conditions, although DM seems to be predominant. These alterations in TGF-beta1 expression may be related to changes in testicular function associated with both conditions.

Aging↗

PubMedDate not supplied

Brain nitric oxides synthase in major pelvic ganglia of aged (LETO) and diabetic (OLETF) rats.

This study was conducted to evaluate the effects of aging and diabetes mellitus (DM) on brain nitric oxide synthase (bNOS) expression in major pelvic ganglia (MPG) of rats. Otsuka Long Evans Tokushima Fatty rats (12, 30, and 70 weeks old), which are genetic models with non-insulin-dependent DM (NIDDM), and age-matched nondiabetic Long Evans Tokushima Otsuka controls were used. The MPG of all rats in this study were subjected to cryo-sectioning and staining with bNOS polyclonal AB and rhodamine-conjugated rabbit IgG. Fluorescence intensities of the stained neurons were assessed in randomly selected fields per each specimen. Animals of both groups revealed significant decline in the staining intensity of their neurons with aging and the progress of DM, but diabetic rats showed more decline than controls. In conclusion, both aging and NIDDM could decrease bNOS expression in rat MPG. However, NIDDM has a more evident effect than aging on that expression. The decrease in bNOS may cause a disturbance in functions of the target pelvic structures of these ganglia under both conditions.

Aging↗