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Biomedical subjects

N S Mel'nik

Publications and source records attributed to N S Mel'nik.

6 recordsLinked to original sources
PubMed1984

[Use of paper indicator systems for the rapid identification of Corynebacterium diphtheriae, Neisseria meningitidis and the genus Staphylococcus].

The possibility of using paper indicator discs with glucose, saccharose, lactose and urea, manufactured at the Gorky Research Institute of Epidemiology and Microbiology, has been studied and the methods for the preparation of indicator discs with fructose, maltose, starch and sodium phenolphthalein-phosphate for the biochemical identification of C. diphtheriae, N. meningitidis and staphylococci have been proposed. The study of 395 C. diphtheriae strains, 98 N. meningitidis strains and 328 staphylococcal strains has shown that paper indicator systems are highly sensitive and specific, which makes it possible to recommend their introduction in laboratory practice for the rapid identification of the above-mentioned organisms.

Bacteriological Techniques↗
PubMed1982

[Accelerated identification method for Corynebacterium diphtheriae].

A method for rapid isolation of C. diphtheriae and determination of its biovar is proposed. The method consists in the inoculation of the material under study into 5% blood agar, the determination of cystinase in the modified Pisou medium prepared from agar D with sodium hyposulfite added and the fermentation of starch in dishes with serum starch agar. The proposed method allows one to reduce the time necessary for the identification of C. diphtheriae by 18 hours.

Bacteriological Techniques↗
PubMed1981

[Use of ristomycin diffused into the culture medium for separation of meningococci from nasopharyngeal mucus].

The method for enhancing the growth of meningococci after the inoculation of the specimens of nasopharyngeal mucus is proposed. The method is based on the diffusion of ristomycin into agar from ristomycin-impregnated filter paper strips. During the study of 694 specimens of nasopharyngeal mucus the above-mentioned method allowed isolation of 108 meningococcal cultures, while in serum agar only 21 cultures were isolated. The effectiveness coefficient of this method was found to be 80.6%, its effectiveness index being 5.1. The method facilitated the isolation of meningococci and in some cases reduced the time of analysis by 1 day.

Culture Media↗