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Biomedical subjects

N R Pace

Publications and source records attributed to N R Pace.

At least 145 records · Page 8Linked to original sources
PubMed1976

Nucleotide sequence of 5 S ribosomal RNA precursor from Bacillus subtilis.

The complete nucleotide sequence of a 179-nucleotide precursor (p5A) of 5 S ribosomal RNA from Bacillus subtilis is presented. In addition to the 116-nucleotide mature segment, the p5A molecule contains 21 additional nucleotides at its 5' end and 42 at its 3' terminus. Structural features within the p5A molecule which possibly interact with a specific maturation endonuclease (RNase M5) are identifiable. These include 2-fold rotational symmetry about the cleavage sites, which may approximately place the p5A molecule on the RNase M5 surface, and translational (positional) symmetry, which may precisely orient substrate phosphodiester bonds with respect to catalytic amino acids. Certain features of the 3' -terminal, precursor-specific portion of p5A possibly are involved in the termination of DNA transcription.

Bacillus subtilis↗
PubMed1974

The 7S RNA common to oncornaviruses and normal cells is associated with polyribosomes.

The 7S RNA species first demonstrated in avian and murine oncornaviruses and later in normal, uninfected cells is found associated in part with cellular polyribosomes. A molar ratio of 7S RNA to 5S ribosomal RNA of 0.05 indicates that there is approximately one mole of 7S RNA per mole of messenger RNA. Dissociation of polyribosomes with dimethylsulfoxide results in a marked decrease in the sedimentation rate of the 7S RNA. The dimethylsulfoxide-induced dissociation of polyribosomes and the concomitant movement of the 7S RNA from the polyribosome region into lighter regions of a sucrose gradient are both inhibited by cycloheximide, indicating that the 7S RNA is indeed associated with polyribosomes and not with a ribonucleoprotein particle sedimenting with polyribosomes.

Animals↗
PubMed1971

Gene dosage for 5S ribosomal ribonucleic acid in Escherichia coli ad Bacillus megaterium.

The number of gene copies for 5S ribosomal ribonucleic acid (rRNA), relative to that for 16 and 23S rRNA, has been determined by deoxyribonucleic acid (DNA)-RNA hybridization for Escherichia coli and Bacillus megaterium. In both cases, the number of 5S rRNA genes equals the number of 16 or 23S rRNA genes. Rapid procedures for preparing extremely highly purified DNA suitable for DNA-RNA hybridization experiments and chemically pure 5S rRNA are described.

Acrylates↗
PubMed1970

Formation of all stable RNA species in Escherichia coli by posttranscriptional modification.

The kinetics of accumulation of the known stable RNA species (23S, 16S, and 5S rRNA and tRNA) in Escherichia coli C122 were monitored by polyacrylamide gel electrophoresis of purified cellular RNA, following termination of brief pulse labeling with (32)P-orthophosphate. Isotopically labeled stable RNA species appear only after a time lag, while total cellular RNA and the ostensible precursors to the stable RNA classes accumulate from the earliest times examined. It is concluded that all the known stable RNA species in E. coli are the products of posttranscriptional modification.

Electrophoresis, Disc↗